Karakterisasi Ekstrak Kurkumin dari Kunyit Putih (Kaemferia rotunda L.) dan Kunyit Kuning (Curcuma domestica Val.)

https://doi.org/10.22146/agritech.41345

Nura Malahayati(1*), Tri Wardhani Widowati(2), Anita Febrianti(3)

(1) Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32, Ogan Ilir, Sumatera Selatan 30662
(2) Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32, Ogan Ilir, Sumatera Selatan 30662
(3) Jurusan Teknologi Pertanian, Fakultas Pertanian, Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32, Ogan Ilir, Sumatera Selatan 30662
(*) Corresponding Author

Abstract


This study was aimed to identify the chemical characteristics of white and yellow turmeric powder, and to investigate the influence of both types with three different solvents on yield, total phenolic content, curcuminoid, antioxidant, and antibacterial activity of curcumin crude extract. The curcumin extraction was performed by maceration using N-hexane, ethyl acetate, and ethanol solvents. The results showed that the proximate analysis excluding carbohydrate content of white turmeric powder were lower compared to the yellow type. Curcumin crude extract of white turmeric powder had lower yield, total phenolic content, curcuminoid, antioxidant, and antibacterial activity compared to yellow turmeric extract. Moreover, turmeric extracted with ethyl acetate had higher total phenolic, curcuminoid, antioxidant, and antibacterial activity compared to turmeric extracted using N-hexane and ethanol. Based on the highest total phenolic (193.26 mg GAE/kg) and curcuminoid content (8.13 mg/L), the best treatment was yellow turmeric extract using ethyl acetate solvent. This treatment had the lowest IC50 (63.38 μg/ mL), and the highest clear zone size of S. aureus (6.59 mm) and E. coli (6.29 mm) at concentration of 2000 ppm.

Keywords


Antibacterial; chemical characteristics; curcumin crude extract; solvent; turmeric



References

Anonim. (2018). Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasn Obat Tradisional. Departemen Kesehatan RI. Jakarta.

Atlas, R.M. (1997). Principles of Microbiology. Second Edition. WNC Brown, Iowa.

AOAC. Association of Official Analytical Chemistry. (2005). Official method of analysis. 18th Edition. Association of Official Analytical Chemistry International, Washington D.C. USA.

Bagchi, A. (2012). Extraction of curcumin. IOSR Journal of Environtment Science, Toxicology and Technology (IOSR-JESTFT). 1(3): 01-16.

Boroumand, N., Samarghandian, S., & Hashemy, S.I. (2018). Immunomodulatory, anti-inflammatory, and antioxidant effects on curcumin. Journal of Herbmed Pharmacology. 7(4): 211-219.

Chinedum, E., Kate, E., Sonia, C., Ironkwe, A., & Andrew, I. (2015). Polyphenolic composition and antioxidant activities of six new turmeric (Curcuma Longa L.) accessions. Recent Pat Food Nutr Agric. 7: 22-27.

Danata, R.H., & Yamindago, A. (2014). Analisis aktivitas antibakteri ekstrak daun mangrove (Avicennia marina) dari kebupaten Trenggalek dan kabupaten Pasuruan terhadap pertumbuhan Staphylococcus aureus dan Vibrio alginolyticus. Jurnal Kelautan. 7(1).

Darsana, I.G.O., Besung, I.N.K., & Mahatmi, H. (2012). Potensi binahong (Anrederacordifolia) dalam menghambat pertumbuhan bakteri Escheria coli secara in vitro. Indonesia Jurnal Medicus Vaterrnus. 1(3): 337-351.

Deodhar, S.D., Sethi, R., & Srimal, R.C. (2013). Preliminary study onantirheumatic activity of curcumin (diferuloylmethane). Indian J.Med. Res. 138(1): 632–634.

Dewi, M.K., Evie, R., & Guntur, T. (2014). Aktivitas antibakteri ekstrak daun Majapahit (Crescentia cujete) terhadap pertumbuhan bakteri Ralstonia solanacearum penyebab penyakit layu. LenteraBio. 3(1): 51-57.

Dosoky, N.S. & Setzer, W.N. (2018). Chemical composition and biological activities of essential oils of curcuma species. Nutrients. 10(9): 1196-1238.

Dutta, B. (2015). Study of secondary metabolite constituents and curcumin contents of six different species of genus curcuma. Jurnal of Medicinal Plants Studies. 3(5): 116-119.

Ergina, Nuryanti, S., & Purpitasari, I.D. (2014). Uji kualitatif senyawa metabolit sekunder pada daun palado (Agave angustifolia) yang diekstraksi dengan pelarut air dan etanol. Jurnal Akademika Kimia. 3(3): 165-172.

Falah, S., Suzuki, T., & Kutayama, T. (2008). Chemical Constituent from Swietenia Macrophylla bark and antioxidant activity. Pakistan Journal of Biological Sciences. 11(16): 2007-2012.

Fidrianny, I., Komar, R., W., & Patricia, A.(2013). Senyawa antioksidan dari ekstrak etil asetat daun Binahong (Anredera cordifolia (Ten.) Steenis). Acta Pharmaceutica Indonesia. XXXVIII (1).

Huliselan, Y.M., Runtuwene, M.R.J., & Wewengkang, D.S. (2015). Aktivitas antioksidan ekstrak etanol, etil asetat, dan n-heksan dari daun sesewanua (Clerodendron squamatum Vahl.). Jurnal Ilmiah Farmasi-UNSRAT. 4(3): 155-163.

Istiqomah, I .(2013). Perbandingan Metode Ekstraksi Maserasi dan Sokletasi terhadap Kadar Piperin Buah Cabe Jawa (Piperis Retrofracti Fructus). UIN Jakarta. Jakarta.

Kavirayani, I.P. (2014). The chemistry of curcumin: From extraction to therapeutic agent. Molecules. 19: 20091-20112.

Khoddami, A., Wilkes, M.A., & Roberts, T.H. (2013). Techniques for analysis of plant phenolic compounds. Molecules. 18(2): 2328-2375.

Kulkarni, S.J., Maske, K.N., Budre, M.P., & Mahajan, R.P. (2012). Extraction and purificatin of curcuminoids from Tumeric (curcuma longa L.). International Journal of Pharmacology and Pharmaceutical Technology (IJPPT). 1(2): 81-84.

Listiana, A., & Herlina. (2015). Karakterisasi minuman herbal celup dengan perlakuan komposisi Jahe Merah: Kunyit Putih: dan Jahe Merah: Temulawak. Agritepa. 1(2) ISSN: 2407-1315.

Llano, S., Gómez, S., Landâno, J., & Restrepo, A. (2019). Antioxidant activity of curcuminoids. Physical Chemistry Chemical Physics. 21(7): 3752-3760.

Maizura, M., Aminah, A., & Wan Aida, W.M. (2011). Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. International Food Research Jurnal. 18: 526-531.

Pelczar, M.J., & Chan, E.S.C. (2008). Dasar-dasar Mikrobiologi 2. Jakarta: UI-Press. Terjemahan dari Element of Microbiology.

Pahaditya, D. (2012). Analisis Keragaman Genetika Tanaman Kunyit dan Temulawak secara Random Amplified Polymorphic Dna-Polymerase Chain Reaction (Rapd-Pcr) Menggunakan Primer Opa-Opd 6-10. Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor. Bogor.

Priyadarsini, K.I. (2014). The chemistry of curcumin: From extraction to therapeutic agent. Molecules. 19: 20091-20112.

Putri, R., Hasanah, R., & Kusimaningum, I. (2016). Uji aktivitas antibakteri dan uji fitokimia ekstrak daun Mangrove Sonneratia alba. Jurnal Sains dan Teknologi Akuakultur. 2 (1): 43-50.

Rahman, M., Habib, R., Hasan, R., Islam, A.M.T., & Khan, I.N. (2012). Comparative antioxidant potential of different extracts of Flacourtia Jangomas Lour Fruits. Asian Journal of Pharmaceutical and Clinical Research. 5(1):73-75.

Rahmadi, A., Puspita, Y., Nursayekti, D., Sinaga, I.S., Oktalina, R., Setiawan, H., & Murdianto, W. (2016). Analisis proksimat, senyawa fenolik, sifat antioksidan dan antibakteri kulit buah Lepisanthes alata. Jurnal Teknologi dan Industri Pangan. 27(2): 115-122.

Rosdiana, H., I. (2014). Ekstrak Kunyit Putih (Curcuma petiolata roxb.) dan Kunyit Kuning (Curcuma longa) terhadap Mortalitas Larva Anopheles sp. Program Studi Kimia Fakutas Matematika dan Ilmu pengetahuan Alam Universitas Hasanudin. Makasar.

Sepahpour, S., Selamat, J., Manap, M.Y.A., Khatib, A., & Razis, A.F.A. (2018). Comparative antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems. Molecules. 23(2): 402-419.

Septiana, A.T., Muchtadi, D., & Zakaria, F.R. (2002). Aktivitas Antioksidan Ekstrak Dikhlorometana dan Air Jahe (Zingiber offcinale Roscoe) pada Asam Linoleat. Jurnal Teknologi dan Industri Pangan. (13):2.

Tetti, M. (2014). Ekstraksi, pemisahan senyawa, dan identifikasi senyawa aktif. Jurnal Kesehatan. VII (2). ISSN 2086-2555.

Tiwari, P., Kumar, B., Kaur, G., Kaur, H., & Kaur, M. (2011). Phytochemical screening and extraction: A review. Journal International Pharmacy Science. 1: 98-106.

Wal, P., Saraswat, N., Pal, R.S., Wal, A., & Chaubey, M. (2019). A detailed insight of the anti-inflammatory effects of curcumin with the assessment of parameters, sources of ROS and associated mechanisms. Open Medicine Jurnal. 6: 64-76.

Wilken, R., Veena, M.S., Wang, M.B., & Srivatsan, E. (2011). Curcumin: A Review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma. Molecular cancer. 10(12): 1-19.

Zhang, Qing-Wen., Lin, Li-Gen., & Ye, Wen-Cai. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine. 13(20): 1-26.

Zetterström, S. (2012). Isolation and synthesis of Curcumin. Department of Physics, Chemistry and Biology Linköping University. Sweden.



DOI: https://doi.org/10.22146/agritech.41345

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