Kinetics and Substrate Partitioning in the Polyphenol Oxidase-Catalysed Oxidation of Catechol in a Two-Phase System

Tyas Utami(1*), D.L Pyle(2)

(1) Gadjah Mada University
(2) University of Reading
(*) Corresponding Author


The kinetics of catechol oxidation catalysed by polyphenol oxidase in two-phase systems with mixtures of lauryl alcohol and hexane as the solvent phase is investigated, with particular reference to the effect of partitioning of catechol on the enzyme kinetics. Theory is developed to derive a relationship between the apparent K. and the intrinsic or 'true' Kn. The theory predicts that substrate partitioning should not change %Cue but that the relationship between the apparent and intrinsic K,, values should depend on the phase volume ratio and the partition coefficient. The theory shows good agreement with the results and gives a consistent K. value.

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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