Bioavailabilitas α-Tokoferol Minuman Emulsi Minyak Sawit dalam Plasma Darah dan Hati Tikus (Rattus norvegicus)

https://doi.org/10.22146/agritech.11683

Winda Christina Harlen(1*), Tien Ruspriatin Muchtadi(2), Nurheni Sri Palupi(3)

(1) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16002
(2) Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16002
(3) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16002
(*) Corresponding Author

Abstract


Theoritically, it should contains high amount of α-tocopherol but its bioavailability have not been studied yet. The aim of this research were (1) to review the chemical composition of POED by doing proximate test then calculate α-tocopherol level in the product and (2) to evaluate bioavailability of α-tocopherol based on in vivo study. Bioavailability evaluation was carried out by measuring Relative Tocopherol Accumulation Factor (TAF), meanwhile its chemical composition was measured by proximate test and chromatographic analysis using HPLC. α-tocopherol contents of this products were 192,63 μg/g. Bioavailability study was carried out for 14 days using Rattus norvegicus rats. The prepared food contains 30 mg/kg α-tocopherol were given to vitamin-E-depleted rats. Tocopherol Accumulation Factor (TAF) proposed for the test group is 1/45,61, which indicated that 45,61 μg vitamin E from this product was needed to accumulate 1 μg tocopherol in the liver. Tocopherol relative bioavailability of POED was 82,46%. It showed that POED has good bioavailability.

ABSTRAK

Minuman emulsi minyak sawit (MEMS) merupakan salah produk berbasis emulsi oil-in-water yang dapat menjadi sumber vitamin E dalam bentuk α-tokoferol. Komposisi dasarnya adalah olein minyak sawit, air, dan pengemulsi. Secara teoritis, produk ini kaya akan α-tokoferol namun bioavailabilitasnya belum diketahui. Oleh karena itu, tujuan penelitian ini adalah (1) Menganalisis komposisi kimia MEMS melalui uji proksimat dan kadar α-tokoferolnya, dan (2) Mengevaluasi bagaimana bioavailabilitas α-tokoferol secara in vivo. Evaluasi bioavailabilitas dengan memperhitungkan Tocopherol Accumulation Factor (TAF) relatif, sedangkan komposisi kimia didapatkan melalui analisis proksimat dan kromatografi dengan HPLC untuk mengetahui kadar α-tokoferol. Kadar α-tokoferol yang didapatkan sebesar 192,63 μg/g. Pengujian biovailabilitas dilakukan selama 14 hari menggunakan hewan coba berupa tikus Rattus norvegicus. Ransum yang diberikan mengandung 30 mg/kg α-tokoferol. Nilai TAF sebesar 1/45,61, yang berarti bahwa 45,61 μg vitamin E dari MEMS dapat menghasilkan 1 μg tokoferol hati. Bioavailabilitas relatif α-tokoferol pada MEMS terhadap α-tokoferol murni sebesar 82,46%. Hasil tersebut menunjukkan bahwa MEMS memiliki bioavailabilitas yang baik.

Kata kunci: α-tokoferol; bioavailabilitas; minyak sawit; minuman emulsi minyak sawit; Tocopherol Accumulation Factor


Keywords


α-Tocopherol; bioavailability; palm oil; palm oil emulsion drink; Tocopherol Accumulation Factor

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References

Association of Official Analytical Chemist (AOAC). (2005). Official Methods of Analysis (18th edition). Association of Official Analytical Chemistry. Published by the Association of Official Analytical Chemist. New York, USA.

Association of Official Analytical Chemist (AOAC). (2012). Official Methods of Analysis (19th edition). Association of Official Analytical Chemistry. Published by the Association of Official Analytical Chemist. New York, USA.

Badan Standarisasi Nasional (BSN). (2006). Standar Nasional Indonesia (SNI) nomor 01-2354.3-2006. Published by Badan Standarisasi Nasional. Jakarta, Indonesia

Ball, G.F.M. (2006). Vitamins in Foods: Analysis, Bioavailability, and Stability. Taylor and Francis Group, Boca Raton

Bella, D.L., Schock, B.C., Lim, Y., Leonard, S.W., Berry, C., Cross, C.E., Traber, M.G. (2006). Regulation of the alpha-tocopherol transfer protein in mice: lack of response to dietary vitamin E or oxidative stress. Lipids 41, 105–12.

Borel, P., Preveraud, D., Desmarchelier, C. (2013). Bioavailability of vitamin E in humans: An update. Nutrition Review 71, 319–331.

Carrillo-Lopez, A., Yahia, E.M., Ramirez-Padilla, G.K. (2010). Bioconversion of carotenoids in five fruits and vegetables to vitamin a measured by retinol accumulation in rat livers. American Journal of Agricultural and Biological Sciences 5(2): 215–221.

Devaraj, S., Tang, R., Adams-Huet, B., Harris, A., Seenivasan, T., de Lemos, J.A., Jialal, I., (2007). Effect of high-dose alpha-tocopherol supplementation on biomarkers of oxidative stress and inflammation and carotid atherosclerosis in patients with coronary artery disease. American Journal of Clinical Nutrition 86(5): 1392–1398.

Domitrovic, R., Tota, M., Milin, C. (2008). Differential Effect of High Dietary Iron on α-Tocopherol and Retinol Levels in The Liver and Serum of Mice Fed Olive Oil-and Corn Oil-Enriched Diets. Nutrition Research 28(4): 263–269.

Ernst, I.M.A., Pallauf, K., Bendall, J.K., Paulsen, L., Nikolai, S., Huebbe, P., Roeder, T., Rimbach, G. (2013). Vitamin E supplementation and lifespan in model organisms. Aging Research Review 12: 365–375.

Fahami, N.A.M, Ismail, N.M, Khalid, K.B.A. (2005). Effects of Vitamin E on Food Intake and Body Weight in Rats Exposed to Restraints Stress. Medical Journal of Islamic World Academy of Science 15(2): 81–86.

Fernández-García, E., Carvajal-Lérida, I., Jarén-Galán, M., Garrido-Fernández, J., Pérez-Gálvez, A., Hornero-Méndez, D. (2012). Carotenoids bioavailability from foods: From plant pigments to efficient biological activities. Food Research International 46, 438–450.

Gagne, A., Wei, S.Q., Fraser, W.D., Julien, P. (2009). Absorption, Transport, and Bioavailability of Vitamin E and its Role in Pregnant Women. Journal of Obstetetry and Gynaecology Canada 31, 210–217.

Gee, P.T. (2011). Unleashing the untold and misunderstood observations on vitamin e. Genes Nutrition 6, 5–16.

Grebenstein, N., Frank, J. (2012). Rapid baseline-separation of all eight tocopherols and tocotrienols by reversed-phase liquid-chromatography with a solid-core pentafluorophenyl column and their sensitive quantification in plasma and liver. Journal of Chromatography A 1243: 39–46.

Hatanaka, J., Chikamori, H., Sato, H., Uchida, S., Debari, K., Onoue, S., Yamada, S. (2010). Physicochemical and pharmacological characterization of α-tocopherol-loaded nano-emulsion system. International Journal of Pharmacheutics 396, 188–193.

Li, C., Li, R.W., Elsasser, T.H. (2010). Gene Regulation and Systems Biology Alpha-Tocopherol Modulates Transcriptional Activities that Affect Essential Biological Processes in Bovine Cells Gene Regulation and System Biology 4: 109–124

Lim, Y., Traber, M.G. (2007). Alpha-Tocopherol Transfer Protein (α-TTP): Insights from Alpha-Tocopherol Transfer Protein Knockout Mice. Nutrition Research and Practice 1, 247–253.

Mancini, A., Imperlini, E., Nigro, E., Montagnese, C., Daniele, A., Orru, S., Buono, P. (2015). Biological and nutritional properties of palm oil and palmitic acid: Effects on health. Molecules 20, 17339–17361.

McClements, D.J., Saliva-Trujillo, L., Zhang, R., Zhang, Z., Zou, L., Yao, M., Xiao, H. (2016). Boosting the bioavailability of hydrophobic nutrients, vitamins, and nutraceuticals in natural products using excipient emulsions. Food Research International 88, 140–152.

Meridian. Y.A. (2000). Kajian Ketersediaan Hayati β-Karoten Minuman Emulsi Karoten Minyak Sawit dalam Hati dan Plasma Tikus (Rattus norvegicus). Skripsi. Fakultas Teknologi Pertanian. Institut Pertanian Bogor.

Parada, J., Aguilera, J.M. (2007). Food microstructure affects the bioavailability of several nutrients. Journal of Food Science 72, 21–32.

Sogut, E., Kad, H., Karayakal, M., Mertoglu, C. (2015). The association of plasma vitamin A and E levels with coronary collateral circulation. Atherosclerosis 239, 547–551.

Surfiana. (2002). Formulasi Minuman Emulsi Kaya β-karoten dari Minyak Sawit Merah. Tesis. Fakultas Teknologi Pertanian. Institut Pertanian Bogor.

Takeuchi, H., Sekine, S., Kojima, K., Aoyama, T. (2008). The application of medium-chain fatty acids: edible oil with a suppressing effect on body fat accumulation 382. Asia Pacific Journal of Clinical Nutrition 17(1), 320–323.

Yang, Y., McClements, D.J. (2013). Vitamin E bioaccessibility: Influence of carrier oil type on digestion and release of emulsified α-tocopherol acetate. Food Chemistry 141, 473–481.



DOI: https://doi.org/10.22146/agritech.11683

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