Effect of Sucrose and Starter on the Chemical and Organoleptic Properties of Fig Leaf Tea Water Kefir

https://doi.org/10.22146/agritech.82628

Muhammad Zakwan Saputra SY(1*), Agustin Krisna Wardani(2)

(1) Department of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas Brawijaya Malang, Jl. Veteran, Malang 65145, Jawa Timur
(2) Department of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas Brawijaya Malang, Jl. Veteran, Malang 65145, Jawa Timur
(*) Corresponding Author

Abstract


Water kefir is a fermented drink made by adding water kefir grains to a water solution containing dried fruit or sugar. This research aims to determine the effect of sucrose, starter, and interactions on the chemical characteristics (antioxidant activity, total acid, medium pH) and organoleptics of fig leaf tea water kefir and to determine the interaction of antioxidant activity with total acid and medium pH. The experiment used variations in starter concentration (5% and 8%) and sucrose concentration (3%, 6%, 9% and 12%). Water kefir was analyzed through chemical analysis (antioxidants, total acid, medium pH, and sugar content) and organoleptic tests (flavor, aroma, color, and overall preference). The results showed that variations in the addition of sucrose, starter, and interactions influenced the metabolic results (primary and secondary metabolites) of microorganisms in water kefir grains and statically had a significant effect on changes in chemical and organoleptic properties (flavor, aroma, and overall preference). The best chemical characteristic parameters were obtained in the B2S3 treatment (8% starter + 9% sucrose) with antioxidant activity of 51.27%, total acid of 0.70%, and medium pH of 3.53. Meanwhile, the best organoleptic parameters were obtained in the B2S4 treatment (8% starter + 12% sucrose) with a taste value of 5.56 (liked), an aroma value of 4.80 (slightly liked), a color value of 4.97 (slightly liked), and the overall liking level was 5.45 (liked), and the antioxidant activity of fig leaf tea water kefir had a strong relationship with total acid and medium pH. Furthermore, the addition of sucrose and different starters during the water kefir fermentation process affects the chemical and organoleptic properties of fig leaf tea water kefir. It is hoped that the results of this research will make fig leaf tea water kefir an alternative probiotic drink for sufferers of lactose intolerance and allergies to milk-based drinks.

Keywords


Antioxidant activity; fig leaf tea; organoleptic; water kefir

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References

Ahaddin, A. (2014). Isolasi dan Sitotoksisitas Ekstrak Flavonoid Daun Tin (Ficus carica Linn.). Institut Pertanian Bogor.

Alsayadi, M., Jawfi, Y. Al, Belarbi, M., & Sabri, F. Z. (2013). Antioxidant potency of water kefir muneer. Journal of Microbiology, Biotechnology and Food Sciences, 2(6), 2455–2461. https://office2.jmbfs.org/index.php/JMBFS/article/view/7101

Anggoro, O. E., Chrisnasari, R., & Dewi, A. D. R. (2022). Pembuatan Water Kefir dengan Memanfaatkan Madu dan Okra (Abelmoschus esculentus). KELUWIH: Jurnal Sains Dan Teknologi, 3(1), 10–23. https://doi.org/10.24123/saintek.v3i1.4903

Anjliany, M., Pangesti, R. F., & Mualim, I. A. (2022). Water kefir kulit buah naga: pengaruh dari starter dan lama fermentasi. Prosiding Seminar Nasional Lahan Suboptimal Ke-10 Tahun 2022, Palembang 27 Oktober 2022, 6051, 660–673.

Astuti, A., Rochmayani, M., & Aulia, R. (2018). NAWAKE (Nira Water Kefir): Pemanfaatan Nira Aren Sebagai Minuman Fungsional Kaya Probiotik. Agritech, 20(1), 1–6. https://doi.org/10.30595/agritech.v20i1.3416

Bertan, C. V., Dundu, A. K. T., & Mandagi, R. J. M. (2016). Pengaruh Pendayagunaan Sumber Daya Manusia (Tenaga kerja) terhadap Hasil Pekerjaan (Studi Kasus Perumahan Taman Mapanget Raya (Tamara). Jurnal Sipil Statik, 4(1), 13–20.

Cai, J. N., Choi, H. M., & Jeon, J. G. (2021). Relationship between sucrose concentration and bacteria proportion in a multispecies biofilm: Short title: Sucrose challenges to a multispecies biofilm. Journal of Oral Microbiology, 13(1). https://doi.org/10.1080/20002297.2021.1910443

Çevik, T., Aydoğdu, N. S., Özdemir, N., & Kök Taş, T. (2019). The Effect of Different Sugars on Water Kefir Grains. Turkish Journal of Agriculture - Food Science and Technology, 7, 40–45. https://doi.org/10.24925/turjaf.v7isp1.40-45.2687

Coban, H. B. (2020). Organic acids as antimicrobial food agents: applications and microbial productions. Bioprocess and Biosystems Engineering, 43(4), 569–591. https://doi.org/10.1007/s00449-019-02256-w

Cui, X. H., Chen, S. J., Wang, Y., & Han, J. R. (2013). Fermentation conditions of walnut milk beverage inoculated with kefir grains. LWT - Food Science and Technology, 50(1), 349–352. https://doi.org/10.1016/J.LWT.2012.07.043

Effendi, V. P., & Parhusip, A. J. N. (2022). Kajian Literatur Spesifikasi Mutu Fisikokimia Dan Mikrobiologis Water Kefir Dengan Variasi Konsentrasi Substrat Dan Starter. Jurnal Teknologi Pangan Dan Kesehatan (The Journal of Food Technology and Health), 3(2), 66–76. https://doi.org/10.36441/jtepakes.v3i2.558

Haryanti, P., & Mustaufik. (2020). Evaluasi Mutu Gula Kelapa Kristal (Gula Semut) di Kawasan Home Industri Gula Kelapa Kabupaten Banyumas. Jurnal Agroteknologi, 5(1), 48–61. https://jurnal.unej.ac.id/index.php/JAGT/article/view/19753

Hastuti, A. P., & Kusnadi, J. (2016). Organoleptic and Physical Characteristic of Red Rosella ( Hibiscus sabdariffa L . ) Kefir from Red Rosella Tea in The Market. Jurnal Pangan Dan Agroindustri, 4(1), 313–320. https://jpa.ub.ac.id/index.php/jpa/article/view/332

Ibrahim, M. M., & Yulianto, M. E. (2021). Optimasi Proses Ekstraksi Theaflavin dari Fermentasi Daun dengan Pancaran Sinar UV. Jurnal Pengabdian Vokasi, 2(2), 124–128. https://doi.org/10.14710/jpv.2021.12597

Irsalina, K., Dwiloka, B., & Setiani, B. E. (2018). Sifat Sensoris Cookies dengan Penambahan Daun Pepaya setelah Direbus. Jurnal Teknologi Pangan, 2(1), 24–26. https://ejournal3.undip.ac.id/index.php/tekpangan/article/view/19784/0

Isrianto, P. L. (2019). Biofermentasi Kefir Teh Pecut Kuda (Stachytarpheta jamaicensis) Sebagai Sumber Belajar Biologi. STIGMA: Jurnal Matematika Dan Ilmu Pengetahuan Alam Unipa, 12(02), 100–106. https://doi.org/10.36456/stigma.12.02.2057.100-106

Jaya, F. (2019). Ilmu, Teknologi dan Manfaat Kefir (1st ed.). Universitas Brawijaya Press.

Kandylis, P., Pissaridi, K., Bekatorou, A., Kanellaki, M., & Koutinas, A. A. (2016). Dairy and non-dairy probiotic beverages. Current Opinion in Food Science, 7, 58–63. https://doi.org/10.1016/j.cofs.2015.11.012

Khotib, I. M. (2018). Pengaruh Lama Fermentasi dan Variasi Konsentrasi Sari Buah Ciplukan (Physalis angulata Linn.) Terhadap Aktivitas Antioksidan, Total Bakteri Asam Laktat dan Mutu Kimia Kefir Air Sari Buah Ciplukan (Physalis angulata Linn.). UIN Maulana Malik Ibrahim Malang.

Kinteki, G. A., Rizqiati, H., & Hintono, A. (2019). Pengaruh Lama Fermentasi Kefir Susu Kambing Terhadap Mutu Hedonik, Total Bakteri Asam Laktat (BAL), Total Khamir dan pH. Jurnal Teknologi Pangan, 3(1), 42–50. https://doi.org/10.14710/jtp.2019.20685

Kumar, B. V., Vijayendra, S. V. N., & Reddy, O. V. S. (2015). Trends in dairy and non-dairy probiotic products - a review. Journal of Food Science and Technology, 52(10), 6112–6124. https://doi.org/10.1007/s13197-015-1795-2

Kusumastuti, B. R., Pantjajani, T., Kusumawardhany, P. A., Widjaja, L. K., Iswadi, H., & Dewi, A. D. R. (2022). Faktor Penting Preferensi Konsumen Pada Water Kefir Teh Ashitaba. MPI (Media Pharmaceutica Indonesiana), 4(1), 44–55. https://doi.org/10.24123/mpi.v4i1.4902

Lathif, Y. (2016). Pengaruh lama fermentasi dan variasi konsentrasi daun kersen (Muntingia calabura) Terhadap Total Asam, pH Medium Dan Aktivitas Antioksidan Kefir Air Teh Daun Kersen (Muntingia calabura L.). UIN Maulana Malik Ibrahim Malang.

Laureys, D., Aerts, M., Vandamme, P., & De Vuyst, L. (2018). Oxygen and diverse nutrients influence the water kefir fermentation process. Food Microbiology, 73, 351–361. https://doi.org/10.1016/j.fm.2018.02.007

Laureys, D., & De Vuyst, L. (2017). The water kefir grain inoculum determines the characteristics of the resulting water kefir fermentation process. Journal of Applied Microbiology, 122(3), 719–732. https://doi.org/10.1111/jam.13370

Laureys, D., Leroy, F., Hauffman, T., Raes, M., Aerts, M., Vandamme, P., & De Vuyst, L. (2021). The Type and Concentration of Inoculum and Substrate as Well as the Presence of Oxygen Impact the Water Kefir Fermentation Process. Frontiers in Microbiology, 12(February), 1–13. https://doi.org/10.3389/fmicb.2021.628599

Lestari, M. W., Bintoro, V. P., & Rizqiati, H. (2018). Pengaruh lama fermentasi terhadap tingkat keasaman, viskositas, kadar alkohol, dan mutu hedonik kefir air kelapa. Jurnal Teknologi Pangan, 2(1).

Li, C., Yu, M., Li, S., Yang, X., Qiao, B., Shi, S., Zhao, C., & Fu, Y. (2021). Valorization of Fig (Ficus carica L.) Waste Leaves: HPLC-QTOF-MS/MS-DPPH System for Online Screening and Identification of Antioxidant Compounds. Plants, 10(11), 2532. https://doi.org/10.3390/plants10112532

Lynch, K. M., Wilkinson, S., Daenen, L., & Arendt, E. K. (2021). An update on water kefir: Microbiology, composition and production. International Journal of Food Microbiology, 345(May), 109–128. https://doi.org/10.1016/j.ijfoodmicro.2021.109128

Mawa, S., Husain, K., & Jantan, I. (2013). Ficus carica L. (Moraceae): Phytochemistry, Traditional Uses and Biological Activities. Evidence-Based Complementary and Alternative Medicine, 2013, 1–8. https://doi.org/10.1155/2013/974256

Mulyani, S., Sunarko, K. M. F., & Setiani, B. E. (2021). Pengaruh Lama Fermentasi terhadap Total Asam, Total Bakteri Asam Laktat dan Warna Kefir Belimbing Manis (Averrhoa carambola). JURNAL ILMIAH SAINS, 21(2), 113–118. https://doi.org/10.35799/jis.21.2.2021.31416

Nielsen, B., Gürakan, G. C., & Ünlü, G. (2014). Kefir: A Multifaceted Fermented Dairy Product. Probiotics and Antimicrobial Proteins, 6, 123–135. https://doi.org/10.1007/s12602-014-9168-0

Patel, S. H., Tan, J. P., Börner, R. A., Zhang, S. J., Priour, S., Lima, A., Ngom-Bru, C., Cotter, P. D., & Duboux, S. (2022). A Temporal View Of The Water Kefir Microbiota And Flavour Attributes. Innovative Food Science & Emerging Technologies, 80(July), 103084. https://doi.org/10.1016/j.ifset.2022.103084

Piekarska-Radzik, L., & Klewicka, E. (2021). Mutual Influence Of Polyphenols And Lactobacillus spp. Bacteria In Food: A Review. European Food Research and Technology, 247(1), 9–24. https://doi.org/10.1007/s00217-020-03603-y

Prastujati, A. U., Hilmi, M., & Khirzin, M. H. (2018). Pengaruh Konsentrasi Starter Terhadap Kadar Alkohol, pH, dan Total Asam Tertitrasi (Tat) Whey Kefir. Jurnal Ilmu Peternakan Terapan, 1(2), 63–69. https://doi.org/10.25047/jipt.v1i2.893

Purba, A. P., Dwiloka, B., & Rizqiati, H. (2018). Pengaruh Lama Fermentasi terhadap Bakteri Asam Laktat (BAL), Viskositas, Aktivitas Antioksidan, dan Organoleptik Water Kefir Anggur Merah (Vitis vinifera L.). Jurnal Teknologi Pangan, 2(1), 49–51.

Putri, O. (2018). Kadar Fenolik Total dan Flavonoid Total Seduhan Daun Tin (Ficus carica) Segar dan Kering dengan Air Mendidih. JC-T (Journal Cis-Trans): Jurnal Kimia Dan Terapannya, 2(2), 7–12. https://doi.org/10.17977/um026v2i22018p007

Radwan, S., Handal, G., Rimawi, F., & Hanania, M. (2020). Seasonal Variations in Antioxidant Activity, Total Flavonoids Content, Total Phenolic Content, Antimicrobial Activity and Some Bioactive Components of Ficus carica L. in Palestine. International Journal of PharmTech Research, 13(4), 329–340. https://doi.org/10.20902/ijptr.2019.130404

Rimadhini, F. N., Sumardianto, & Romadhon. (2020). Aktivitas Antibakteri Isolat Bakteri Asam Laktat Dari Rusip Ikan Teri (Stolephorus sp.) Dengan Konsentrasi Gula Aren Cair Yang Berbeda. Jurnal Ilmu Dan Teknologi Perikanan, 2(1), 54–63. https://doi.org/10.14710/jitpi.2020.8089

Rizqiati, H., Nurwantoro, N., & Bramadita, A. M. (2023). Physicochemical, Microbiological and Organoleptic Characteristics of Rosella Water Kefir with Sucrose Sweetener. Jurnal Teknologi Hasil Pertanian, 16(2), 84. https://doi.org/10.20961/jthp.v16i2.71876

Rizqiati, H., Ramadhanti, D. L., & Prayoga, M. I. Y. (2021). Pengaruh Variasi Konsentrasi Sukrosa Terhadap Total Bakteri Asam Laktat, pH, Kadar Alkohol dan Hedonik Water Kefir Belimbing Manis (Averrhoa carambola). JURNAL ILMIAH SAINS, 21(1), 54. https://doi.org/10.35799/jis.21.1.2021.31160

Serventi, L., Cai, Y., & Sounderrajan, A. (2020). Water Kefir: A Review of its Microbiological Profile, Antioxidant Potential and Sensory Quality. Acta Scientifci Nutritional Health, 4(6), 10–17. https://doi.org/10.31080/ASNH.2020.04.0706

Setiawati, A. E., & Kusnadi, J. (2021). Optimization of fermentation time and grain concentration for water kefir production from butterfly pea flower (Clitoria ternatea). IOP Conference Series: Earth and Environmental Science, 924(1), 012081. https://doi.org/10.1088/1755-1315/924/1/012081

Triwibowo, B., Wicaksono, R., Antika, Y., Ermi, S., Jarmiati, A., Ari Setiadi, A., & Syahriar, R. (2020). The effect of kefir grain concentration and fermentation duration on characteristics of cow milk-based kefir. Journal of Physics: Conference Series, 1444(1), 012001. https://doi.org/10.1088/1742-6596/1444/1/012001

Utomo, D., & Kurniawidi, T. (2021). Pengaruh konsentrasi starter dan macam buah terhadap karakteristik kefir air. Teknologi Pangan : Media Informasi Dan Komunikasi Ilmiah Teknologi Pertanian, 12(2), 296–304. https://doi.org/10.35891/tp.v12i2.2683

Wasilu, R. P., Une, S., & Liputo, S. A. (2021). Karakteristik Kimia, Mikrobiologi, dan Organoleptik Water Kefir Sari Buah Pepaya (Carica papaya L.) Berdasarkan Lama Fermentasi dan Konsentrasi Sukrosa. Jambura Journal of Food Technology, 3(2), 13–26. https://doi.org/10.37905/jjft.v3i2.9769



DOI: https://doi.org/10.22146/agritech.82628

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