Journal of Food and Pharmaceutical Sciences

Optimization of Carbopol, CMN-Na, Gelatin, and In Vitro Activity Test of 4-Hydroxy Chalcone Gel as Sunscreen

Abdul Karim Zulkarnain, Jumina Jumina — Thu, 08 Jun 2023 18:45:46 +0700

Abstract: The 4-hydroxychalcone compound is a flavonoid derivative that has benzene rings with unsaturated chains and a chromophore group, which can absorb UV rays and reduce the intensity of radiation exposure to the skin. This study aims to examine the effect of gelling agents on the physical properties of gel preparations, optimum formula stability, and activity of 4-hydroxychalcone gel as a sunscreen in vitro. Base optimization was carried out using Design Expert software version 10 with the simplex lattice design method and variations in gelling agent concentrations, namely carbopol, sodium carboxymethylcellulose (CMC-Na), and gelatin. The activity of 4-hydroxychalcone sunscreen gel was determined spectrophotometrically by determining the Sun Protecting Factor (SPF) value, the percentage of erythema transmission (%TE), and the percentage of pigmentation transmission (%TP). Data were analyzed statistically with SPSS software. The results showed that the optimum formula composition was obtained at a concentration of 0.67% carbopol, 2% CMC-Na, and 1.83% gelatin. The optimum formula of 0.5% 4-hydroxychalcone gel was stable during storage at room temperature and did not experience syneresis during 72 hours of storage at ± 10°C. The 0.5% 4-hydroxychalcone gel gave an SPF value of 27.37 % TE was 1.76% and % TP was 10.21%, therefore it is categorized as a sunblock.

Optimization of HPMC and Na-CMC as Gelling Agents on Physical Properties and Stability in Sunflower Seed Oil Gel Formulation

Sanubari Indah Putri Rahmani , Abdul Karim Zulkarnain — Thu, 08 Jun 2023 18:46:14 +0700

Abstract: In recent years, skincare with natural ingredients is being developed for it has fewer side effects and is relatively suitable for all skin types. One of the potential ingredients from the natural ingredients is sunflower seed oil, which has proven to have potential as a skin moisturizer and fight acne bacteria. The purpose of this study was to make a gel formulation of sunflower seed oil by optimizing the combination of the gelling agent of HPMC and Na-CMC. The Simplex Lattice Design method was used to optimize the gel base with Design Expert software version 13. There were 8 gel formulas made and evaluated including organoleptic, homogeneity, pH, viscosity, spreadability, and adhesion. The independent variables in SLD were the amount of HPMC and Na-CMC, while the responses included pH, viscosity, and spreadability. The optimal formula suggested by SLD is a combination of 1.451% HPMC and 1.549% Na-CMC. The optimal formula has a pH of 6.813 ± 0.041, a viscosity of 194.8 ± 2.94 dPas, an adhesion of 6.29 ± 0.50 seconds, and a spreadability of 21.5 ± 10.05 cm2. The optimum prediction formula from the software has no significant difference from the test results which show that the method can be used to predict the physical properties of sunflower seed oil gel preparations. The results of the physical stability test also stated that its physical properties were stable in terms of pH, viscosity, spreadability, and adhesion.

Intrinsic Factors Contributing to Shrimp and Blood Clamp Allergic Reaction and Allergic Like Symptoms

Almasah Azzahra, Bramanti Nadya Kausara, Muhammad Novrizal Abdi Sahid — Thu, 22 Jun 2023 22:22:06 +0700

Factors that contribute to food allergenicity is still unknown. To determine food intrinsic properties that contribute to L. vannamei and A. granosa allergenicity or allergy like symptoms this study identify total protein and metal profiles, histamine contents, and protein heat stability. Histamine content is measured colorimetrically. Protein heat-stability is observed with sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE). Allergy-related metal concentration are determined with Atomic Absorption Spectroscopy (AAS). Fresh L. vannamei and A. granosa contained ~100 and ~25 ppm histamine, respectively. Storage at -20 ^oC for 3 days relatively did not changed histamine content of both foods. Storage at 4-8 ^oC for 3 days increased histamine content in L. vannamei, but not in A. granosa. Protein sized <70 kDa of both samples are relatively heat-stable. L. vannamei and A. granosa contained Zn with concentration of 85 ppm and 92 ppm, subsequently. All samples did not contain nickel (Ni), chromium (Cr), and lead (Pb). Higher histamine content and heat-stable protein number of L. vannamei could contribute to higher prevalence of its allergy or allergic like symptoms than A. granosa. High Zn content might contribute to allergic like symptoms of both foods.

In silico Pharmacokinetic and Toxicity Prediction of Compounds from Andrographis paniculata (Burm.f.) Nees.

Izatunnafis Izatunnafis, Yosi Bayu Murti, Bambang Sulistyo Ari Sudarmanto — Thu, 22 Jun 2023 22:23:26 +0700

Many compounds have been isolated from Andrographis paniculata (Burm. f.) Nees (AP). In drug discovery and development, plant secondary metabolites are popular as resources for drug candidates. A high-quality drug candidate should not only be effective against the therapeutic target, but it should also be safe and have good pharmacokinetic features. This study aimed to predict the pharmacokinetic features and toxicity potencies of 46 compounds from AP using the pKCSM online tool. According to pKCSM prediction, among the forty-six compounds from AP, compound 1 (14-Deoxy-11,12-didehydroandrographolide), compound 2 (14-Deoxyandrographolide), and compound 39 ((-)-beta-Sitosterol) have good pharmacokinetic features and do not have potencies to be mutagenic and hepatotoxic agents. The lethal dosage values (LD₅₀) of compounds 1, 2, and 39, are 1935, 2053, and 2424 (mol/kg), respectively. However, further research is still needed to confirm these predictions.

The Allergenicity Potential of Edible Freshwater Fish

Atika Hanum Falihah, Muhammad Novrizal Abdi Sahid, Ika Puspitasari — Tue, 11 Jul 2023 14:50:24 +0700

A Freshwater fish is a popular type of fish consumed in Indonesia besides seawater fish. However, until now the most available information regarding the potential allergenicity of fish is only related to seawater fish. The potential allergenicity of freshwater fish has not been studied much, so it has not been identified and characterized. Based on this, this review aims to collect information regarding the potential allergenicity of protein in freshwater fish and the effect of processing on this potential. The potential allergenicity of proteins in several freshwater fish has been confirmed by immunoblotting and ELISA, namely mujair, nile, catfish, mas, toman, janjan, tambakan, tiger scat, barramundi, and eel. The identified allergenic proteins include parvalbumin, tropomyosin, enolase, aldolase, creatine kinase, triosephosphate isomerase, pyruvate kinase, lactate dehydrogenase, glucose-6-phosphate isomerase, and glyceraldehyde-3-phosphate dehydrogenase. In addition, several proteins in other freshwater fish also have allergenicity potential but have not been confirmed through research, namely pomfret, gourami, snakehead, catfish, eel, betutu, and cetol. The potential allergenicity of fish protein is also influenced by the processing process. Changes in the potential allergenicity are related to the characteristics of each allergen protein. Therefore, it is necessary to select the appropriate processing so that potential allergenicity can be suppressed.

Comparison of Xenograft and Alloplast Bone Grafts for Infrabony Bone Defect: Literature Review

Hesty Nurcahyanti, Martina Amalia — Tue, 11 Jul 2023 14:55:21 +0700

Restoring supporting tissues to a healthy state is a critical area that provides a much more appealing beside prevention, initial therapy, and corrective procedure. Infrabony defects can be found in the interalveolar and marginal bone caused by periodontal disease as a result of local factors. Nowadays, various surgical therapies are available for treating infrabony defects such as bone graft. However, many considerations in selecting the best option of bone graft materials. To compare the effectiveness of Xenograft and Alloplast bonegraft in infrabony defect therapy. The allograft presented a significantly higher amount of newly formed bone than the xenograft. There were no differences in the percentage of newly formed bone between the allograft and the alloplastic graft, and between the alloplastic graft and the xenograft. Between the three types of grafts, there were no discernible changes in the percentage of remaining particles. All bone substitute materials shown favorable properties for use in bone regeneration treatments. Local factors such as oral hygiene and controlled chronic disease are the main point of successful bone graft therapy. The present study concludes that both Alloplast and Xenograft have equal potential in infrabony bone defect therapy.

Physicochemical Characterization and Fatty Acid Profiles of Catfish Oil (Clarias gariepinus)

Karina Primatyas Ningrum, Abdul Rohman, Ronny Martien — Sun, 16 Jul 2023 11:00:47 +0700

Indonesian aquaculture cultivation production of catfish has been growing faster than other fish. It is a potential source of fish oil and contains polyunsaturated fatty acids that have health benefits. The objective of this study was to perform physicochemical characterization and fatty acid profile of catfish oil. This study used fish oil extracted from the head and the flesh of catfish using dry rendering with a mechanic press. The result showed that catfish flesh oil (CFO) and catfish head oil (CHO) were significantly different parameters (p<0.05) in terms of physicochemical characteristics including acid value, peroxide value, iodine value, and saponification value. The best characteristics are catfish flesh oil than catfish head oil for acid value, peroxide value, iodine value, and saponification value following the number is 2.17 mg KOH/g, 5.15 meqO2/kg, 76.13 I₂/100 g, 175.86 mg KOH/g. The predominant fatty acids were oleic, palmitic, and linoleic acids. These catfish oils are found suitable for consumption for beneficial health effects.