Formulation, Characteristics and Viability Probiotics Cell of Biscuit Production from Hotong Flour (Setaria italica (L) Beauv.), Walnut (Canarium indicum L.), Enriched by Probiotic Cream
![](/public/site/images/admin/icons/icon-doi.png)
Jeirel Wattilete(1), Dwi Larasatie Nur Fibri(2*), Endang Sutriswati Rahayu(3)
(1) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
(2) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
(3) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia; University Center of Excellence for Integrated Research and Application for Probiotic Industry, Universitas Gadjah Mada, Jl. Teknika Utara, Caturtunggal, Yogyakarta 55281, Indonesia; Center for Food and Nutrition Studies, Universitas Gadjah Mada, Jl. Teknika Utara, Caturtunggal, Yogyakarta 55281, Indonesia
(*) Corresponding Author
Abstract
Hotong (Setaria italica (L) Beauv.) and walnut (Canarium indicum L.) are nutrient-rich local foods from Maluku Province, Indonesia, significant health. In this context, biscuit enriched with probiotic cream are a recommended snack for enhancing nutrition and promoting balance of the intestinal microflora, at a sufficient quantity of 107 log CFU/g. Therefore, this study aimed to objectively evaluate the physicochemical and organoleptic characteristics of biscuit, as well as assess probiotic viability. The used was a completely randomized design (CRD) with three different formulation treatments containing hotong flour and walnut at ratios of 40%:10% (F1), 35%:15% (F2), and 30%:20% (F3). Probiotic cell viability was analyzed at temperatures of 20 °C, 37 °C, and 45 °C. The results showed that F3 had the most favorable formulation regarding nutritional adequacy rate with 173 kcal energy, 10% fat, 4.41% protein, 5.20% carbohydrate, and 2 g sugar. It featured a crisp texture and increasingly darker colors, in line with the outcomes of sensory evaluations. F3 was the preferred, achieving high scores for color (5.38), taste (6.13), aroma (5.13), breakability (5.86), crispness (6), aftertaste (5.02), and overall quality (6.03). Cell viability for all treatments was higher at 20 °C and remained above 107 log CFU/g.
Keywords
Full Text:
PDFReferences
AOAC. (2005). Official Methods of Analysis Association of Official Analytical Chemists. Inc. Arlington Virginia.
Arenas, E.H. & Trinidad, T.P. (2016). Fate of polyphenols in pili (Canarium ovalum Engl.) pomace after in vitro simulated digestion. Asian Pacific Journal of Tropical Biomedicine, 7, 53-58. https://doi.org/10.1016/j.apjtb.2016.11.002.
Aril-dela Cruz, J.V., Bungihan, M.E., dela Cruz, T.E.E., & Sagum R.S. (2018). Canarium ovalum Engl. (Pili) exocarp crude extract as functional food colorant incorporated in yogurt developed product. Food Research, 2, 89-98. https://doi.org/10.26656/fr.2017.2(1).173.
BAM (Bacteriological Analytical Methods) Online. (2001). hĴp://www.cfsan.fda.gov/~ebam.html. [7 Agustus 2023].
Dave, R. I., and Shah, N. P. (1997). Viability of Yaghurt and Probiotics Bacteria in Yogurts Made from Commercial Starter Cultures. Int. Dairy Journal 7(1):31-41 https://doi.org/10.1016/S0958-6946(96)00046-5
Djarkasi, G.S, S. Raharjo, Z. Noor dan S. Sudarmadji. (2007). Sifat Fisik dan Kimia Minyak Kenari. Agritech 27(4):165-175 https://doi.org/10.22146/agritech.9857.
FAO/WHO (Food Agriculture Organization/World Health Organization), 2002. Report of a Joint FAO/WHO Expert Consultation on Drafting Guidelines for.
Giuberti, G. (2015). Gluten Free Maize Cookies Prepared With High-Amylose Starch : In Vitro Starch Digestibility and Sensory Characteristics. J Nutr Food Sci; 5(6): 6–10. http://dx.doi.org/10.4172/2155-9600.1000424.
Herodian, S. (2008). Pengembangan Buru Hotong (Setaria Italica (L)Beauv) Sebagai Sumber Pangan Pokok Alternatif. Jurnal Pangan., XVII(52);1–15.
Irferamuna, A., Yulastri, A. and Yuliana (2019). Formulasi Biskuit Berbasis Tepung Jagung sebagai alternatif cemilan bergizi. Jurnal Ilmu sosial dan Humaniora 8(2);221-226 https://doi.org/10.23887/jish-undiksha.v8i2.21999.
Katresna, NP. (2017). Pengaruh Substitusi Tepung Modifikasi Sorgum (Shorgum bicolor L.) dan Terigu dengan Penambahan Bekatul Beras (Oryzae sativa L.) terhadap Karakteristik Cookies.Skripsi Universitas Pasundan. Bandung.
Lawalata, V.N. (2004). Kajian pemanfaatan kenari (Canarium ovatum) untuk meningkatkan nilai gizi sagu mutiara. [Tesis]. Bogor : Sekolah Pascasarjana, Institut Pertanian Bogor.
Lawalata, V.N., I. Maatoke, dan G. Tetelepta. (2019). Karakteristik kimia food bar puree pisang tongka langit (Musa troglodytarum) dengan penambahan kenari (Canarium indicum L.). Agritekno 8: 48-52. DOI: 10.30598/jagritekno.2019.8.2.48.
Leaky, R., Fuller, S., Teloar, T., Stevenson, L., Hunter, D., Nevenimo, T., Binifa, J., & Moxon, J. (2008). Characterization of tree-to-tree variation on morphological, nutritional, and medicinal properties canarium indicum nut. Agroforestry System An International Incorporating Agroforesty Forum.73(1) http://dx.doi.org/10.1007/s10457-007-9103-4.
Laswati, D, T. (2017). Masalah gizi dan peran gizi seimbang. Agrotech. Vol 2(1). https://doi.org/10.37631/agrotech.v2i1.12.
Mailoa, M. (2015). Kajian Senyawa Bioaktif Buah Kenari Segar (Canarium Vulgare Leenh). Prosiding Seminar Agroindustri dan Lokakarya Nasional FKPT-TPI Program Studi TIP-UTM.
Makanoneng, V,S., Nurali, E, J, N., Djarkasi, G, S, S. (2017). Pengembangan biskuit kenari (Canarium indicum L) berbahan baku tepung sagu baruk (Arenga microcarpa). Cocos. 8(3) 1-7. https://doi.org/10.35791/cocos.v1i2.14916.
Oktavia, F., Dwi Argo, B., Lutfi. (2014). Hidrolisis Enzimatik Ampas Tebu (Bagasse) Memanfaatkan Enzim Selulase dari Mikrofungi Trichoderma reseei dan Aspergillus niger Sebagai Katalisator dengan Pretreatment Microwave. Jurnal Keteknikan Pertanian Tropis dan Biosistem. 2(3);256-262.
Ostermann-porcel, M. V, A. N. Rinaldoni, L. T. Rodriguez-furlán, and M. E. Campderrós. 2016. Quality assessment of dried okara as a source of production of gluten-free flour. J Sci Food Agric. 97(7);2934-2941 https://doi.org/10.1002/jsfa.8131.
Prasetyo, A., Dwi, I., & Dian, R, A. (2014). Pemanfaatan tepung jagung (zea mays) sebagai pengganti tepung terigu dalam pembuatan biskuit tinggi energi protein dengan penambahan tepung kacang merah (Phaseolus vulgaris L). Jurnal teknosains pangan, 3(1). 15-22.
Pratiwi, H. (2015). Pengaruh Pemberian Biskuit Lele (Clarias gariepinus) Dengan Krim Probiotik Enterococcus faecium IS-27526 Terhadap Profil Lipid Dan Berat Badan Wanita Lansia. [Tesis]. Bogor (ID) :Sekolah Pascasarjana, Institut Pertanian Bogor.
Rahayu, E.S. et al., (2021). effect of probiotic Lactobacillus Plantaraum DAD-13 Powder Consumption on the gut microbiota and intenstinal healt of overweight adults. World Journal of Gastroenterology 125(1), hlm. 107-128. doi: 10.3748/WJG.V27.I1.10.
Rauf, R., Sarbini, D., & Nurdiana. 2017. Optimization of Fermentation Time on Physical Characteristics and Sensory Acceptance of Bread from Composite Wheat and Cassava Flours with Proporsional Water Volume. In International Conference on Science, Technology, and Humanity. Surakarta, Indonesia.
Santivarangkna, C., Kulozik, U., & Foerst, P. (2008). Inactivation mechanisms of lactic acid starter cultures preserved by drying processes. In Journal of Applied Microbiology (Vol. 105, Issue 1, pp. 1–13). Blackwell Publishing Ltd. https://doi.org/10.1111/j.1365-2672.2008.03744.x.
Sen, S.M., Karadeniz, T., (2015). The nutritional value of walnut. J. Hyg. Eng. Des. 11, 68–71.
Sigmarlatu. T., 2019. Pengaruh Modifikasi Menggunakan Sodium TripholyPhosphate terhadap karakteristik Tepung Buru Hotong (Setaria italic (L) beauv.) Skripsi. Jurusan Teknologi Hasil Pertanian Fakultas Pertanian Universitas Pattimura Ambon.
Statista Global Consumer. (2020). Media Usage By Channel In Indonesia 2020. https://www.statista.com/fore casts/823441/media-usage-inindonesia.
Sugiyono, S.E. 2010. Pengembangan Produk mi Instandari tepung Hotong (Setaria italica Beauv.) dan Pendugaan Umur Simpannya dengan Metode Akselerasi. Jurnal Teknologi dan Industri Pangan: 21(1);45-50.
Sulistyaningrum, A., Rahmawati dan Aqil, M. (2017). Karakteristik Tepung Jewawut (Foxtail Millet) Varietas Lokal Majene dengan Perlakuan Perendaman. Jurnal Penelitian Pascapanen Pertanian, 14(1);11–21.
Thomson, L.A.J. dan B. Evans. (2004). Canarium indicum var. indicum and C. barveyi (Canarium nut). Species Profiles for Pacific Island Agroforestry. Version 1.1. diakses tanggal 06 September 2022 dari http://www.traditionaltree.org.
Tuhumury, H.C.D., Souripet, A., & Maadara, A. (2020). Canarium nut powder formulations for making cookies crispy. IOP Conf. Series: Journal of Physics: Conf. Series 1463, 012028. http://doi.org/10.1088/1742- 6596/1463/1/012028.
Vivek, K., Mishra, S., Pradhan, R. C., Nagarajan, M., Kumar, P. K., Singh, S. S., Manvi, D., & Gowda, N. N. (2023). A comprehensive review on microencapsulation of probiotics: technology, carriers and current trends. Applied Food Research, 3(1), 100248. https://doi.org/10.1016/j.afres.2022.100248.
Winarno, F. (1997). Kimia Pangan dan Gizi. jakarta: PT. Gramedia Pustaka Utama.
Yamaguchi, S and Minomiya, K. (1998). What is umami. Food Rev. Int. 14 (2&3):123-138.
![](/public/site/images/admin/icons/icon-doi.png)
Article Metrics
![](/public/site/images/admin/icons/icon-graph.png)
![](/public/site/images/admin/icons/icon-pdf.png)
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Dwi Larasatie Nur Fibri, Jeirel Wattilete, Endang Sutriswati Rahayu
![Creative Commons License](http://i.creativecommons.org/l/by-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
agriTECH has been Indexed by:
agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.