Analysis of Adulterated Pangasius Hypopthalmus Oil by ATR-FTIR Spectroscopy and Chemometric

Abstract

In this recent study, ATR-FTIR spectroscopy and chemometrics have been successfully used for the
classification and quantitative analysis of adulterated Pangasius Hypopthalmus (P. hypopthalmus) oil. The aim
of this research was to evaluate the ability of ATR-FTIR spectroscopy and chemometric to perform the
classification and quantitative analysis of adulterated P. hypopthalmus oil in a binary mixture with palm oil
(PO) and coconut oil (CO). In the development of FTIR spectroscopy combined with chemometrics for the
classification and quantitative analysis of P. hypopthalmus oil, P. hypopthalmus oil (MP and LFP) mixed with
other oils such as coconut oil (CO) and palm oil (PO) at concentrations of 1-99% v/v. Classification of P.
hypopthalmus oil, PO and CO were performed by the principal component analysis (PCA) and the
quantification analysis was carried out by partial least square (PLS). Based on the optimization process, the
best classification results were obtained using the first derivative spectra at wave numbers of 1400-1100 cm-1.
The prediction of percentage adulterated oil by PLS method also showed very good values of R2 greater than
0.9999 and low standard error values in the range of 0.0176-0.703. The prediction was also perform at
1400-1100 cm-1 wavenumbers using the first derivative spectra.