Determination of Antioxidant Activity and Phenolic Compounds of Methanolic Extract of Java Plum (Syzygium cumini Linn.) Seed

https://doi.org/10.22146/ifnp.24279

Rohadi Rohadi(1*), Umar Santoso(2), Sri Raharjo(3), Iip Izul Falah(4)

(1) Department of Processing Agricultural Product, Faculty of Agricultural Technology, Semarang University, Jl. Arteri Soekarno-Hatta, Semarang 50196, Indonesia
(2) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora No. 1, Bulaksumur , Yogyakarta 55281
(3) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Flora No. 1, Bulaksumur , Yogyakarta 55281, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Science, Gadjah Mada University, Jl. Sekip Utara, Yogyakarta 55281
(*) Corresponding Author

Abstract


Methanolic extract of Java Plum (Syzygium cumini L. (Skeel) seed (MEJS) is potential source of natural antioxidant. As indicated by several in vitro measurements, the extract had strong DPPH (1,1 diphenyl, 2–picryl hydrazyl) and ABTS (2,2-azinobis, 3-ethylbenzothiazoline-6-sulphonate) radical scavenging activity, strong Ferric Reducing Antioxidant Power (FRAP) and moderate inhibition activity of linoleic acid oxidation. This study aimed to determine antioxidant activity and phenolic compound of Java Plum seed (Syzygium cumini L. (Skeel) methanolic extract fractions. Phenolics compound identification using Thin Layer Chromatography (TLC) showed that all fractions (polar, semi polar and hydrolyzed semi polar fraction) contained Gallic acid, Tannic acid and flavonol’s Rutin. HPLC-DAD analysis showed that its polar fraction contained 25 ppm flavonol’s Quercetine and 55181 ppm flavonol’s (+)- Catechin, ethyl acetate fraction contained 54 ppm flavonol’s Rutin and 528 ppm (+)- Catechine, while hydrolyzed ethyl acetate fraction contained 404 ppm Rutin and 28692 ppm (+)- Catechine.

Keywords


Antioxidant, Catechine, Java Plum Seed, Methanolic Extract

Full Text:

PDF


References

Baliga, M. S., Baht, H. P., Baliga, B. R. V., Wilson, R. and Palatty, P. kayakL. 2011. Phytochemistry, traditional uses and pharmacology of Eugenia jambolana Lam. (black plum): A review.Food Research International. 44:1776-1789.

Brewer, M.S. 2011. Natural Antioxidant: Source, Compounds, Mechanisms of Action, and Potential Application. Comprehensive Reviews.Food Science and Food Safety. 10: 221-247.

Durackova, Z. 2008. Oxidants, Antioxidants, and Oxidative Stress. In “Mitochondrial Medicine: Mitochondrial Metabolism, Diseases, Diagnosis, and Therapy”. ed. nna Gvozdjakova, pp. 19-54.Springer-Verlag, New York.

Ebrahimzadeh, M.A., Pourmorad, F. and Hafezi, S. 2008. Antioxidant activities of Iranian Corn Silk. Turkish Journal of Biology.32: 43-49. ar orne, J.B. 2006. “Metode Fitokimia: Penuntun Cara Modern Menganalisis Tum uhan”. Institut Teknologi Bandung, Bandung.

Heim, K. E., Tagliaferro, A. R., and Bobilya, D. J. 2002. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. Review. Journal of Nutritional Biochemistry. 13:572–584.

Helmstadter, A. 2008. Syzygium cumini(L.) Skeel (Myrtaceae) against diabetes-125 years of research. Pharmazie. 63 (2):91-101.

Jayaprakasha, G.K., Singh, R.P. andSakariah, K.K. 2001. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73:285-290.

Kumar, A., Lakshman, K., Jayaveera, K. N., Tripathi, S. N. M. and Satish, K.V. 2010. Estimation of Gallic Acid, Rutin, and Quercetin in Terminalia chebulaby HPTLC. Jordan Journal of Pharmaceutical Sci. 3(1): 63-67.

Laguerre, M., Lecomte, J., andVilleneuve, P. 2007. Evaluation of the ability of antioxidant to counteract lipid oxidation: Existing methods, new trends, and challenges: A review. Progress in Lipid Research. 46:244-282.

Meena, M.C. and Patni, V. 2008. Isolation and Identification of Flavonoid “Quercetine” from Citrullus colocynthis (Linn.) Schrad. Asian J. Exp. Sci. 22 (1):137-142.

Mura, K., Shiramatsu, H. and Tanimura, W. 2000. A Substance Inhibiting the Growth of Lactic Acid Bacteria in Duhat (Syzygium cumini Skeels) Bark. Journal Biocontrol Science. 5(1):33-38.

Palici, I., Tita, B., Ursica, L., and Tita, D. 2005. Method for Quantitative Determination of Polyphenolic Compounds and Tannins from Vegetal Products. Seria F Chemia. 8:21-32

Phani, Ch. R. S., Vinaykumar, Ch., Rao, K. U. and Sindhuja, G. 2010. Quantitative Analysis of Quercetin in Natural Sources by RP-HPLC. International Journal of Research in Pharmaceutical and Biomedical Science. 1(1):19-22.

Parmar, J., Sharma, P. and Verma, P. 2010. Chemopreventive Action of Syzygium cuminion DMBA-Induced Skin Papillomagenesis in Mice. Asian Pacific Journal of Cancer Prevention. 11(1): 261-265.

Quisumbing, E. 1951. Medical Plant of the Philippines. Tech. Bui. Department of Agricultural and Natural Resources, Manila. In“Jamun (Syzygium cuminiL.): A Review of Its Food and Medicinal Uses”. ed. Swami, S.B., Thakor, N.S.J., Patil, M.M. andHaldankar, P.M. 2012. Food and Nutrition Science.3:1100-1117.

Rohadi, Raharjo, S., Falah, I. I., and Santoso, U. 2016. Antioxidant activity of duwet (Syzygium cuminiLinn.) seed extract Genthongvarieties on lipid peroxidation models in vitro. Journal of Agritech36 (1): 30-37.

Rydlewsky, A. A. de Morais, D.R., Rotta, E. M., Clause, T. and Visentainer, J. V. 2013. Determination of bioactive compounds, antioxidant activity, and physical and chemical composition of different parts of four Brazilian fruits. State University of Maringa, Postgraduate Program, Colombo, Brazil.

Saha, R. K., Zaman, N. M., and Roy, P. 2013. Comparative evaluation of the medicinal activities of methanolic extract of seed, fruit pulp and fresh juice of Syzygium cuminiin vitro. Journal of Coastal Medicine. 1(4):288-296.

Santagati, N. A., Salerno, L., Attaguile, G., Savoca Fr., and Ronsisvalle, G. 2008. Simultaneous Determination of Catechins, Rutin, and Gallic Acid in Cistus Species Extracts by HPLC with Diode Array Detection. Journal of Chromatographic Sci. 46:150-156.

Swami, SB., Thakor, NS., Patil, MM., and Haldankar, P M. 2012. Jamum (Syzygium cumini (L.)): A review of its food and medicinal uses. Food and Nutrition Sci. 3(8):1100-1117.

Tavares, I.M.C., Lago-Vanzela, E.S., Rebello, L.P.G., Ramos, A. M., Gomez-Alonzo, S., Garcia-Romero, E., Da-Silva, R., and Hermosin-Gutierrez, I. 2016. Comprehensive study of the phenolic composition of the edible part of Jambolan fruits (Syzygium cumini(L.) Skeel). Food Research International. 82:1-13.

Tupe, R.S., Kulkarni, A., Adeshara, K.M., Shaikh, S., Shah, N. andJadhav, A. 2015. Syzygium jambolana and Cephalandra indicahomeopathic preparation inhibit albumin glycation and protect erythrocytes: an in vitro study. Homeopathy. 104 (3): 197-204.

Vasi, S. and Austin, A. 2009. AntioxidantPotential of Eugenia jambolanaLam. Seeds. Journal of Biological Sciences. 9(8): 894-898.Zhang, L.L., andLin, Y.M. 2009. Antioxidant tannins from Syzygium cumin fruit. African Journal of Biotechnology. 8(10):2301-2309.

Zu, Y., Li, Ch., Fu, Y., Zhao, and Chunjian. 2006. Simultaneous determination of catechin, rutin, quercetin, kaempferol, and isorhamnetin in the extract of sea buckthorn (Hippopae rhamnoidesL.) leaves by RP-HPLC with DAD. Journal of Pharmaceutical and Biomedical Analysis.41: 714-719.



DOI: https://doi.org/10.22146/ifnp.24279

Article Metrics

Abstract views : 3533 | views : 3644

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

Journal of Indonesian Food and Nutrition Progress have been indexed by: 

   

 

This works is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.