Fast Analytical Method for Authentication of Chili Powder from Synthetic Dyes Using UV-Vis Spectroscopy in Combination with Chemometrics

https://doi.org/10.22146/ijc.36297

Eti Rohaeti(1), Khoirotul Muzayanah(2), Dewi Anggraeni Septaningsih(3), Mohamad Rafi(4*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung, Kampus IPB Dramaga, Bogor 16680, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung, Kampus IPB Dramaga, Bogor 16680, Indonesia
(3) Tropical Biopharmaca Research Center-Institute of Research and Community Services, Bogor Agricultural University, Jl. Taman Kencana No. 3, Kampus IPB Taman Kencana, Bogor 16128, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jl. Tanjung, Kampus IPB Dramaga, Bogor 16680, Indonesia
(*) Corresponding Author

Abstract


Chili is widely used in many food products, especially in Indonesia food. Sometimes a synthetic dye, i.e., rhodamine B or red textile dye were found in commercial chili powder. A fast, precise and accurate analytical method is necessary to authenticate chili powders from the two synthetics dye as they can cause a health problem. A combination of UV-Vis spectroscopy and chemometrics (multivariate analysis) were used in this study to detect rhodamine B and red textile dye present in chili powder samples. Authentication of chili powder from the two dyes by principal component analysis (PCA) with absorbance data from the UV-Vis spectra of samples was not able to classify all of the samples into each group (pure and adulterated chili powder). Fortunately, by using principal component analysis-discriminant analysis (PCA-DA), approximately 89 and 86% of chili samples mixed with rhodamine B and commercial red textile dye were successfully classified into their corresponding group respectively. The combination of UV-Vis spectroscopy with PCA-DA could be used for fast authentication of chili powder from rhodamine B and textile dye used in this study.


Keywords


authentication; chemometrics; chili; synthetic dyes; UV-Vis spectroscopy

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References

[1] Zhigila, D.A., Rahaman, A.A.A., Kolawole, O.S., and Oladele, F.A., 2014, Fruit morphology as taxonomic features in five varieties of Capsicum annuum L. Solanaceae, J Bot., 2014, 540868.

[2] Octaviani, T., Guntarti, A., and Susanti, H., 2014, Penetapan kadar β-karoten pada beberapa jenis cabe (Genus Capsicum) dengan metode spektrofotometri tampak, Pharmaciana, 4 (2), 101–109.

[3] Naccarato, A., Furia, E., Sindona, G., and Tagarelli, A., 2016, Multivariate class modeling techniques applied to multielement analysis for the verification of the geographical origin of chili pepper, Food Chem., 206, 217–222.

[4] Djarismawati, Sugiharti, and Nainggolan, R., 2004, Pengetahuan perilaku pedagang cabai merah giling dalam penggunaan rhodamin B di pasar tradisional di DKI Jakarta, Jurnal Ekologi Kesehatan, 3 (1), 7–12.

[5] Ripaldy, I., Wijanarka, A., and Putriningtyas, N.D., 2017, Analisis kandungan rhodamin B pada cabai merah giling di pasar tradisional di kabupaten Sleman, Daerah Istimewa Yogyakarta, Ilmu Gizi Indonesia, 1 (1), 10–18.

[6] Ellis, D.I., Brewster, V.L., Dunn, W.B., Allwood, J.W., Golovanov, A.P., and Godacre, R., 2012, Fingerprinting food: Current technologies for the detection of food adulteration and contamination, Chem. Soc. Rev., 41 (17), 5706–5727.

[7] Purwakusumah, E.D., Rafi, M., Syafitri, U.D., Nurcholis, W., and Adzkiya, M.A.Z., 2014, Identifikasi dan autentikasi jahe merah menggunakan kombinasi spektroskopi FTIR dan kemometrik, Agritech, 34 (1), 82–87.

[8] Casale, M., Oliveri, P., Armanino, C., Lanteri, S., and Forina, M., 2010, NIR and UV–vis spectroscopy, artificial nose and tongue: Comparison of four fingerprinting techniques for the characterisation of Italian red wines, Anal. Chim. Acta, 668 (2), 143–148.

[9] Sánchez, A.M., Carmona, M., Zalacain, A., Carot, J.M., Jabaloyes, J.M., and Alonso, G.L., 2008, Rapid determination of crocetin esters and picrocrocin from saffron spice (Crocus sativus L.) using uv–visible spectrophotometry for quality control, J. Agric. Food Chem., 56 (9), 3167–3175.

[10] Di Anibal, C.V., Odena, M., Ruisánchez, I., and Callao, M.P., 2009, Determining the adulteration of spices with sudan I-II-II-IV dyes by ultraviolet-visible spectroscopy and multivariate classification techniques, Talanta, 79 (3), 887–892.

[11] Di Anibal, C.V., Rodriguez, M.S., and Albertengo, L., 2014, UV-Visible spectroscopy and multivariate classification as a screening tool to identify adulteration of culinary spices with Sudan I and blends of Sudan I + IV dyes, Food Anal. Methods, 7 (5), 1090–1096.

[12] Boggia, R., Casolino, M.C., Hysenaj, V., Oliveri, P., and Zunin, P., 2013, A screening method based on UV–visible spectroscopy and multivariate analysis to assess addition of filler juices and water to pomegranate juices, Food Chem., 140 (4), 735–741.

[13] Rafi, M., Jannah, R., Heryanto, R., Kautsar, A., and Septaningsih, D.A., 2018, UV-Vis spectroscopy and chemometrics as a tool for identification and discrimination of four Curcuma species, Int. Food Res. J., 25 (2), 643–648.

[14] González-Zamora, A., Sierra-Campos, E., Pérez-Morales, R., Vázquez-Vézquez, C., Gallegos-Robles, M.A., López-Martínez, J.D., and García-Hernández, J.L., 2015, Measurement of capsaicinoids in chiltepin hot pepper: A comparison study between spectrophotometric method and high performance liquid chromatography analysis, J. Chem., 2015, 709150.

[15] Topuz, A., and Ozdemir, F., 2007, Assessment of carotenoids, capsaicinoids and ascorbic acid composition of some selected pepper cultivars (Capsicum annuum L.) grown in Turkey, J. Food Compos. Anal., 20 (7), 596–602.

[16] Rohaeti, E., Rafi, M., Syafitri, U.D., and Heryanto R., 2015, Fourier transform infrared spectroscopy combined with chemometrics for discrimination of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar, Spectrochim. Acta, Part A, 137, 1244–1249.

[17] Gad, H.A., El-Ahmady, S.H., Abou-Shoer, M.I., and Al-Azizi, M.M., 2012, Application of chemometrics in authentication of herbal medicines: A review, Phytochem. Anal., 24 (1), 1–24.

[18] Kaiser, H.F., 1960, The application of electronic computers to factor analysis, Educ. Psychol. Meas., 20 (1), 141–151.



DOI: https://doi.org/10.22146/ijc.36297

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