Changes of Physicochemical and Microbiological Properties of Blended Sweet and Sour Pomegranate Juices During Refrigerated Storage
Faten Boussaa(1*), Faten Zaouay(2), Messaoud Mars(3)
(1) Research Unit on Agrobiodiversity (UR13AGR05), Higher Agronomic Institute, Chott-Mariem
(2) Research Unit on Agrobiodiversity (UR13AGR05), Higher Agronomic Institute, Chott-Mariem
(3) Research Unit on Agrobiodiversity (UR13AGR05), Higher Agronomic Institute, Chott-Mariem
(*) Corresponding Author
Abstract
Physicochemical and microbiological properties changes of new beverages combining sour (‘Garoussi 2’, ‘Mezzi 1’, ‘Mezzi 2’) and sweet (‘Gabsi’, ‘Tounsi’) pomegranate juices with different proportions (10% and 20% of sour juices) were studied over a 14 days refrigerated storage. The sour juices have proved to be very rich in total soluble solids, total anthocyanin and total phenolic contents. However, these juices are not appreciated by panellists because of high acidity content. The juice blends were characterized by an attractive red colour, acceptable acidity, higher total soluble solids content, higher total phenol and anthocyanin contents compared to sweet juices. The juice blending was found to be very effective in reducing the browning and improving the appearance of beverages during storage. High microbial loads of juice samples stored up to 14 days indicate their poor microbiological quality. The beverage based on Gabsi and Garoussi 2 (10 % and 20 % proportions) presented better nutritional and microbiological stability during storage leading to their high consumer acceptance. It was concluded that conservation of juice samples should not exceed 7 days in order to have healthy value-added beverages.
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Addo, M.G., Akanwariwiak, W.G., Addo-Fordjour, P. and Obiri-Danso, K. 2008. Microbiological and Sensory Analysis of Imported Fruit Juices in Kumasi, Ghana. Research Journal of Microbiology. 3(8): 552-558. doi: 10. 3923/jm.2008.552.558.
Alighourchi, H and Barzegar, M. 2008. Anthocyanins characterization of 15 Iranian pomegranate (Punica granatum L.) varieties and their variation after cold storage and pasteurization. European Food Research and Technology. 227: 881–887. doi: 10.1007/s00217-007-0799-1.
Arnous, A., Makris, D.P. and Kefalas, P. 2001. Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. Journal of Agriculture of Food Chemistry. 49: 5736-5742. doi: org/10.1021/jf010827s.
Batra, N.G., Sharma, A. and Agarwal, N. 2018. Evaluation of microbiological criteria and quality of packed fruit juices. International Food Research Journal. 25(2): 458-461.
Bhardwaj, R.L and Mukherjee, S. 2011. Effects of fruit juice blending ratios on kinnow juice preservation at ambient storage condition. African Journal of Food Science. 5(5): 281 – 286.
Carbonell-Barrachina, A.A., Calin-Sanchez, A., Bagatar, B., Hernandez, F., Legua, P., Martınez-Font, R. and Melgarejo, P. 2012. Potential of Spanish sour–sweet pomegranates (cultivar C25) for the juice industry. Food Science and Technology International. 18(2): 129-138. doi.org/10.1177/1082013211414783.
Cheng, G.W and Breen, B.J. 1991. Activity of phenylalanyl ammonialyase (PAL) and concentrations of anthocyanins and phenolics in developping strawberry fruit. Journal of the American Society for Horticultural Science. 116: 865-868. doi.org/10.21273/JASHS.116.5.865.
Coutinho, I.B., Freitas, A., Maçanita, A.L. and Lima, J.C. 2015. Effect of water content on the acid–base equilibrium of cyanidin-3-glucoside. Food Chemistry. 172 : 476–480. doi.org/10.1016/j.foodchem.2014.09.060.
De Corrêa Souza, M., Cristina, M., Benassi, M. D. T. and De Almeida, R. F. 2004. Stability of unpasteurized and refrigerated orange juice. Brazilian Archives of Biology and Technology. 47: 391–397. doi. 10.1590/S1516-89132004000300009.
Galani, J.HY., Patel, J.S., , Patel, N.J. and Talati, J.Y. 2017. Storage of Fruits and Vegetables in Refrigerator Increases their Phenolic Acids but Decreases the Total Phenolics, Anthocyanins and Vitamin C with Subsequent Loss of their Antioxidant Capacity. Antioxidants. 6: 59. doi. 10.3390/antiox6030059.
Geta, K., Kebede, A. and Chemedissa, M. 2018. Microbiological Safety of Fruit Juices Consumed in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia. Preprints, 2018060027. doi. 10.20944/preprints201806.0027.v1.
Gonzalez-Molina, E., Moreno, D.A. and Garcia-Viguera, C. 2009. A new drink rich in healthy bioactives combining lemon and pomegranate juices. Food Chemistry. 115 : 1364-1372. doi.org/10.1016/j.foodchem.2009.01.056.
Gulf Standards. 2000. Microbiological criteria for food stuffs-part 1. GCC, Riyadh, Saudi Arabia, 7-20.
Hasnaoui, N., Mars, M., Ghaffari, S., Trifi, M., Melgarejo, P. and Hernandez, H. 2011. Seed and juice characterization of pomegranate fruits grown in Tunisia: Comparison between sour and sweet cultivars revealed interesting properties for prospective industrial applications. Industrial Crops and Products. 33: 374–381. doi.org/10.1016/j.indcrop.2010.11.006.
Hossain, M. Shishir, M., Saifullah, M.R.I., Sarker, M., Safeuzzaman, K.U. and Rahman, M. A. 2016. Production and Investigation of Biochemical and Organoleptic Changes of Mixed Fruit Juice during Storage Period. - Indian Journal of Nutrition Diet. 3(1): 119- 126.
Kalt, W and McDonald, J.E. 1996. Chemical Composition of Lowbush Blueberry Cultivars. Journal of the American Society for Horticultural Science. 121(1): 142-146. doi.org/10.21273/JASHS.121.1.142.
Karpagavalli, B and Amutha, S. 2015. Influence of storage condition on the antioxidant activity of pomegranate squash. Plant Archives. 15(1): 405-410.
Katsumoto, Y., Fukuchi-Mizutani, M., Fukui, Y., Brugliera, F., Holton, T.A., Karan, M., Nakamura, N., Yonekura-Sakakibara, K., Togami, J., Pigeaire, A., Tao, G.Q., Nehra, N.S., Lu, C.Y., Dyson, B.K., Tsuda, S., Ashikari, T., Kusumi, T., Mason, J.G. and Tanaka, Y.2007. Engineering of the rose flavonoid biosynthetic pathway successfully generated blue-hued flowers accumulating delphinidin. Plant Cell Physiology. 48(11): 1589–1600. doi.org/10.1093/pcp/pcm131.
Khoo H.E., Azlan, A., Tang, S.T. and Lim, S.M. 2017. Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutrition and Research. 61(1): 1361779. 10.1080/16546628.2017.1361779.
Lo Piero, A. R., Puglisi, I., Rapisarda, P. and Petrone, G. 2005. Anthocyanins Accumulation and Related Gene Expression in Red Orange Fruit Induced by Low Temperature Storage. Journal of Agricultural and Food Chemistry. 53(23): 9083–9088. 10.1021/jf051609s.
Mars, M. 2001. Genetic resources of pomegranate (Punica granatum L.) in Tunisia: Prospection, conservation and diversity analysis. Ph.D. Thesis in Natural Sciences. Tunis El Manar University, pp. 200.
Muzaffar, M., Nayik, G.A., Gull, A. and Kumar, P. 2017. Changes in quality characteristics of pomegranate juice concentrate during refrigerated storage. Journal of Postharvest and Technology. 5(3):16-21.
Nayik, G.H., Amin, T. and Bhat, S.V. 2014. Microbial analysis of some fruit juices available in the markets of Kashmir valley, India. Asian Journal of Microbioly Biotechnology and Environmental Science. 15(4): 733-737.
Pontonio, E., Montemurro, M., Pinto, D., Marzani, B., Trani, A., Ferrara, G., Mazzeo, A., Gobbetti, M. and Rizzello, C.G. 2019. Lactic Acid Fermentation of Pomegranate Juice as a Tool to Improve Antioxidant Activity. Frontiers in Microbiology. 10 : 1550. 10.3389/fmicb.2019.01550.
Rommel, A., Heatherbell, D.A. and Wrolstad, R.E. 1990. Red Raspberry Juice and Wine: Effect of Processing and Storage on Anthocyanin Pigment Composition, Color and Appearance. Food Science. 55(4): 1011-1017. 10.1111/j.1365-2621.1990.tb01586.x.
Seeram, N.P., Aviram, M., Zhang, Y., Henning, S.M., Feng, L., Dreher, M. and Heber, D. 2008. Comparison of Antioxidant Potency of Commonly Consumed Polyphenol-Rich Beverages in the United States. Journal of Agriculture Food and Chemistry. 56: 1415–1422. 10.1021/jf073035s.
Suárez-Jacobo, Á., Gervilla, R., Guamis, B., Roig-Sagués, A.X. and Saldo, J. 2010. Effect of UHPH on indigenous microbiota of apple juice. A preliminary study of microbial shelf-life. International Journal of Food Microbiology. 136: 261–267. doi; 10.1016/j.ijfoodmicro.2009.11.011.
Tribst, A.A.L., Sant’Ana, ADS. and de Massaguer, P.R. 2009. Review: Microbiological quality and safety of fruit juices—past, present and future perspectives. Critical Reviews in Microbiology. 35(4),: 310-339. doi. 10.3109/10408410903241428.
Vardin, H. and Fenercioglu, H. 2003. Study on the development of pomegranate juice processing technology: clarification of pomegranate juice. Nahrung 42: 300-303. doi.org/10.1002/food.200390070.
Varela-Santos, E., Ochoa-Martinez, A., Tabilo-Munizaga, G., Reyes, J.E., Pérez-Won, M., Briones-Labarca, V. and Morales-Castro, J. 2012. Effect of high hydrostatic pressure (HHP) processing on physicochemical properties, bioactive compounds and shelf-life of pomegranate juice. Innovative Food Science and Emerging Technologies. 13: 13–22. doi.org/10.1016/j.ifset.2011.10.009.
V´azquez-Ara´ujo, L., Chambers, IV E., Adhikari, K. and Carbonell-Barrachina, ´A.A. 2010. Sensory and Physicochemical Characterization of Juices Made with Pomegranate and Blueberries, Blackberries, or Raspberries. Journal of Food Science. 75 (7): 398-404. doi.org/10.1111/j.1750-3841.2010.01779.x.
Vitti, M.C.D., Sasaki, F.F., Miguel, P., Kluge, R.A. and Moretti, C.L. 2011. Activity of Enzymes Associated with the Enzymatic Browning of Minimally Processed Potatoes. Brazilian Archives of Biology and Technology. 54(5): 983-990. doi.org/10.1590/S1516-89132011000500016.
Walker, G.M. and Stewart, G. 2016. Saccharomyces cerevisiae in the Production of Fermented Beverages. Beverages. 2: 30. 10.3390/beverages2040030.
Wisal, S.,Ullah, J., Zeb A. and Khan, M.Z. 2013. Effect of Refrigeration Temperature, Sugar Concentrations and Different Chemicals Preservatives on the Storage Stability of Strawberry Juice. International Journal of Engineering and Technology. 13(2): 160-168.
Zaouay, F., Hadj Salem, H., Labidi, R. And Mars, M. 2014. Development and quality assessment of new drinks combining sweet and sour pomegranate juices. Emirates Journal of Food and Agriculture. 26 (1): 01-08. doi.org/10.9755/ejfa.v26i1.14838.
Zarfeshany, A., Asgary, S. and Javanmard, S.H. 2014. Potent health effects of pomegranate. Advanced Biomed Research. 3: 100. doi.10.4103/2277-9175.129371.
Zhang, Z., Pang, X., Xuewu, D., Jia, Z. and Jiang, Y. 2005. Role of peroxidase in anthocyanin degradation in litchi fruit pericarp. Food Chemistry. 90(1-2): 47-52. doi.org/10.1016/j.foodchem.2004.03.023.
DOI: https://doi.org/10.22146/ifnp.62938
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