CALLUS INDUCTION AND PHYTOCHEMICAL CHARACTERIZATION OF Cannabis sativa CELL SUSPENSION CULTURES

https://doi.org/10.22146/ijc.21776

Tri Joko Raharjo(1*), Ogbuadike Eucharia(2), Wen-Te Chang(3), Robert Verpoorte(4)

(1) Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta
(2) Division of Pharmacognosy, Section Metabolemics, Institute of Biology, Leiden University, Gorlaeus Laboratories, PO Box 9502, 2300 RA, Leiden, The Netherlands
(3) Department. of Applied Plant Sciences, TNO Voeding, Zernikedreef 8, Leiden, The Netherlands
(4) Division of Pharmacognosy, Section Metabolemics, Institute of Biology, Leiden University, Gorlaeus Laboratories, PO Box 9502, 2300 RA, Leiden, The Netherlands
(*) Corresponding Author

Abstract


Callus of Cannabis sativa has been successfully induced from C. sativa explants and seedings. It seems that flowers are the best explant for callus induction and induction under light also give better results than induction in dark. Four cell culture lines were established from flower induced callus. Phytochemical profiles of C. sativa suspension cell cultures were investigated using HPLC and 1H-NMR. Cannabinoids and phenolic compounds related to cannabinoids such as flavonoids could not be found in the cell suspension cultures and there is no major chemical difference between the cell lines though they can visually be distinguished by their colors. Only in one cell line some aromatic compounds in the water/methanol extract could be observed in the 1H-NMR. Further investigations showed that none of these compounds are flavonoids. It seems that lack of cannabinoids in the cell cultures is related to lack of polyketide synthase activity.


Keywords


Callus; Cannabis; phytochemical

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References

[1] Kutchan, T.M., Hampp, N., Lottspeich, F., Beyreuther, K., and Zenk, M.H., 1988, FEBS Lett, 237, 40-44.

[2] De Luca, V., Marineau, C., and Brisson, N., 1989, Proc Natl Acad Sci USA, 86, 2582-2586

[3] Pasquali, G., Goddijn, O.J.M., de Waal, A., Verpoorte, R., Schilperoort, R.A., Hoge, J.H.C., and Memelink, J., 1992, Plant Molecular Biol. 18, 1121-1131.

[4] Loh, W.H.T, Hartsel, S.C. and Robertson, L.W., 1983, Z Pflanzenphysiol Bd, 111, S.395-400

[5] Hartsel, S.C., Loh, W.H.T., and Robertson, L.W., 1983, Planta Med., 48, 17-19

[6] Veliky, I.A., and Genest, K., 1972, J Nat Prod, 35, 450-456

[7] Heitrich, A., and Binder, M., 1982, Experentia, 38, 898-899

[8] Itokawa, H., Takeya, K., and Mihashi, S., 1977, Chem Pharm Bull, 25, 1941-1946

[9] Turner, C.E., Elsohly, M.A. and Boeren, E.G., 1980, J Nat Prod, 43,169-234

[10] Murashige, T., and Skoog, F., 1962, Physiol Plant, 15, 473-497

[11] Casas, E., 2003, Metabolic Profiling of Catharanthus roseus Leaves Infected by Pythoplasma using 1H-NMR and Principle Component Analysis, Departament de Productes Naturalis Facultat de Farmacia, Universitat de Barcelona. Barcelona.

[12] Mabry, T.J., Markham, K.R., and Thomas, M.B., 1970, The Systematic Identification of Flavonoids, Springer-Verlag, Berlin

[13] Pouchert, C.J., 1983, The Aldrich Library of NMR Spectra, 2 ed., Vol. 2. Aldrich Chemical Company Inc. Milwaukee



DOI: https://doi.org/10.22146/ijc.21776

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