Isolation and Identification of Volatile Components in Tempe by Simultaneous Distillation-Extraction Method by Modified Extraction Method

Syahrial Syahrial(1), M. Muchalal(2*)

(1) Department of Chemistry Faculty of Mathematics, Syiah Kuala University, Banda Aceh
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
(*) Corresponding Author


An isolation and identification of volatile components in temps for 2, 5 and 8 days fermentation by simultaneous distillation-extraction method was carried out. Simultaneous distillation-extraction apparatus was modified by Muchalal from the basic Likens-Nickerson's design. Steam distillation and benzena as an extraction solvent was used in this system. The isolation was continuously carried out for 3 hours which maximum water temperature In the Liebig condenser was 8 °C. The extract was concentrated by freeze concentration method, and the volatile components were analyzed and identified by combined gas chromatography-mass spectrophotometry (GC-MS). The Muchalal's simultaneous distillation extraction apparatus have some disadvantage in cold finger condenser, and it's extractor did not have condenser. At least 47, 13 and 5 volatile components were found in 2, 5 and 8 days fermentation, respectively. The volatile components in the 2 days fermentation were nonalal, ɑ-pinene, 2,4-decadienal, 5-phenyldecane, 5-phenylundecane, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane, 5-phenyltridecane, and caryophyllene; in the 5 days fermentation were nonalal, caryophyllene, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane; and in the 8 days fermentation were ethenyl butanoic, 2-methy1-3-(methylethenyl)ciclohexyl etanoic and 3,7-dimethyl-5-octenyl etanoic.


tempe; Muchalal's simultaneous distillation-extraction apparatus

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[1]   Fennema, Owen R., eds., 1971, Flavour Research; Principles and Techniques, Marcel Dekker, Inc., New York.

[2]   Heath, Henry, B., Reineccius, Gary, 1986, Flavour Chemistry and Technology, AVI Publishing Co., Inc., Wesport, Connecticut.

[3]   Kasmidjo, R.B., 1990, Tempe, Mikrobiologi Biokimia Pengolahan serta Pemanfaatannya, PAU Pangan dan Gizi UGM, Yogyakarta.

[4]   Kubota, Kikkue, Kobayashi, Akio, 1988, Identification of Unknown Methyl Ketones in Volatile Flavor Components from Cooked Small Shrimp, J. Agric. Food Chem., 36, 121-123.

[5]   Murata, Kiku, Ikehata, Hideo, Miyamoto, Teijiro, 1967, Studies on the Nutritional Value of Tempeh, Journal of Food Science, 32, 580-586.

[6]   Nout, M.J.R., Rombouts, F.M., Review Recent Developments in Tempe Research, J. ApII. Bac.

[7]   Rao, Alapati Srinivasa, Rajanikant, Bandaru, Seshadri, Ramachandran., 1989, Volatile Aroma Components of Curcuma amada Roxb., J. Agric. Food Chem., 37, 740-743.

[8]     Schieberfe, Peter, Grosch, VVOrner, 1988, Identification of Potent Flavour Compounds Formed in an Aqueous Lemon Oil/Citric Acid Emulsion, J. Agric. Food Chem., 38, 797-800.

[9]     Sudarmin, 1996, Analisa Senyawa Flavor/Senyawa Residu pads Tempe Selama Penyimpanan, Laporan Magang PenelNan, Laboratorium Kimia Organik FMIPA UGM Yogyakarta.

[10]   Stenkraus, Keith H., 1983, Handbook of Indigenous Fermented Foods, Marcel Dekker Inc., New York.

[11] Yu, Tung-His, Wu, Chung-May, Liou, Yoh-Chemg, 1989, Volatile Compounds from Garlic, J. Agric. Food Chem., 37, 725-730.

[12] Yu, Tung-His, Wu, Chung-May, Chen, Shih-Yuan, 1989, Volatile Compounds from Garlic, J. Agric. Food Chem., 37, 725-730.

[13] Wu, Chung-May, dkk, 1987, Volatile Compounds of the Wax Gourd (Benincasa hispida, Cogn) and a Wax Gourd Beverage, Journal of Food Science, 52, 1, 132-134.

[14] Zapsalis, Charles dan Beck, R, R. Anderle, 1985, Food Chemistry and Nutritional Biochemistry, John Willey and Sons, New York.

[15] Bentz, H,D., Grosch, W., 1987, Food Chemistry, Springer-Verlag, Berlin.

[16] Bessiere, Y., Thomas, A.F., eds., 1990, Flavour Science and Technology, John Willey & Sons, Chicester.

[17]   BPPT, 1986, Tempeh Fermentation, BPPT, Jakarta.


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