The Effect Of Autoclaved – Cooled Jack Bean (Canavalia Ensiformis (L.) Dc.) High RS-4 Starch On Lowering Glucose Level And Characteristics Of Digesta Of Stz-Na Induced Type-2 Diabetes Mellitus Rats

https://doi.org/10.22146/ifnp.28539

Azkia Nastiti(1*), Agnes Murdiati(2), Y. Marsono(3)

(1) Department of Food and Agricultural Product Technology Faculty of Agricultural Technology Universitas Gadjah Mada
(2) Department of Food and Agricultural Product Technology Faculty of Agricultural Technology Universitas Gadjah Mada
(3) Department of Food and Agricultural Product Technology Faculty of Agricultural Technology Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Jack bean is one of local beans known to contain natural resistant starch (RS) content. To increase its RS content, four cycle autoclaving – cooling treatment modification was applied to generate resistant starch type 4 (RS-4) which refers to RS obtained from modification. RS is able to improve blood glucose level of diabetic individuals. This research aimed to measure the effect of jack bean autoclaved – cooled starch (AC starch) diet on blood glucose level and digesta characteristics of type-2 diabetic Sprague Dawley rats in vivo. Bioassay analyses showed that jack bean AC starch consumption decreased blood glucose level at range of 96.05mg/dl – 135.97mg/dl. The diet was also able to increase short chain fatty acids (SCFA) production in form of acetic acid, propionic acid, and butiric acid, to increase total weight and moisture content of digesta, while reduce digesta pH, as well as to inhibit glucose absorption. Diet of 50% high RS jack bean AC starch showed the best results in all glucose metabolism parameters for type-2 diabetic animals.

 


Keywords


jack bean starch; resistant starch (RS); RS 4; autoclaving – cooling; type-2 diabetes mellitus; blood glucose; short chain fatty acids (SCFA)

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DOI: https://doi.org/10.22146/ifnp.28539

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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