Antimicrobial Properties of Green Disinfectant from Citrus Waste-Infused Used Cooking Oil Using Conventional Method
Miradatul Najwa Muhd Rodhi(1*), Nur Affaaidil Amani Mohd Zaki(2), Harumi Veny(3), Fazlena Hamzah(4)
(1) School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia Biocatalysis and Biobased Materials Technology Research Group (BBMT), Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
(2) School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
(3) School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia Biocatalysis and Biobased Materials Technology Research Group (BBMT), Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
(4) School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia Biocatalysis and Biobased Materials Technology Research Group (BBMT), Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
(*) Corresponding Author
Abstract
This research aims to formulate a disinfectant from citrus waste-infused used cooking oil through the conventional process and evaluate its effectiveness in microbial elimination. Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS) were utilized to characterize citrus waste-infused used cooking oil. Two prominent bands belonging to the alkane (2921.93–2922.26 cm–1) and ester (1743.60–1743.73 cm–1) were observed on all FTIR spectra. Aside from that, through GC-MS analysis, dried orange-infused used cooking oil was discovered to have the highest percentage content of major antimicrobial compounds such as esters, oxygenated monoterpenoids, triterpenes, and alkaloids with 1.92% of the total amount of compounds found in the sample. However, the agar plate method revealed that the fresh lemon waste-infused used cooking oil disinfectant formulation was the most effective at inhibiting bacterial growth as the colony-forming detected on the agar plates dropped from 20 colonies to nearly zero and from 49 to 3 colonies for the plate swabbed with microbes from the table and doorknob surfaces, respectively. Based on the findings, the citrus waste and used cooking oil were viewed to have the potential as one of the possible ingredients in creating safer disinfectants in the future.
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[1] Pirofski, L., and Casadevall, A., 2012, Q & A: What is a pathogen? A question that begs the point, BMC Biol., 10 (1), 6.
[2] Drexler, M., Institute of Medicine, 2011, What You Need to Know About Infectious Disease, National Academies Press, Washington, DC.
[3] Suman, R., Javaid, M., Haleem, A., Vaishya, R., Bahl, S., and Nandan, D., 2020, Sustainability of coronavirus on different surfaces, J. Clin. Exp. Hepatol., 10 (4), 386–390.
[4] Azalee, N.I.W., El-Enshasy, H., Dailin, D.J., Manas, N.H.A., Malek, R.A., Mokhter, M.A., Salamun, N., and Rahman, R.A., 2020, Antimicrobial disinfectants and sanitizers: An effective tool for breaking the circle of pandemic disease, Asian J. Agric. Biol., 8 (3), 348–367.
[5] Luz, A., DeLeo, P., Pechacek, N., and Freemantle, M., 2020, Human health hazard assessment of quaternary ammonium compounds: Didecyl dimethyl ammonium chloride and alkyl (C12–C16) dimethyl benzyl ammonium chloride, Regul. Toxicol. Pharmacol., 116, 104717.
[6] Weatherly, L.M., and Gosse, J.A., 2017, Triclosan exposure, transformation, and human health effects, J. Toxicol. Environ. Health, Part B, 20 (8), 447–469.
[7] Satari, B., and Karimi, K., 2018, Citrus processing wastes: Environmental impacts, recent advances, and future perspectives in total valorization, Resour., Conserv. Recycl., 129, 153–167.
[8] Kheang, L.S., May, C.Y., Foon, C.S., and Ngan, M.A., 2006, Recovery and conversion of palm olein-derived used frying oil to methyl esters for biodiesel, J. Oil Palm Res., 18, 247–252.
[9] Mannu, A., Vlahopoulou, G., Urgeghe, P., Ferro, M., Del Caro, A., Taras, A., Garroni, S., Rourke, J.P., Cabizza, R., and Petretto, G.L., 2019, Variation of the chemical composition of waste cooking oils upon bentonite filtration, Resources, 8 (2), 108.
[10] Desbois, A.P., and Smith, V.J., 2010, Antibacterial free fatty acids: Activities, mechanisms of action and biotechnological potential, Appl. Microbiol. Biotechnol., 85 (6), 1629–1642.
[11] Sharma, K., Mahato, N., Cho, M.H., and Lee, Y.R., 2017, Converting citrus wastes into value-added products: Economic and environmentally friendly approaches, Nutrition, 34, 29–46.
[12] Madhuri, S., Ashwini, U.H., Srilakshmi, N.S., and Prashith Kekuda, T.R., 2014, Antimicrobial activity of Citrus sinensis and Citrus aurantium peel extracts, J. Pharm. Sci. Innovation, 3 (4), 366–368.
[13] Shareef, S.A., Hamasaeed, P.A., and Ismaeil, A.S., 2019, Sterilization of culture media for microorganisms using a microwave oven instead of autoclave, Rafidain J. Sci., 28 (1), 1–6.
[14] Nandiyanto, A.B.D., Oktiani, R., and Ragadhita, R., 2019, How to read and interpret FTIR spectroscopy of organic material, Indones. J. Sci. Technol., 4 (1), 97–118.
[15] Auta, M., Musa, U., Tsado, D.G., Faruq, A.A., Isah, A.G., Raji, S., and Nwanisobi, C., 2018, Optimization of citrus peels D-limonene extraction using solvent-free microwave green technology, Chem. Eng. Commun., 205 (6), 789–796.
[16] Mohamed, K.M., Al-Hazmi, A.H., Alasiri, A.M., and Ali, M.E.S., 2016, A GC-MS method for detection and quantification of cathine, cathinone, methcathinone and ephedrine in oral fluid, J. Chromatogr. Sci., 54 (8), 1271–1276.
[17] Coates, J., 2006, “Interpretation of Infrared Spectra, A Practical Approach” in Encyclopedia of Analytical Chemistry, Eds. Meyers, R.A., John Wiley & Sons, Inc., Hoboken, US, 1–23.
[18] Muhd Rodhi, M.N., Saifuddin, P.N.S., and Veny, H., 2020, Characterization of used cooking oil (UCO) and orange peels as the medium of insect repellent, Malay. J. Chem. Eng. Technol., 3 (2), 67–75.
[19] Abdul Wahab, A.A., Chang, S.H., and Md Som, A., 2015, Characterization of waste cooking oil as a potential green solvent for liquid-liquid extraction, International Conference on Advances in Civil and Environmental Engineering 2015, Universiti Teknologi Mara, Malaysia.
[20] Lim, S.F., Hamdan, A., Chua, D.S.N., and Lim, B.H., 2021, Comparison and optimization of conventional and ultrasound-assisted solvent extraction for synthetization of lemongrass (Cymbopogon)-infused cooking oil, Food Sci. Nutr., 9 (5), 2722–2732.
[21] Larayetan, R., Ololade, Z.S., Ogunmola, O.O., and Ladokun, A., 2019, Phytochemical constituents, antioxidant, cytotoxicity, antimicrobial, antitrypanosomal, and antimalarial potentials of the crude extracts of Callistemon citrinus, Evidence-Based Complementary Altern. Med., 2019, 5410923.
[22] El-Desoukey, R.M., Saleh, A.S., and Alhowamil, H.F., 2018, The phytochemical and antimicrobial effect of Citrus sinensis (Orange) peel powder extracts on some animal pathogens as eco-friendly, EC Microbiol., 14 (6), 312–318.
[23] Azmir, J., Zaidul, I.S.M., Rahman, M.M., Sharif, K.M., Mohamed, A., Sahena, F., Jahurul, M.H.A., Ghafoor, K., Norulaini, N.A.N., and Omar, A.K.M., 2013, Techniques for extraction of bioactive compounds from plant materials: A review, J. Food Eng., 117 (4), 426–436.
[24] Costa, S.S., Gariepy, Y., Rocha, S.C.S., and Raghavan, V., 2014, Microwave extraction of mint essential oil - Temperature calibration for the oven, J. Food Eng., 126, 1–6.
[25] Al–Âni, W., Tawfik, N., and Shehab, E., 2011, Antimicrobial activity of grapefruit seeds extracts (In vitro study), Al-Rafidain Dent. J., 11 (2), 341–345.
[26] Edogbanya, P.R.O., Suleiman, M.O., Olorunmola, J.B., and Oijagbe, I.J., 2019, Comparative study on the antimicrobial effects of essential oils from peels of three citrus fruits, MOJ Biol. Med., 4 (2), 49–54.
[27] Saleem, M., and Saeed, M.T., 2018, Potential application of waste fruit peels (orange, yellow lemon, and banana) as wide range natural antimicrobial agent, J. King Saud Univ., Sci., 32 (1), 805–810.
DOI: https://doi.org/10.22146/ijc.69812
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