Comparison of Anti-oxidant Activity of Five Elected Wild Commercial Mushrooms in Four Different Solvents as an Alternative Oxidative Stress Reliever

Abstract

Mushroom also known as a lit source of nutritional and therapeutical component so in order to
evaluate the usefulness of the Pakistani wild mushroom strains as the possible nutracueticals, adetailed
study entailing several tests for the determination of anti-oxidant potential was carried out on the elected
mushrooms in four different solvents. Strains of Lentinus edodes, Pleurotus ostreatus, Volvariella volvacea,
Pleurotus eryngii and Ganoderma lucidum were extracted for this particular purpose in the water, n- butanol,
n-hexane and choloroform solvents. Test models including Total Phenolic Content assay, Total Flavonoid
Content assay, 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) Scavenging Activity assay and Reducing Power
assay were utilized for carrying out the comparative analysis among the selected mushrooms. The acquired
results indicated that the extract of Volvariella volvacea and Pleurotus eryngii possess copacetic anti-oxidant
potential and can be utilized as oxidative stress reliever in humans. Furthermore, extraction studies also
indicated that water is the most suitable solvent for anti-oxidants extraction for these mushrooms. Finding of
the anti-oxidant potential for elected wild mushrooms contra indicate that these mushrooms can be safely
exploited as a amended natural anti-oxidant in comparison to its synthetic counterpart.