* Corresponding Author
Isolation and Purification of Oxalate Oxidase From Barley Seedling
https://doi.org/10.22146/agritech.19223 Kapti Rahayu Kuswanto(1*)
(1) Fakultas Teknologi Pertanian, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
A procedure of isolation of oxalate oxidase from barley seedling and a new method for purification is described. A purification step was accomplished by the second partition using polyethylene glycol (PEG-6000) and Devi-Iran-500 (4.5%1 4), and affinity chromatography using oxalate immobilized on activated CNBR-Sepharose 6MB as a ligand. After partition some protein were separated, and the specific activity were increased by 5-30 fold. The affinity chromatography using Oxalate-Sepharose effectively separated the oxalate oxidase. The specific activity of pure enzyme was 154.3 U/mg protein and the purity was 41 fold.
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PDF DOI: https://doi.org/10.22146/agritech.19223
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Copyright (c) 2017 Kapti Rahayu Kuswanto
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agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.