Nutritional and Compositional Changes in α-tomatine Rich Ready-To-Serve Beverage From Matured Green Tomato (Solanium Lycopersium)

Vithal Balavant Kudachikar(1*), P Geethanjali(2), Prakash Om(3), Kumar Amarjeet(4), Athar Singh Chauhan(5)

(1) CSIR, New Delhi
(2) CSIR, New Delhi, India
(3) CSIR, New Delhi, India
(4) CSIR, New Delhi, India; Rohtas Mahila College, Sasaram; Veer Kunwar Singh University, Ara, India
(5) CSIR, New Delhi, India
(*) Corresponding Author


The study aimed to develop and evaluate the storage stability of α-Tomatine rich ready-to-serve (RTS) beverages from green tomato juice at low temperature (LT, 4±1 °C) up to 90 days. The results indicated the α-tomatine content in green tomato juice and in RTS beverage was found to be 43.23±3.59 mg/100 mL and 3.94±0.21 mg/100 mL respectively. The stability of the product stored in a Brown glass bottle (BB) container was excellent with better retention of α-Tomatine (2.31±0.29 mg/100 mL), total phenolic content (1.42 mg GAE/100 mL), ascorbic acid (17.66 mg/100 mL), total chlorophyll (1.07 mg/100mL) and viscosity (15.46 cp), with high sensory scores (7.6) as compared to the quality of the product (with 6.8 sensory scores)stored in a white glass bottle (WB) container. The microbial counts of products stored in both containers after 90 days indicated within the permissible limit.


α-tomatine; chlorophyll; green tomato juice; RTS Beverage; storage stability ; overall acceptibilty

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AOAC. 1990. Official Methods of Analysis.15th Edition. Association of Official Analytical Chemist, Virginia, USA.

AOAC. 1995. Official Methods of Analysis.16thedition.Association of Official Analytical Chemists, Washington DC, USA.

APHA. 1984. Technical Committee on Microbiological Methods for Foods. In “Compendium of Methods for the Microbiological Examination of Foods”. ed. M.L.Speck, 2nd ed. American Public Health Association, Washington DC,

Chen, B.H. and Chen, Y.Y. 1993. Stability of chlorophylls and carotenoids in sweet potato leaves during microwave cooking. Journal of Agricultural Food Chemistry. 41:1315-1320.

Da Costa, G.M., De Carvalho Silva, J.V., Mingotti, J.D., Barão, C.E., Klososki, S.J. and Pimentel, T. C. 2017. Effect of ascorbic acid or oligofructose supplementation on L. paracasei viability, physicochemical characteristics and acceptance of probiotic orange juice. LWT - Food Science and Technology. 75:195-201.

Dzyadevych, S.V., Arkhypova, V.N., Soldatkin, A.P., El'skaya, A.V., Martelet, C. and Jaffrezic- Renault, N. 2004. Enzyme biosensor for tomatine detection in tomatoes. Analytical Letters. 37:1611-1624.

Friedman, M. 2013. Anticarcinogenic, cardioprotective, and other health benefits of tomato compounds lycopene, α-tomatine, and tomatidine in pure form and in fresh and processed tomatoes. Journal of Agricultural Food Chemistry. 61 : 9534-9550.

Friedman, M., Henika, P.R. and Mackey, B.E. 2003. Effect of feeding solanidine, solasodine and tomatidine to non-pregnant and pregnant mice. Food Chemistry and Toxicology. 41: 61-71.

Friedman, M., Levin, C.E., Lee, S.U., Kim, H.J., Lee, I.S., Byun, J.O. and Kozukue, N. 2009. Tomatine-containing green tomato extracts inhibit growth of human breast, colon, liver, and stomach cancer cells. Journal of Agricultural Food Chemistry. 57: 5727-5733.

FSSAI. 2017. Directions Under Section 16 (5) of the Food Safety and Standards Act, 2006, FDA Bhawan, Kotla Road, New Delhi.India.

Gulcin, Y., Elias, R., Gepdiremen, A., Boyer, L. and Koksal, E. 2007. A comparative study on the antioxidant activity of fringe tree (Chionanthus virginicus L.) extracts. African Journal of Biotechnology. 6 : 410-418.

Hemalatha, R., Kumar, A., Prakash, O., Supriya, A., Chauhan, A.S. and Kudachikar, V. B. 2018. Development and quality evaluation of ready to serve (RTS) beverage from Cape Gooseberry (Physalis peruviana L.). Beverages. 4 : 42.

Itkin, M., Rogachev, I., Alkan, N., Rosenberg, T., Malitsky, S., Masini, L. and Prusky, D. 2011. Glycoalkaloid Metabolism1 is required for steroidal alkaloid glycosylation and prevention of phytotoxicity in tomato. The Plant Cell. 23: 4507-4525.

Jadhav, S.B., Joshi, G.D., and Garande, V.K. 2006. Studies on preparation and storage of raw and ripe kokum fruit products. Journal of Asian Horticulture. 2 : 205-207.

Jakhar, M.S. and Pathak, S. 2012. Studies on the preparation and storage stability of blended ready-to-serve from ber (Zizyphus mauritiana lamk.) and Jamun (Syzygium cumini Skeels.) pulp. Plant Archives. 12: 533-536.

Kaul, R.K., Gupta, N., Sharma, I. and Gupta, V. 2009. Dietary fibres: a review. Beverage Food World. 36: 23-27.

Kozukue, N. and Friedman, M. 2003. Tomatine, chlorophyll, β‐carotene and lycopene content in tomatoes during growth and maturation. Journal of Science of Food and Agriculture. 83:195-200.

Lee, K.R., Kozukue, N., Han, J.S., Park, J.H., Chang, E.Y., Baek, E.J. and Friedman, M. 2004. Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells. Journal of Agriculture and Food Chemistry. 52: 2832-2839.

Lee, S.T., Wong, P.F., Cheah, S.C. and Mustafa, M.R. 2011. Alpha-tomatine induces apoptosis and inhibits nuclear factor-kappa B activation on human prostatic adenocarcinoma PC-3 cells. PLoS One. 6 : e18915.

Meher, H.C. and Gaur, H.S. 2003. A new UV-LC method for estimation of a-tomatine and a-solanine in tomato. Indian Journal of Nematology. 33: 24-28.

Mishra, L.K. and Sangma, D. 2017. Quality attributes, phytochemical profile and storage stability studies of functional ready to serve (RTS) drink made from blend of Aloe vera, sweet lime, amla and ginger. Journal of Food Science and Technology. 54: 761-769.

Nidhi, G.R., Singh, R. and Rana, M.K. 2008. Changes in chemical composition of ready-to-serve bael-guava blended beverage during storage. Journal of Food Science and Technology (Mysore). 45:378-380.

Nisar, R., Baba, W.N. and Masoodi, F. A. 2015. Effect of chemical and thermal treatments on quality parameters and antioxidant activity of apple (pulp) grown in high Himalayan regions. Cogent Food and Agriculture. 1: 1063797.

Pawar, V.N., Kardile, W.G. and Hashmi, S.I. 2011. Effect of heat processing on discoloration of custard apple (Annona squamosa L.) fruit pulp and changes in quality characteristics during storage. Electronic Journal of Environmental Agriculture and Food Chemistry. 10 : 2098-2113.

Porto, M.R.A., Okina, V.S., Pimentel, T.C. and Prudencio, S.H. 2017. Physicochemical stability, antioxidant activity, and acceptance of beet and orange mixed juice during refrigerated storage. Beverages. 3:36.

Ranganna, S. 1999. “Handbook of Analysis of Fruit and Vegetable Products”. Tata McGraw-Hill Companies, New Delhi.

Sharma, M., Gehlot, R., Singh, R. and Siddiqui, S. 2012. Changes in chemical constituents and overall acceptability of guava jamun blend RTS drink and squash during storage. Beverage Food World. 39 : 39-42.

Sindumathi, G. and Premalatha, M. R. 2013. Development and storage studies of naturally flavored papaya-pineapple blended ready-to-serve (RTS) beverages. International Journal of Scientific Research. 4:856-860.

Singleton, V.L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16 : 144-158.

Tajner-Czopek, A., Rytel, E., Kita, A., Pęksa, A. and Hamouz, K. 2012. The influence of thermal process of coloured potatoes on the content of glycoalkaloids in the potato products. Food Chemistry. 133 : 1117-1122.

Waskar, D. P. and Garande, V. K. 2003. Studies on blending of pomegranate and kokum juices. Beverages Food World. 30: 27-28.

Wold, J. P., Veberg, A., Nilsen, A., Iani, V., Juzenas, P. and Moan, J. 2005. The role of naturally occurring chlorophyll and porphyrins in light-induced oxidation of dairy products. A study based on fluorescence spectroscopy and sensory analysis. International Dairy Journal. 15:343-353. doi:10.1016/j.idairyj.2004.08.009

Yadav, R. B., Yadav, B.S. and Kalia, N. 2010. Banana Herbal (Mentha arvensis) Beverage. American Journal of Food Technology. 5 : 121-129.


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