Pemanfaatan Fraksi Kaya Asam Laurat Hasil Hidrolisis dari Endosperm Kelapa Menggunakan Lipase Endogeneus Sebagai Pengawet Susu Kedelai Kemasan

https://doi.org/10.22146/agritech.12859

Moh. Su'i(1*), Enny Sumaryati(2), Dani Dwi Sucahyono(3)

(1) Fakultas Pertanian, Universitas Widyagama Malang, Jl. Borobudur No.25, Malang 65128
(2) Fakultas Pertanian, Universitas Widyagama Malang, Jl. Borobudur No.25, Malang 65128
(3) Fakultas Pertanian, Universitas Widyagama Malang, Jl. Borobudur No.25, Malang 6512
(*) Corresponding Author

Abstract


Results of previous studies show that the high lauric fraction isolated from coconut endosperm is able to inhibit pathogenic and non-pathogenic bacteria. This research aims to study the addition of high lauric fraction that hydrolysed of coconut endosperm of the storability of soy milk packaging. High lauric fraction isolated from coconut milk, then the fraction analized of the fatty acid composition with gas chromatography (GC) and then used as a preservative soy milk. The fraction is added to the soy milk with concentrations of 0, 10, 15 and 20%, then stored for 3 days. Every day is observed until soy milk damaged. The results showed that the fraction isolated from coconut milk contains 50.45% lauric acid, 17.52% myristic acid, 7.02% palmitic acid, 6.46% capric acid, 5.52% caprylic acid, 5.12% linoleic acid, 1.89% oleic acid, and 0.11% caproic acid. The addition of lauric acid-rich fraction of 20% were able to preserve soy milk for 2 days with a total microbe 1.00 x 104 cfu/ml, free fatty acids 0.12 m mol/ml, pH 5.05 and a balanced aroma 4 (nice).

ABSTRAK

Hasil penelitian sebelumnya menunjukkan bahwa fraksi kaya asam laurat hasil isolasi dari endosperm kelapa mampu menghambat bakteri patogen dan non patogen. Penelitian ini bertujuan mempelajari penambahan fraksi kaya asam laurat hasil hidrolisis dari endosperm kelapa terhadap daya simpan susu kedelai kemasan. Fraksi yang kaya asam laurat diisolasi dari santan kelapa kemudian fraksi tersebut diuji komposisi asam lemaknya menggunakan chromatografi gas (GC) dan selanjutnya digunakan sebagai bahan pengawet susu kedelai. Fraksi kaya asam laurat ditambahkan ke dalam susu kedelai dengan konsentrasi 0, 10, 15 dan 20%, kemudian disimpan selama 3 hari. Setiap hari dilakukan pengamatan hingga susu mengalami kerusakan. Hasil penelitian menunjukkan bahwa fraksi hasil isolasi dari santan kelapa mengandung asam laurat 50,45%, asam miristat 17,52%, asam palmitat 7,02%, asam kaprat 6,46%, asam kaprilat 5,52%, asam linoleat 5,12%, asam oleat 1,89% dan asam kaproat 0,11%. Penambahan fraksi kaya asam laurat sebesar 20% mampu mengawetkan susu kedelai selama 2 hari dengan total mikroba 1,00 x 104 cfu/ml, asam lemak bebas 0,12 m mol/ml, pH 5,05 dan skor aroma 4 (menyukai).


Keywords


Coconut; lauric acid; soy milk; storage

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DOI: https://doi.org/10.22146/agritech.12859

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Copyright (c) 2016 Moh. Su'i, Enny Sumaryati, Dani Dwi Sucahyono

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agriTECH (print ISSN 0216-0455; online ISSN 2527-3825) is published by Faculty of Agricultural Technology, Universitas Gadjah Mada in colaboration with Indonesian Association of Food Technologies.


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