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Vol 15 No 1 (2021): Maret

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An Evaluation of the Antifungal and Antioxidant Activity of Pinus merkusii Bark Ethyl Acetate Extract

https://doi.org/10.22146/jik.v15i1.1494
Masendra Masendra
Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada
Brandon Aristo Verick Purba Purba
Department of Forest Products Technology, Faculty of Forestry, Universitas Gadjah Mada
Ganis Lukmandaru
glukmandaru@gmail.com
Faculty of Forestry, Universitas Gadjah Mada, Yogyakarta

Corresponding Author(s) : Ganis Lukmandaru

glukmandaru@gmail.com

Jurnal Ilmu Kehutanan, Vol 15 No 1 (2021): Maret

Abstract

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.

Keywords

P. merkusii bark extract antifungal steroids triterpenoids antioxidant
Masendra, M., Purba, B. A. V. P., & Lukmandaru, G. (2021). An Evaluation of the Antifungal and Antioxidant Activity of Pinus merkusii Bark Ethyl Acetate Extract . Jurnal Ilmu Kehutanan, 15(1), 102-110. https://doi.org/10.22146/jik.v15i1.1494
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