Determination of Stevioside and Rebaudioside A in  Stevia rebaudiana  Bertoni Leaves Using near Infrared Spectroscopy and Multivariate Data Analysis

Yohanes Martono; Suryasatriya Trihandaru; Ferdy Semuel Rondonuwu

doi:10.22146/ijc.25580

Determination of Stevioside and Rebaudioside A in Stevia rebaudiana Bertoni Leaves Using near Infrared Spectroscopy and Multivariate Data Analysis

https://doi.org/10.22146/ijc.25580

Yohanes Martono⁽¹⁾, Suryasatriya Trihandaru⁽²⁾, Ferdy Semuel Rondonuwu^(3*)

(1) Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Jl. Diponegoro 52-60, Salatiga 50711, Indonesia
(2) Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Jl. Diponegoro 52-60, Salatiga 50711, Indonesia
(3) Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Jl. Diponegoro 52-60, Salatiga 50711, Indonesia
(*) Corresponding Author

Abstract

Rebaudioside A and stevioside are abundant steviol glycoside contained in Stevia rebaudiana leaves. These components are widely used as a natural sweetener. The objective of this study was to develop rapid determination method of stevioside, and rebaudioside A in S. rebaudiana leaves using near infrared trans-reflectance spectroscopy (NIRS) combined with multivariate analysis. The reference method used was HPLC. A prediction model was developed using partial least square (PLS) regression. Calibration parameters were calculated based on a calibration set of various stevioside, rebaudioside A from 23 samples. Performance of PLS model was assessed in term of optimum determination coefficient (R²), and minimum root mean square error of cross-validation (RMSEV). Validation of PLS model was performed using cross-validation and leave one out calibration of PLS component. Rebaudioside A has well PLS model in wavenumber region of 4100–5100 cm^-1, and stevioside determination using difference wavenumber region of 4760-5016 cm^-1. PLS model for total (sum of stevioside and rebaudioside A content) was exploited in wavenumber region of 4568-4928 cm^-1. NIRS in combination with multivariate data analysis of PLSR can be applied as a rapid method for determining rebaudioside A and the total amount of steviol glycosides in S. rebaudiana leaves.

Keywords

near infrared spectroscopy; PLSR; stevioside; rebaudioside A; Stevia rebaudiana

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DOI: https://doi.org/10.22146/ijc.25580