Pengaruh Penambahan NaCl sebagai Stress Agent dalam Kultivasi Sel Mikroalga Dunaliella tertiolecta ATCC 30929 terhadap Akulumasi Lipid Intrasel

https://doi.org/10.22146/agritech.13487

Karseno Karseno(1*), M. Takagi(2), T. Yoshida(3)

(1) Jurusan Teknologi Pertanian Fakultas Pertanian UNSOED Kampus Karangwangkal Purwokerto
(2) International Center for Biotecnology Osaka University, 2-1 Yamada-oka, Suita-Shi Osaka, Japan
(3) International Center for Biotecnology Osaka University, 2-1 Yamada-oka, Suita-Shi Osaka, Japan
(*) Corresponding Author

Abstract


Lipid composition of microalgae cell was affected by saline medium. Increasing of initial NaCI in modified NORO medium from 0.5 M to 1.0 M resulted in an increase of intracellular lipid content of Dunaliella tertiolecta ATCC 30929 cell from 60% to 67% (db). Loading of 0.5 M NaCI at a middle of logarithmic phase or 1.0 M at the end of logarithmic phase during the growth on the same medium increased intracellular lipid content to 70% (db). Addition of NaCI up to 2.0 M during the culture gave a high intracellular lipid content up to 77% (db) , although cell concentration was decreased to a half The temperature, light intensity, and aeration was adjusted at 30 °C, 150 mo1.11.m-2 (10.000 lux) and 250 ml/min CO2 enriched air (CO2 3%).

Keywords


Dunaliella; microalgae; intracellular NaCI

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DOI: https://doi.org/10.22146/agritech.13487

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Agritech (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.


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