Identification of Locus GH/Alui Polymorphisms of Kuantan and Pesisir Cattle

Hidayati Hidayati(1*), Robbana Saragih(2)

(1) Breeding and Reproduction Laboratory, Faculty of Agricultural and Animal Science, UIN Sultan Syarif Kasim Riau, Pekanbaru, 28292, Indonesia
(2) Breeding and Reproduction Laboratory, Faculty of Agricultural and Animal Science, UIN Sultan Syarif Kasim Riau, Pekanbaru, 28292, Indonesia
(*) Corresponding Author


Kuantan cattle is a local beef cattle of Riau Province that adaptive and breed well along the Kuantan river flow, found in Indragiri Hulu Regency and Kuantan Singingi Regency, have smaller morphometric performance compared to other local beef cattle and are relatively the same as Pesisir cattle. One of the genetic markers of growth determinant in beef cattle is the GH/AluI gene locus. Identification of the diversity of the GH/Alu I gene was carried out using the PCR-RFLP. Fifty four samples of Kuantan cattle consisting of 25 samples from Indragiri Hulu Regency and 29 samples from Kuantan Singingi Regency, as well as 25 samples of Pesisir cattle from BPTU-HPT Padang Mengatas Payakumbuh, West Sumatra, were used in this study. The oligonucleotida forward F-5’GCTGCTCCTGAGGGCCCTTC-3’ and reverse R-5’CATGACCCTCAGGTACGTCTCCG-3’ used in this study along 211 bp of GH Gene. The PCR mixture consisted of DNA templates 20 - 25 ng, 0.4 µL  primer forward dan primer reverse (10 ng primer forward and primer reverse), 25 µL dream tag green master mix and ddH2O up to a 50 µL. PCR machine conditions consisted of 940C pradenaturation for 5 minutes, 940C denaturation for 30 seconds, annealing 650C for 1 minute, 720C extension for 50 seconds and 720C final extension for 5 minutes. Identification of GH gene diversity using AluI at an incubation temperature of 370C for 3 hours. The results in this study were found monomorphic of GH/AluI genes in Kuantan and Pesisir cattle. The monomorphic in Kuantan cattle can be used as an indicator to assess the purity of Kuantan cattle in this areas.



AluI; GH Gene; Kuantan cattle; Monomorphic; Pesisir cattle

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Aruna, P., K., Chakravarty, T. K. Bhattacharya, B. K. Joshi and A. Sharma. 2004. Detection of polymorphisms of growth hormone gene for the analysis of relationship between allele type and growth traits in karan fries cattle. Asian-Aust. J. Anim. Sci. 17: 1334-1337. DOI:

Biswas, T. K., T. K. Bhattacharya, A. D. Narayan, S. Badola, P. Kumar and A. Sharma. 2003. Growth hormone gene polymorphisms and its effect on birth weight in cattle and buffalo. Asian-Aust J.Anim.Sci. 16: 494-497. DOI:

Boyer, R. 2005. Modern Experimental Biochemistry. Third Edition. Pearson Education, Inc. India

Curi, R. A., H. N. de Oliveira, A. C. Silveira and C. R. Lopes. 2005. Association between IGF-1, IGF-1R dan GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest. Sci. 94: 159-167.

Dario, C., D. Carnicella and G. Bufano. 2005. A note on the growth hormone (GH1-AluI) polymorphisms in podolian cattle in Southern Italy. Anim. Sci. Pap. Rep. 23: 43-49.

Dekkers, J. C. 2004. Comercial application of marker and gene assisted selection in livestock: strategis and lesson. J. Anim. Sci. 82 (E-Suppl): E313-E328.

Department of Animal Husbandry and Animal Health Riau Province. 2011. Laporan tahunan dinas peternakan dan kesehatan hewan provinsi Riau. Department of Animal Husbandry and Animal Health Riau Province, Pekanbaru.

Decree of The Minister of Agricultural Number: 2908/Kpts/OT.140/6/2011. Accessed 11 February 2020.

Decree of The Minister of Agricultural Number, 1052/Kpts/SR.120/10/2014. Accessed 10 Juni 2019.

Etherton, T. D. and D. E. Bauman. 1998. Biology of somatotropin in growth and lactation of domestic animals. Physol Rev. 78: 745-761.

Hediger, R., S. E. Johnson, W. Barendse, R. D. Drinkwater, S. S. Moore and J. Hetzel. 1990. Assigment of the growth hormone gene locus to 19q26-qtre in cattle and to 11q25-qter in sheep by in situ hybridization. Genomics. 8: 171 (Abstr).

Hidayati, R. Misrianti and A. Ali. 2016. Phylogenetic tree of Kuantan cattle by DNA Barcoding. JITV 21: 41-48.

Hidayati and T. Aulawi. 2016. Uji kualittaif dan kuantitatif hasil isolasi DNA berasal dari darah, feces dan urine pada ternak sapi, kerbau dan kambing. Laporan Penelitian. LPPM UIN SUSKA RIAU, Pekanbaru.

Jakaria, D. Duryadi, R. R. Noor, B. Tappa and H. Martojo. 2007. Evaluasi keragaman genetik gen hormon pertumbuhan (GH) pada sapi Pesisir Sumatera Barat menggunakan penciri PCR-RFLP. Media Peternakan 30: 1-10.

Jakaria, R. R. Noor, H. Martojo, D. Duryadi and B. Tappa. 2009. Identification of growth hormone (GH) gene MspI and AluI Loci polymorphism in beef cattles. The First International Seminar on Animal Industry. Faculty of Animal Science, Bogor Agricultural University, pp: 42-47.

Lawrence, T. L. J. and V. R. Fowler. 2002. Growth of Farm Animals. Walling Ford: CABI International. New York, USA.

Mu’in, M. A. 2008. Polimorfisme genetik Growth Hormone dan Insuline-Like Growth Factor-1 serta efeknya pada pertumbuhan prasapih sapi potong di Indonesia. Disertasi. Program Pascasarjana Peternakan, UGM, Yogyakarta.

Muin and Zurahmah. 2009. Evaluation of polymorphisms of Leu/Val of growth hormone gene in friesian holstein cows at Breeding Centre of Dairy Cattle Baturaden. Animal Production 11: 155-159.

Guo, S. W. and E. A. Thomson. 1992. Performing the exact test of Hardy- Weiberg proportion for multiple alleles. Biometric 48: 361-371.

Hartatik, T., D. Kurniawati and Adiarto. 2015. Assocoations between polymorphisms of growth hormone gene with milk production, fat and protein content in friesian holstein cattle. J. Indonesian Trop. Anim. Agric. 40: 133-137.

Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.

Nsubaga, A. M., M. M. Robbins, A. D. Roeder, P. A. Morin, C. Boesch and L. Vigilant. 2004. Factors Affecting The Amount of Genomic DNA Extracted From Ape Faeces and Identification of An Improved Sample Storage Method. Molecular Ecology 13: 2089-2094. doi:10.1111/j.1365-294x.2004.02207.x.

Qomar, W., M. R. Khan and A. Arafah. 2017. Optimazition of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method. Saudi Journal of Biological Sciences 24: 1465-1469.

Reis, C., D. Navas, M. Pereira and A. Cravador. 2001. Growth hormone AluI polymorphims analysis in eight portugase bovine breeds. Arch. Zootec. 50: 41-48.

Sambrook, J. and D. Russel. 2001. Molecular Clonning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, USA.

Sumantri, M. Imron, Sugyno, E. Andreas, R. Misrianti and A. B. L. Ishak. 2011. Keragaman grup gen hormon pertumbuhan (GH, GHR, GHRH dan Pit-1) dan hubungannya dengan superovulasi, tingkat ovulasi, tingkat fertilisasi dan kualitas embrio sapi di Balai Embrio Ternak (BET) Cipelang. JITV 16: 126-139.

Silveira, L. G. G., L. R. Furlan, R. A. Curi, A. L. J. Ferraz, M. M. de Beni, Arrigoni, L. Suguisawa, A. C. Silveira and H. N. de Oliveira. 2008. Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model. Genet. Mol. Biol. 31: 874-879.

Volkandari, S. D., T. Hartatik and Sumadi. 2013. Polimorfisme gen growth hormone pada sapi Limura. Buletin Peternakan 37: 67-73.

Widiarthi, N. P. F. O, K. Ratnayani and I. N. Wirajana. 2014. Pengaruh penmabahan susu skim terhadap hasil DNA Metagenomik diisolasi dari tanah hutan mangrove. Jurnal Kimia 8: 17-22.

Zulfikar. 2018. Performa kuantitatif sapi Kuantan dewasa berdasarkan warna bulu di Kecamatan Kelayang Kabupaten Indragiri Hulu. Skripsi, Fakultas Pertanian dan Peternakan UIN Suska Riau, Pekanbaru.


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