Antioxidant Capacity of Black Tea Obtained Using Tyrosinase and Tannase Treatment

https://doi.org/10.22146/ifnp.64516

Fiyan Maulana Rajendra(1), Rachmad Gunadi(2), Supriyadi Supriyadi(3*)

(1) Faculty of Agricultural Technology, Universitas Gadjah Mada
(2) Faculty of Agriculture, Universitas Gadjah Mada
(3) Faculty of Agricultural Technology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Black tea has a lower antioxidant capacity than other teas, such as green tea, white tea, and oolong tea. Tannase and tyrosinase can be used as a treatment to improve the quality of black tea infusion. Tannase has been reported to be an effective way to enhance antioxidant activity in black tea infusion. Meanwhile, tyrosinase could produce higher theaflavin content than thearubigin. Research about Ready-to-Drink (RTD) black tea preparation with the addition of tannase and tyrosinase to fresh tea leaves before pasteurization has not been reported. This study aimed to find a good combination of tannase (1 mg/ml) and tyrosinase (111; 446; 1785 U/ml) to produce high antioxidant activity of RTD black tea. The results showed that higher tyrosinase concentration decreased the antioxidant activity (DPPH and reducing power), epicatechin (EC), epigallocatechin (EGC), and epigallocatechin gallate (EGCG) content yet increased the theaflavin content and theaflavin (TF)/thearubigin (TR) ratio in the tannase-tyrosinase treated black tea. Still, the highest concentration of tyrosinase (1785 U/ml) in tannase-tyrosinase black tea produces higher antioxidant activities, gallic acid, EC, and EGC content than commercial black tea and tyrosinase without tannase-treated black tea. Thus, the combination of tannase (1 mg/ml) and tyrosinase (1785 U/ml) could be the best treatment to produce high-antioxidant black tea. 


Keywords


Tannase; Tyrosinase; Black Tea; Antioxidant

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References

Bae, Y. W., Lee, S. M., and Kim, K. O. (2016). Age and gender differences in the influence of extrinsic product information on acceptability for RTD green tea beverages. Journal of the Science of Food and Agriculture, 96 (4), 1362–1372.

Beniwal, V., Kumar, A., Sharma, J., and Chhokar, V. (2013). Recent advances in industrial application of tannases: A review. Recent Patents on Biotechnology, 7 (3), 228–233.

Benzi, I.F.F., and Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239 (292), 70–76.

Borse, B.., and Rao, L. J.. (2012). Novel Bio-Chemical Profiling of Indian Black Teas with Reference to Quality Parameters. Journal of Microbial & Biochemical Technology S14, (14), 1–16.

Cao, Q. Q., Zou, C., Zhang, Y. H., Du, Q. Z., Yin, J.F., Shi, J., Xue, S., and Xu, Y.Q. (2019). Improving the taste of autumn green tea with tannase. Food Chemistry, 277, 432–437.

Carloni, P., Tiano, L., Padella, L., Bacchetti, T., Customu, C., Kay, A., and Damiani, E. (2013). Antioxidant activity of white, green and black tea obtained from the same tea cultivar. Food Research International, 53 (2), 900–908.

Chandini, S. K., Rao, L. J., Gowthaman, M. K., Haware, D.J., and Subramanian, R. (2011). Enzymatic treatment to improve the quality of black tea extracts. Food Chemistry, 127 (3), 1039–1045.

Chang, K. (2015). World tea production and trade, current and future development. Food and Agriculture Organization of the United Nations: Rome.

Chaturvedula, V. S. P., and Prakash, I. (2011). The aroma, taste, color and bioactive constituents of tea. Journal of Medicinal Plants Research, 5 (11), 2110–2124.

Chen, Y., Huang, J.-Y., Lin, Y., Lin, I.-F., Lu, Y.-R., Liu, L.-H., and Wang, H.-M.D. (2018). Antioxidative and Antimelanoma Effects of Various Tea Extracts via a Green Extraction Method. Journal of Food Quality, 2018, 1–6.

Dubey, K. K., Janve, M., Ray, A., and Singhal, R. S. (2020). Ready to Drink Tea In Trends in Non-alcoholic Beverages, pp. 101–140. Woodhead Publishing: Cambridge.

Engelhardt, U. H. (2010) Chemistry of Tea In Comprehensive Natural Products II: Chemistry and Biology, pp. 999–1032. Elsevier Ltd.

Fadlillah, H. N., Ramadhan, H.S., Hermanianto, J., and Felanesa, L. (2020). Study on sweetener selection in RTD tea beverages. Jurnal Teknologi dan Industri Pangan, 31 (1), 1–8.

Halder, B., Pramanick, S., Mukhopadhyay, S., and Giri, A. K. (2006). Anticlastogenic effects of black tea polyphenols theaflavins and thearubigins in human lymphocytes in vitro. Toxicology in Vitro, 20 (5), 608–613.

Hong, Y. H., Jung, E. Y., Shin, K. S., Yu, K. W., Chang, U.J., and Suh, H.J. (2013). Tannase-converted green tea catechins and their anti-wrinkle activity in humans. Journal of Cosmetic Dermatology, 12 (2): 137–143.

Huang, S.-T., Hung, Y.-A., Yang, M.-J., Chen, I.-Z., Yuann, J.-M.P., and Liang, J.-Y. (2019). Effects of Epigallocatechin Gallate on the Stability of Epicatechin in a Photolytic Process. Molecules, 24 (787), 1–13.

Imran, A., Arshad, M. U., Arshad, M. S., Imran, M., Saeed, F., and Sohaib, M. (2018). Lipid peroxidation diminishing perspective of isolated theaflavins and thearubigins from black tea in arginine induced renal malfunctional rats. Lipids in Health and Disease, 17 (1), 1–12.

Kumar, C.S., Subramanian, R., and Rao, L. J. (2013). Application of Enzymes in the Production of RTD Black Tea Beverages: A Review. Critical Reviews in Food Science and Nutrition, 53 (2), 180–197.

Li, J., Xiao, Q., Huang, Y., Ni, H., Wu, C., and Xiao, A. 2017. Tannase application in secondary enzymatic processing of inferior Tieguanyin oolong tea. Electronic Journal of Biotechnology, 28, 87–94.

Lin, S. D., Udompornmongkol, P., Yang, J. H., Chen, S. Y., and Mau, J. L. (2014). Quality and antioxidant property of three types of tea infusions. Journal of Food Processing and Preservation, 38 (4), 1401–1408.

Lu, M. J., Chu, S. C., Yan, L., and Chen, C. (2009). Effect of tannase treatment on protein-tannin aggregation and sensory attributes of green tea infusion. LWT - Food Science and Technology, 42 (1), 338–342.

Majchrzak, D., Mitter, S., and Elmadfa, I. (2004). The effect of ascorbic acid on total antioxidant activity of black and green teas. Food Chemistry, 88, 447–451.

Murugesan, P., Venkateswaran, G., and Sathish, G. (2020). Enhancing the quality of naturally oxidized tea with ascorbic acid. International Journal of Advanced Engineering Research and Science, 7 (4), 403–409.

Nanjo, F., Mori, M., Goto, K., and Hara, Y. (1999). Radical scavenging activity of tea catechins and their related compounds. Bioscience, Biotechnology and Biochemistry, 63 (9), 1621–1623.

Narai-Kanayama, A., Kawashima, A., Uchida, Y., Kawamura, M., and Nakayama, T. (2017). Specificity of tyrosinase-catalyzed synthesis of theaflavins. Journal of Molecular Catalysis B: Enzymatic, 133, S452–S458.

Narai-Kanayama, A., Uchida, Y., Kawashima, A., and Nakayama, T. (2019). Elimination of hydrogen peroxide enhances tyrosinase-catalyzed synthesis of theaflavins. Process Biochemistry, 85, 19–28.

Owuor, P. O., and Reeves, S. G. (1986). Optimising fermentation time in black tea manufacture. Food Chemistry, 21 (3), 195–203.

Raghuwanshi, S., Misra, S., and Saxena, R.K. (2012). Enzymatic treatment of black tea (ctc and kangra orthodox) using Penicillium charlesii tannase to improve the quality of tea. Journal of Food Processing and Preservation, 37 (5), 855–863.

Sanderson, G.W., and Coggon, P. (1974). Green Tea Conversion Using Tannase and Natural Tea Enzymes. United States Patent ( 19 ) (19): .

Skotnicka, M., Chorostowska-Wynimko, J., Jankun, J., and Skrzypczak-Jankun, E. (2011). The black tea bioactivity: An overview. Central-European Journal of Immunology, 36 (4), 284–292.

Takeuchi, Y., Okuno, K., Yoshioka, H., and Yoshioka, H. (2007). Characteristics of the OH radical scavenging activity of tea catechins. Journal of Radioanalytical and Nuclear Chemistry, 272 (3), 455–459.

Thea, A. E., Lloret, M. A., Brumovsky, L. A., and Schmalko, M.E. (2012). Differences in quality parameters between types of commercial tea from Argentina. International Journal of Food Studies, 1 (2), 168–178.

Verloop, A. J. W., Gruppen, H., Bisschop, R., and Vincken, J. P. (2016). Altering the phenolics profile of a green tea leaves extract using exogenous oxidases. Food Chemistry, 196, 1197–1206.

Verloop, A.J.W., Vincken, J.P., and Gruppen, H. (2016). Peroxidase can perform the hydroxylation step in the ‘oxidative cascade’ during oxidation of tea catechins. Journal of Agricultural and Food Chemistry 64, (42), 8002–8009.

Wu, C.D., and Wei, G.-X. (2002). Tea as a Functional Food for Oral Health. Nutrition and Oral Health, 18 (05), 443–444.

Xu, X. Y., Meng, J. M., Mao, Q. Q., Shang, A., Li, B. Y., Zhao, C. N., Tang, G. Y., Cao, S. Y., Wei, X. L., Gan, R.Y., Corke, H., and Li, H. Bin. (2019). Effects of tannase and ultrasound treatment on the bioactive compounds and antioxidant activity of green tea extract. Antioxidants, 8 (362), 1–14.

Yabuki, C., Yagi, K., and Nanjo, F. (2017). Highly efficient synthesis of theaflavins by tyrosinase from mushroom and its application to theaflavin related compounds. Process Biochemistry, 55, 61–69.

Yoshino, K., Hara, Y., Sano, M., and Tomita, I. (1994). Antioxidative effects of black tea theaflavins and thearubigin on lipid peroxidation on rat liver homogenates induces by tert-butyl hydroperoxide. Biological and Pharmaceutical Bulletin, 17 (1), 146–149.

Zaiter, A., Becker, L., Karam, M.C., and Dicko, A. (2016). Effect of particle size on antioxidant activity and catechin content of green tea powders. Journal of Food Science and Technology, 53 (4), 2025–2032.

Zhang, X., and Du, X.-F. (2015). Effects of exogenous enzymatic treatment during processing on the sensory quality of summer tieguanyin oolong tea from the Chinese Anxi County. Food Technology and Biotechnology, 53 (2), 180–189.

Zhang, Y.N., Yin, J.F., Chen, J.X., Wang, F., Du, Q.Z., Jiang, Y.W., and Xu, Y.Q. (2016). Improving the sweet aftertaste of green tea infusion with tannase. Food Chemistry, 192, 470–476.

Zhu, K., Ouyang, J., Huang, J., and Liu, Z. (2020). Research progress of black tea thearubigins: a review. Critical Reviews in Food Science and Nutrition, 0 (0), 1–11.

Zolghadri, S., Bahrami, A., Tareq, M., Khan, H., and Saboury, A.A. (2019). A comprehensive review on tyrosinase inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry, 34 (1), 279–309.



DOI: https://doi.org/10.22146/ifnp.64516

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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