Total Flavonoid Content and Antioxidant Activity of Temu Mangga (Curcuma mangga Val. & Zijp) and its Classification with Chemometrics

Abstract

Indonesia is a rich country in terms of its biodiversity. Herbs that are widely used to
maintain a healthy body comes from the Zingiberaceae family, including temu mangga. Temu mangga
(Curcuma mangga Val. & Zijp) is effective for relieving gastric pain, diarrhea, menstrual pain, acne
and ulcers, and increasing appetite. Besides, temu mangga is considered to have antioxidant activity
due to the presence of the phenolic and flavonoid compounds. This study aims to determine the
correlation and classification of temu mangga from various places based on total flavonoid content
and antioxidant activities. The samples were extracted with methanol, then tested for total
flavonoid content by AlCl3 method and antioxidant activity by DPPH free radical capture method.
Analysis was performed using correlation coefficient value and chemometrics PCA (Principal
Component Analysis) and CA (Cluster Analysis) to see the relationship between total flavonoid
content with their antioxidant activity and to see their classification. Total flavonoid content of
methanolic extract of temu mangga gives a moderate correlation to its antioxidant activity with r =
-0.6085 and clustering samples successfully determined by PCA and CA which resulted in 4 groups.
Keywords: Curcuma mangga Val. & Zijp, Methanolic Extract, Flavonoid, Antioxidant, PCA, CA