* Corresponding Author
Karakteristik Minuman Sari Lemon (Citrus limon) dengan Penambahan Konsentrasi Kolagen yang Berbeda
https://doi.org/10.22146/agritech.59724 Lola Anandya Inke(1*), Ahmad Sapta Zuidar(2), Dyah Koesoemawardani(3), Siti Nurdjanah(4)
(1) Jurusan Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof. Dr. Soemantri Brojonegoro No. 1, Gedong Meneng, Bandar Lampung, Lampung 35145
(2) Jurusan Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof. Dr. Soemantri Brojonegoro No. 1, Gedong Meneng, Bandar Lampung, Lampung 35145
(3) Jurusan Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof. Dr. Soemantri Brojonegoro No. 1, Gedong Meneng, Bandar Lampung, Lampung 35145
(4) Jurusan Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof. Dr. Soemantri Brojonegoro No. 1, Gedong Meneng, Bandar Lampung, Lampung 35145
(*) Corresponding Author
Abstract
Minuman sari lemon kolagen merupakan salah satu minuman fungsional yang mudah diserap oleh tubuh, namun kolagen yang berasal dari ikan memiliki aroma amis sehingga dapat dikombinasikan dengan sari buah lemon untuk menutupi aroma amis tersebut. Penelitian ini bertujuan untuk memperoleh konsentrasi kolagen yang tepat sehingga menghasilkan minuman sari lemon kolagen berbasis sari buah lemon dengan karakteristik fisikokimia, mikrobiologi, dan sensori terbaik. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan 6 perlakuan level konsentrasi kolagen yang berbeda, yaitu 0,5% (K1), 1% (K2), 1,5% (K3), 2% (K4), 2,5% (K5), dan 3% (K6) dengan 4 kali ulangan. Proses pembuatan dilakukan dengan mencampurkan sari lemon dan gula pasir, kemudian ditambahkan kolagen dan air yang sudah disaring dengan UV dan dipanaskan sambil diaduk hingga suhu ± 60 °C, lalu dimasukkan ke dalam botol kaca dan dilakukan pasteurisasi dengan suhu ± 75-85 °C selama 10 menit. Hasil penelitian menunjukkan bahwa konsentrasi kolagen berpengaruh nyata terhadap karakteristik fisik (tingkat kekeruhan, viskositas, dan pH), karakteristik mikrobiologi (total mikroba), dan karakteristik sensori. Minuman sari lemon kolagen berbasis sari buah lemon terbaik diperoleh pada konsentrasi kolagen 1,5% (K3) dengan aroma agak khas lemon (3,11), rasa yang khas lemon (3,71), warna putih sedikit kekuningan (2,34), penerimaan keseluruhan suka (3,53), persen transmittan 67,20%, viskositas 1,19 cP, pH 4,07, dan total mikroba 1,2 x 10 2 koloni/mL. Minuman sari lemon kolagen ini mengandung kadar protein sebesar 1,34 ± 0,04%, kadar vitamin C sebesar 0,018 ± 0,00 mg/mL, dan aktivitas antioksidan sebesar 19,39 ± 0,08%.
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Anto, J., Iskandar, & Rizal, A. (2018). Physico-Chemical Characteristics and Levels of Preference for Drinking Collagen Drinks the Result of Extracts from Nilem Fish Skins. Journal of Aquaculture Research & Development, 9(10), 1–4. https://doi.org/10.4172/2155-9546.1000555
AOAC International. (1990). 981.12 pH for Acidified Foods. In Official Methods of Analysis (p. 1230 pp). AOAC International.
ASTM International. (2009). Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (and Calculation of Dynamic Viscosity) (Patent No. D-445-06).
Bilek, S. E., & Bayram, S. K. (2015). Fruit juice drink production containing hydrolyzed collagen. Journal of Functional Foods, 14, 562–569. https://doi.org/10.1016/j.jff.2015.02.024
BSN. (1992). SNI 01-2891-1992 tentang Cara Uji Makanan dan Minuman. Badan Standarisasi Nasional.
BSN. (2014). Minuman Sari Buah - SNI 3719:2014. Badan Standarisasi Nasional.
Chatterrjee, G., De Neve, J., Dutta, A., & Das, S. (2015). Formulation and Statistical Evaluation of A Ready-To-Drink Whey Based Orange Beverage and Its Storage Stability. Revista Mexicana de Ingeniera Quimica, 14(2), 253–264.
Chen, Q. (2012). Co-Encapsulation of Fish Oil With Phytosterol Esters and Limonene. Thesis. University of Auckland.
Darmanto, Y. S., Agustini, T. W., & Swastawati, F. (2012). Efek Kolagen dari Berbagai Jenis Tulang Ikan terhadap Kualitas Miofibril Protein Ikan Selama Proses Dehidrasi. Teknologi Dan Industri Pangan, 23(1), 36–40.
Destiana, A. L. & Sari, S. H. (2018). Ekstraksi kolagen ikan manyung dan ikan kakap sebagai alternatif kolagen. Bio-site, 4(2), 48-52.
Dev, C., & Nidhi, S. R. R. S. (2016). Basketful Benefit of Citrus Limon. International Research Journal of Pharmacy, 7(6), 1–4. https://doi.org/10.7897/2230-8407.07653
Edet, E., Owai, O., Atangwho, I., Ofem, O., & Anaka, D. (2017). Comparative In Vitro Antioxidant Properties of Water Juice from Selected African Fruits Consumed in Calabar, Cross River State (Crs), Nigeria. Global Journal of Pure and Applied Science, 23, 81–86. https://doi.org/https://dx.doi.org/10.4314/gjpas.v23i1.9
Ekawati, E. & Darmanto, W. (2019). Lemon (Citrus limon) Juice has Antibacterial Potential Against Diarrhea-Causing Pathogens. IOP Conference Series: Earth and Environmental Science. https://doi.org/doi:10.1088/1755-1315/217/1/012023
El-Ishaq, A., & Obirinakem, S. (2015). Effect of Temperature and Storage on Vitamin C Content in Fruits Juice. International Journal of Chemical and Biomolecular Science, 1(2), 17–21.
Ennaas, N., Hammami, R., Gomaa, A., Bedard, F., Biron, E., Subirade, M., Beaullieu, L., & Fliss, I. (2016). Collagencin, an Antibacterial Peptide from Fish Collagen: Activity, Structure, and Interaction Dynamics with Membrane. Biochemical and Biophysical Research Communications, 473(2), 642–647. https://doi.org/https://doi.org/10.1016/j.bbrc.2016.03.121
Fitriyana, R. A. (2017). Perbandingan Kadar Vitamin C pada Jeruk Nipis (Citrus aurantiifolia) dan Jeruk Lemon (Citrus limon) yang Dijual di Pasar Linggapura Kabupaten Brebes. Publikasi Ilmiah Civitas Akademika Pioliteknik Mitra Karya Mandiri Brebes, 2(2), 1–10.
Geri, J. D., Ayu, D. F., & Harun, N. (2019). Kombinasi Minuman Lidah Buaya Berkarbonasi dengan Sari Lemon. Jurnal Agroindustri Halal, 5(2), 132–140.
Gomez-Guillen, M. C., Gimenez, B., Lopez-Caballero, M. E., & Montero, M. P. (2011). Functional and Bioactive Properties of Collagen and Gelatin from Alternative Sources: A Review. Food Hydrocolloids, 25(8), 1813–1827. https://doi.org/10.1016/j.foodhyd.2011.02.007
Hashim, P., Mohd Ridzwan, M. S., Bakar, J., & Mat Hashim, D. (2015). Collagen in Food and Beverage Industries. International Food Research Journal, 22(1), 1–8.
Holm, K. (2006). The Relations Between Food Structure and Sweetness. Chalmers University of Technology.
Huda, N., Seow, E. K., Normawati, M. N., Nik Aisyah, N. M., Fazilah, A., & Easa, A. M. (2013). Effect of Duck Feet Collagen Addition on Physicochemical Properties of Surimi. International Food Research Journal, 20(2), 537–544.
JBT Corporation. (2018). Laboratory Manual: Procedures for Analysis of Citrus Products (Seventh). John Bean Technologies Corporarion Inc.
Karabagias, I. K. (2017). Volatile Compounds of Freshly Prepared Lemon Juice from the Region of Kalamata. SM Analytical and Bioanalytical Techniques, 2(2), 1–4. https://doi.org/10.36876/smabt.1013
Kittiphattanabawon, P., Sriket, C., Kishimura, H., & Benjakul, S. (2019). Characteristics of acid and pepsin solubilized collagens from nile tialpia (Oreochromis niloticus) scale. Emirates Journal of Food and Agriculture, 31(2), 95-101. https://doi.org/10.9755/ejfa.2019.v31.i2.1911
Krasnova, I. S., Semenov, G. V., & Zarubin, N. Y. (2020). Modern Technologies for Using Fish Wastes in the Production of Collagen Hydrolysates and Functional Beverages. IOP Conference Series: Earth and Environmental Science, 421(6). https://doi.org/10.1088/1755-1315/421/6/062030
Kusumaningtyas, E., Nurilmala, M., & Sibarani, D. (2019). Antioxidant and Antifungal Activities of Collagen Hydrolysates from Skin of Milkfish (Chanos chanos) Hydrolyzed using Various Bacillus Proteases. IOP Conference Series: Earth and Environmental Science, 278(1), 1–8. https://doi.org/10.1088/1755-1315/278/1/012040
Leon-Lopez, A., Perez-Marroquin, X. A., Campos-Lozada, G., Campos-Montiel, R. G., & Aguirre-Alvarez, G. (2020). Characterization of whey-based fermented beverages supplemented with hydrolyzed collagen: Antioxidant activity and bioavailability. Foods (Basel, Switzerland), 9(8), 1106. https://doi.org/10.3390/foods9081106
Maharani, S. A. (2018). Karakteristik Minuman Jeli dengan Fortifikasi Kolagen Kulit Ikan Tuna (Thunnus albacares). Skripsi. Institut Pertanian Bogor.
Nizhar, U. (2012). Level Optimum Sari Buah Lemon (Citrus limon) sebagai Bahan Penggumpal pada Pembentukan Curd Keju Cottage. Skripsi. Universitas Hasanudin.
Normah, I., & Maidzatul, A. M. (2018). Effect of Extraction Time on the Physico-chemical Characteristics of Collagen from Sin Croaker (Johniecop sina) Waste. International Food Research Journal, 25(3), 1074–1080.
Nuraini, F. & Nawansih, O. (2006). Uji Sensori. Universitas Lampung. Bandar Lampung. 121 hlm.
Omar, S. R., Rodzi, S. N. A. M., Talib, N. A. C., & Noor, N. H. M. (2016). Factor Affecting Consumer’s Intention towards Purchasing Halal Collagen Beauty Drink in Malaysia: a Structural Equation Modelling. Journal of Entrepreneurship and Business, 4(2), 1-16. https://doi.org/10.17687/JEB.0702.06.
Palupi, M. R., & Widyaningsih, T. D. (2015). Pembuatan Minuman Fungsional Liang Teh Daun Salam (Eugenia polyantha) dengan Penambahan Filtrat Jahe dan Filtrat Kayu Secang. Jurnal Pangan Dan Agroindustri, 3(4), 1458–1464.
Pavlovska, G., & Tanevska, S. (2013). Influence of Temperature and Humidity on the Degradation Process of Ascorbic Acid in Vitamin C Chewable Tablets. Journal of Thermal Analysis and Calorimetry, 111(3), 1971–1977. https://doi.org/10.1007/s10973-011-2151-z
Plavan, V., Koliada, M., Chen, W., & Barsukov, V. (2014). Extraction of Collagen From Fish Waste and Determination of Its Amino Acid Composition. ICAMS 2014 – 5th International Conference on Advanced Materials and Systems.
Puspitarini, T., Pratjojo, W., & Kusumastuti, E. (2014). Efektivitas Penggunaan Kulit Jeruk Nipis sebagai Penghilang Bau Amis pada Ikan. Indonesian Journal of Chemical Science, 3(2), 113–118.
Rifkowaty, E. ;, & Martanto. (2016). Minuman Fungsional Serbuk Instan Jahe (Zingiber officinale Rosc) dengan Variasi Penambahan Ekstrak Bawang Mekah (Eleutherine americana Merr) sebagai Pewarna Alami. Jurnal Teknik Pertanian Lampung, 4(4), 315–324.
Rum, S. S., Kawiji, & Setyaningrum, A. (2016). Kapasitas Antioksidan Minuman temulawak (Curcuma xanthorrhiza) Menggunakan Gula Kristal Putih, Gula Kristal Merah, Gula Merah, dan Gula Aren. Biofarmasi, 14(2), 39–46. https://doi.org/10.13057/biofar/f140201
Sady, M., Jaworska, G., Grega, T., Bernaś, E., & Domagaĺa, J. (2013). Application of Acid Whey in Orange Drink Production. Food Technology and Biotechnology, 51(2), 266–277.
Sharif, M. K., Butt, M. S., Sharif, H. R., & Nasir, M. (2017). Handbook of Food Science and Technology: Chapter 14 Sensory Evaluation and Consumer Acceptability. University of Agriculture. Faisalabad. 455 pp.
Sharma, H. P., Sharma, S., Vaishali, & Patel, H. (2014). Effect of Storage Conditions on the Bio-chemical Quality of Lemon Drink. Journal of Food Research and Technology, 2(4), 158–164. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.388.674&rep=rep1&type=pdf
Shon, J., Eun, J. B., Eo, J. H., & Hwang, S. J. (2011). Effect of Processing Conditions on Functional Properties of Collagen Powder from Skate (Raja kenojei) Skins. Food Science and Biotechnology, 20(1), 99–106. https://doi.org/10.1007/s10068-011-0014-9
Sudarmadji, S. (1981). Prosedur Analisa untuk Bahan Makan dan Pertanian. Liberti.
Suhono, L., Listyaningrum, N. P., Trowulan, E., & Andayani, T. R. (2019). Bahan Baku Olahan Hasil Perikanan. Pusat Pendidikan Kelatuan dan Perikanan. Jakarta.
Tohmadlae, P. & Hinsui, J. (2017). The removal of fishy odor from tilapia skin using for gelatin extraction. The JSFS 85th Anniversary-Commemorative International Symposium, 10015, 1-2.
Uçan, F., Ağçam, E., & Akyildiz, A. (2016). Bioactive Compounds and Quality Parameters of Natural Cloudy Lemon Juices. Journal of Food Science and Technology, 53(3), 1465–1474. https://doi.org/10.1007/s13197-015-2155-y
Wang, Q. J., Mielby, L. A., Junge, J. Y., Bertelsen, A. S., Kidmose, U., Spence, C., & Byrne, D. V. (2019). The Role of Intrinsic and Extrinsic Sensory Factors in Sweetness Perception of Food and Beverages: A Review. Foods, 8(6)(211), 1–26. https://doi.org/http://dx.doi.org/10.3390/foods8060211
DOI: https://doi.org/10.22146/agritech.59724
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