Isolasi Bakteri Penghasil Enzim Lipase Penghasil Alkali dari Kulit Hewan

https://doi.org/10.22146/agritech.19344

V.S. Pertiwi Rumiyati(1*), Retno Indrati(2)

(1) Balai Besar Litbang Kulit, Karet, dan Plastik (BBKKP) Yogyakarta
(2) Fakultas Teknologi Pertanian, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Twenty seven bacterial strains isolated an a medium containing 0.50% palm oil as substrate and 0.015% spirit blue as an indicator from several raw hides of chicken, goat and cow showed an extracellular lipase activity. Of these strains, 10 were higher lipase producers. Lipases produced from five of these 10 isolates were stable on alkaline conditions, ranging from pH 8.0 to 11.0. Furthermore, activity of these enzymes was not inhibited by the presence of non-ionic, cationic nor ionic surfactants at concentration of 0.05% or 0.10%. Conversely, some of these surfactans could even activate the enzyme.

Keywords


bakteri kulit ayam; kulit sapi dan Lint kambing; lipase alkali; aktivitas; surfaktant

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DOI: https://doi.org/10.22146/agritech.19344

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agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.


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