Fermentasi Onggok Menggunakan Mutan Trichoderma untuk Produksi Selulase

https://doi.org/10.22146/agritech.9763

Ali Mursyid Wahyu Mulyono(1*), Muhammad Nur Cahyanto(2), Zuprizal Zuprizal(3), Zaenal Bachruddin(4)

(1) Fakultas Pertanian, Universitas Veteran Bangun Nusantara, Sukoharjo
(2) Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Jl. Sosio Yustisia, Yogyakarta 55281
(3) Fakultas Peternakan, Universitas Gadjah Mada, Jl. Agro, Yogyakarta 55281
(4) Fakultas Peternakan, Universitas Gadjah Mada, Jl. Agro, Yogyakarta 55281
(*) Corresponding Author

Abstract


The objective of the research was to study the influence of initial a of medium, inoculum concentration, initial pH of the medium and incubation time during fermentation of cassava bagasse by mutant Trichoderma AA1 on cellulase production. Fermentation of cassava bagasse was carried out by solid substrate fermentation method. The medium was inoculated by Trichoderma AA1 and incubated for four days. The initial a of medium (0.96, 0.97, 0.98, and 0.99), inoculum concentration (105, 106, 107, dan 108 spores/g), and initial pH of the medium (4.5, 5.0, 5.5, and 6.0) were stud- ied by measuring the cellulase activity during fermentation. The production of cellulase was the best when the medium had initial a of 0.99, inoculum concentration of 107 spores/g, and initial pH of 5. The peak of the cellulase production was achieved after 3-days fermentation. The cellulase activities obtained were 0.168 and 0.072 µmol/minute/ml forcarboxy methyl cellulase dan filter paper-ase respectively.

ABSTRAK

Penelitian ini bertujuan mempelajari pengaruh a awal medium, konsentrasi inokulum, pH awal, dan waktu fermentasi onggok menggunakan mutan Trichoderma AA1 terhadap produksi selulase. Fermentasi onggok menggunakan metode fermentasi substrat padat. Medium diinokulasi dengan mutan Trichoderma AA1 dan diinkubasikan selama 4 hari. Variabel yang dipelajari meliputi a awal medium (0,96; 0,97; 0,98; dan 0,99), konsentrasi inokulum (105, 106, 107, dan 108  spora/g), dan pH awal medium (4,5, 5,0, 5,5, dan 6,0.). Hasil penelitian menunjukkan bahwa kondisi terbaik fermentasi onggok menggunakan mutan Trichoderma AA1 untuk menghasilkan selulase adalah: a awal medium 0,99, konsentrasi inokulum 107 spora/g, pH awal medium 5, dan waktu fermentasi 3 hari. Aktivitas selulase yang dihasilkan adalah 0,168 dan 0,072 µmol/menit/ml masing-masing untuk carboxy methyl cellulase dan filter paper-ase.


Keywords


Cassava bagasse; Trichoderma; mutant; fermentation; cellulase

Full Text:

PDF



DOI: https://doi.org/10.22146/agritech.9763

Article Metrics

Abstract views : 440 | views : 1614

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Jurnal Agritech

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Agritech has been Indexed by:


Agritech (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.


website statisticsView My Stats