Chromatographic Profiles of Umami Fractions from Indonesian Commercial Salty Soy Sauce

Hanifah Nuryani Lioe(1*), Diana Ayu Nindita(2), Warsono El Kiyat(3)

(1) Faculty of Agricultural Engineering and Technology; Bogor Agricultural University.
(2) Sekolah Pascasarjana, S2 Ilmu Pangan, IPB Bogor
(3) Sekolah Pascasarjana, S2 Ilmu Pangan, IPB, Bogor
(*) Corresponding Author


Salty soy sauce subjected in this study is a variety of commercial soy products in Indonesia. Chromatographic profiles linked to taste dilution analysis of the soy sauce were analyzed by Sephadex G-15 gel filtration chromatography followed by RP-HPLC. The results showed that there were 4 umami fractions (Fractions I − IV) obtained by Sephadex G-15 separation. Chromatographic profiles at 254 nm could show the differentiation of the four fractions and then their RP-HPLC profiles were proven to be different from each other. Fraction III which contained 65% of the soy sauce dry matters, had the highest umami intensity with umami TD factor of 256, meanwhile, this fraction was tasted salty due to the salt contained in the soy sauce. Fraction III was dominated by the later peaks in the RP-HPLC chromatogram, which was more hydrophobic. The hydrophobic components were commonly tasted bitter, perhaps in the commercial salty soy sauce, the taste interaction between the umami and bitter components might have occurred.


nonvolatile components; RP-HPLC profile; salty soy sauce; taste dilution analysis, hydrophobic components

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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