Optimization of Ultrasound-Assisted Cold Brew Process to Develop Phenolics, Flavonoids, and Caffeine-Rich Coffee Beverage


Andika Wicaksono Putro(1*), Fahmi Maulana Zulkarnaen(2), Aryudiana Sari(3), Sri Anggrahini(4), Widiastuti Setyaningsih(5)

(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(4) Universitas Gadjah Mada
(5) Universitas Gadjah Mada
(*) Corresponding Author


Ultrasound-assisted cold brew (UACB) method emerged as a solution of a long brew traditional cold brew method in coffee. Apart from the particular taste and odor, cold brew coffee contains a number of phenolic (TPC), flavonoid compounds (TFC), and caffeine. The level of these compounds in the drinks is strongly influenced by various factors related to extraction process, such as extraction temperature (x1: 4 and 25 °C), time (x2: 5,15, and 25 min), UAE duty cycle (x3: 20, 50, and 80 s-1), and grind size (x4: coarse and medium). Based on the TPC, TFC, and caffeine contents, the optimum condition was at 25 °C for 25 min with 80 s-1 UAE duty cycle and medium coffee grind size: with 2.40 ± 0.11 mg GAE/mL, 1.69 ± 0.05 mg RE/mL, and 1.10 ± 0.02 mg caffeine/mL, respectively. Compared with the traditional cold brew method, only the TPC and caffeine content were significantly lower than the UACB method (p < 0.05). Furthermore, the TPC and TFC were stable in 7-day refrigerator storage (p > 0.05). The IC50 values of UACB coffee were 7,487 mg/L for DPPH assay and 64,113 mg/L for ABTS assay.



cold brew coffee; ultrasound-assisted extraction; phenolic; caffeine; antioxidant activity

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Abid, M., Jabbar, S., Wu, T., Hashim, M.M., Hu, B., Lei, S., Zhang, X., Zeng, X., 2013. Effect of ultrasound on different quality parameters of apple juice. Ultrason. Sonochem. 20, 1182–1187. https://doi.org/10.1016/j.ultsonch.2013.02.010

Ahmed, M., Jiang, G.H., Park, J.S., Lee, K.C., Seok, Y.Y., Eun, J.B., 2019. Effects of ultrasonication, agitation and stirring extraction techniques on the physicochemical properties, health-promoting phytochemicals and structure of cold-brewed coffee. J. Sci. Food Agric. 99, 290–301. https://doi.org/10.1002/jsfa.9186

Bhat, R., Goh, K.M., 2017. Sonication treatment convalesce the overall quality of hand-pressed strawberry juice. Food Chem. 215, 470–476. https://doi.org/10.1016/j.foodchem.2016.07.160

Cämmerer, B., Kroh, L.W., 2006. Antioxidant activity of coffee brews. Eur. Food Res. Technol. 223, 469–474. https://doi.org/10.1007/s00217-005-0226-4

Carrera, C., Ruiz-Rodríguez, A., Palma, M., Barroso, C.G., 2012. Ultrasound assisted extraction of phenolic compounds from grapes. Anal. Chim. Acta 732, 100–104. https://doi.org/10.1016/j.aca.2011.11.032

Castañeda-Rodríguez, R., Mulík, S., Ozuna, C., 2020. Brewing Temperature and Particle Size Affect Extraction Kinetics of Cold Brew Coffee in Terms of Its Physicochemical, Bioactive, and Antioxidant Properties. J. Culin. Sci. Technol. 00, 1–22. https://doi.org/10.1080/15428052.2020.1848683

Cesaro, A., Russo, E., Crescenzi, V., 1976. Thermodynamics of caffeine aqueous solutions. J. Phys. Chem. 80, 335–339.

Ceylan, E., 2019. Food Safety Considerations for Coffee and Tea Brewers [WWW Document]. Merieux Nutr. URL http://foodsafety.merieuxnutrisciences.com/2019/05/23/food-safety-considerations-for-coffee-and-tea-brewers/ (accessed 5.6.21).

Chemat, F., Rombaut, N., Sicaire, A.G., Meullemiestre, A., Fabiano-Tixier, A.S., Abert-Vian, M., 2017. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason. Sonochem. 34, 540–560. https://doi.org/10.1016/j.ultsonch.2016.06.035

Claassen, L., Rinderknecht, M., Porth, T., Röhnisch, J., Seren, H.Y., Scharinger, A., Gottstein, V., Noack, D., Schwarz, S., Winkler, G., Lachenmeier, D.W., 2021. Cold brew coffee—pilot studies on definition, extraction, consumer preference, chemical characterization and microbiological hazards. Foods 10. https://doi.org/10.3390/foods10040865

Clarke, R.J., 1985. Coffee Volume 1: Chemistry, 1st ed. Springer Netherlands, Netherlands. https://doi.org/10.1007/978-94-009-4948-5

Coelho, C., Ribeiro, M., Cruz, A.C.S., Domingues, M.R.M., Coimbra, M.A., Bunzel, M., Nunes, F.M., 2014. Nature of phenolic compounds in coffee melanoidins. J. Agric. Food Chem. 62, 7843–7853. https://doi.org/10.1021/jf501510d

Cordoba, N., Pataquiva, L., Osorio, C., Moreno, F.L.M., Ruiz, R.Y., 2019. Effect of grinding, extraction time and type of coffee on the physicochemical and flavour characteristics of cold brew coffee. Sci. Rep. 9, 1–12. https://doi.org/10.1038/s41598-019-44886-w

Farah, A., Donangelo, C.M., 2006. Phenolic compounds in coffee. Brazilian J. Plant Physiol. 18, 23–36. https://doi.org/10.1590/S1677-04202006000100003

Fuller, M., Rao, N.Z., 2017. The Effect of Time , Roasting Temperature , and Grind Size on Caffeine and Chlorogenic Acid Concentrations in Cold Brew Coffee. Sci. Rep. 1–9. https://doi.org/10.1038/s41598-017-18247-4

Górecki, M., Hallmann, E., 2020. The antioxidant content of coffee and its in vitro activity as an effect of its production method and roasting and brewing time. Antioxidants 9. https://doi.org/10.3390/antiox9040308

Gumulya, D., Helmi, I.S., 2017. Kajian Budaya Minum Kopi Indonesia. J. Dimens. Seni Rupa dan Desain 13, 153. https://doi.org/10.25105/dim.v13i2.1785

Hainil, S., Suhaera, S., Lirtri, L., 2019. Quantitative Analysis of Caffeine Levels in Local Coffee (Coffea sp) Powder on Dabo Island with UV-Vis Spectrophotometry. Borneo J. Pharm. 2, 82–86. https://doi.org/10.33084/bjop.v2i2.897

Jung, S., Kim, M.H., Park, J.H., Jeong, Y., Ko, K.S., 2017. Cellular Antioxidant and Anti-Inflammatory Effects of Coffee Extracts with Different Roasting Levels. J. Med. Food 20, 626–635. https://doi.org/10.1089/jmf.2017.3935

Kedare, S.B., Singh, R.P., 2011. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 48, 412–422. https://doi.org/10.1007/s13197-011-0251-1

Kumar, S., Sandhir, R., Ojha, S., 2014. Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Res. Notes 7, 1–9. https://doi.org/10.1186/1756-0500-7-560

Kwok, R., Lee Wee Ting, K., Schwarz, S., Claassen, L., Lachenmeier, D.W., 2020. Current Challenges of Cold Brew Coffee—Roasting, Extraction, Flavor Profile, Contamination, and Food Safety. Challenges 11, 26. https://doi.org/10.3390/challe11020026

Labbé, D., Têtu, B., Trudel, D., Bazinet, L., 2008. Catechin stability of EGC- and EGCG-enriched tea drinks produced by a two-step extraction procedure. Food Chem. 111, 139–143. https://doi.org/10.1016/j.foodchem.2008.03.048

Matuszewska, A., Jaszek, M., Stefaniuk, D., Ciszewski, T., Matuszewski, Ł., 2018. Anticancer, antioxidant, and antibacterial activities of low molecular weight bioactive subfractions isolated from cultures of wood degrading fungus Cerrena unicolor. PLoS One 13, e0197044. https://doi.org/10.1371/journal.pone.0197044

Mestdagh, F., Glabasnia, A., Giuliano, P., 2017. The Brew-Extracting for Excellence. Cr. Sci. Coffee 355–380. https://doi.org/10.1016/B978-0-12-803520-7.00015-3

Nijveldt, R.J., Van Nood, E., Van Hoorn, D.E.C., Boelens, P.G., Van Norren, K., Van Leeuwen, P.A.M., 2001. Flavonoids: A review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 74, 418–425. https://doi.org/10.1093/ajcn/74.4.418

Parliment, T.H., 2000. An overview of coffee roasting. ACS Symp. Ser. 754, 188–201. https://doi.org/10.1021/bk-2000-0754.ch020

Pinto, P.C.A.G., Saraiva, M.L.M.F.S., Reis, S., Lima, J.L.F.C., 2005. Automatic sequential determination of the hydrogen peroxide scavenging activity and evaluation of the antioxidant potential by the 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay in wines by sequential injection analysis. Anal. Chim. Acta 531, 25–32.

Portela, C. da S., Almeida, I.F. de, Mori, A.L.B., Yamashita, F., Benassi, M. de T., 2021. Brewing conditions impact on the composition and characteristics of cold brew Arabica and Robusta coffee beverages. Lwt 143. https://doi.org/10.1016/j.lwt.2021.111090

Rao, N.Z., Fuller, M., 2018. Acidity and Antioxidant Activity of Cold Brew Coffee. Sci. Rep. 1–9. https://doi.org/10.1038/s41598-018-34392-w

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231–1237.

Rodríguez-Artalejo, F., López-García, E., 2018. Coffee Consumption and Cardiovascular Disease: A Condensed Review of Epidemiological Evidence and Mechanisms. J. Agric. Food Chem. 66, 5257–5263. https://doi.org/10.1021/acs.jafc.7b04506

Sánchez-Moreno, C., Larrauri, J.A., Saura-Calixto, F., 1998. A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric. 76, 270–276. https://doi.org/https://doi.org/10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9

Sopelana, P., Pérez-Martínez, M., López-Galilea, I., de Peña, M.P., Cid, C., 2013. Effect of ultra high temperature (UHT) treatment on coffee brew stability. Food Res. Int. 50, 682–690. https://doi.org/10.1016/j.foodres.2011.07.038

Taubert, D., Breitenbach, T., Lazar, A., Censarek, P., Harlfinger, S., Berkels, R., Klaus, W., Roesen, R., 2003. Reaction rate constants of superoxide scavenging by plant antioxidants. Free Radic. Biol. Med. 35, 1599–1607. https://doi.org/10.1016/j.freeradbiomed.2003.09.005

Tazzeo, T., Bates, G., Roman, H.N., Lauzon, A.M., Khasnis, M.D., Eto, M., Janssen, L.J., 2012. Caffeine relaxes smooth muscle through actin depolymerization. Am. J. Physiol. - Lung Cell. Mol. Physiol. 303, 334–343. https://doi.org/10.1152/ajplung.00103.2012

Van Der Sluis, A.A., Dekker, M., Van Boekel, M.A.J.S., 2005. Activity and concentration of polyphenolic antioxidants in apple juice. 3. Stability during storage. J. Agric. Food Chem. 53, 1073–1080. https://doi.org/10.1021/jf040270r

Yuliantari, N.W.A., Widarta, I.W.R., Permana, I.D.G.M., 2017. Pengaruh suhu dan waktu ekstraksi terhadap kandungan flavonoid dan aktivitas antioksidan daun sirsak (Annona muricata L.) menggunakan ultrasonik. Media Ilm. Teknol. Pangan (Scientific J. Food Technol. 4, 35–42.

Zin, Z.M., Abdul Hamid, A., Osman, A., Saari, N., 2006. Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L.). Food Chem. 94, 169–178. https://doi.org/10.1016/j.foodchem.2004.08.048

DOI: https://doi.org/10.22146/ifnp.65741

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