BIOSURFACTANTS PRODUCTION BY Pseudomonas aeruginosa USING SOYBEAN OIL AS SUBSTRATE

Venty Suryanti; Sri Hastuti; Tutik Dwi Wahyuningsih; Mudasir Mudasir; Dina Ika Muliawati

doi:10.22146/ijc.21570

BIOSURFACTANTS PRODUCTION BY Pseudomonas aeruginosa USING SOYBEAN OIL AS SUBSTRATE

https://doi.org/10.22146/ijc.21570

Venty Suryanti^(1*), Sri Hastuti⁽²⁾, Tutik Dwi Wahyuningsih⁽³⁾, Mudasir Mudasir⁽⁴⁾, Dina Ika Muliawati⁽⁵⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A Surakarta 57126 Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A Surakarta 57126 Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Gadjah Mada University Sekip Utara, P.O Box Bls. 21 Yogyakarta 55281 Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Gadjah Mada University Sekip Utara, P.O Box Bls. 21 Yogyakarta 55281 Indonesia
(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A Surakarta 57126 Indonesia
(*) Corresponding Author

Abstract

Optimization condition of the biosurfactants production by P. aeruginosa using soybean oil as substrate has been examined. The media containing 10% v/v of the soybean oil and 6 days of the fermentation time was the optimum condition for the biosurfactants production. The extraction technique using different solvent polarity (n-hexane, chloroform, ethyl acetate and buthanol, respectively) was applied for the isolation of the biosurfactants. The biosurfactant was found in the extract chloroform of the crude biospasoy (biosurfactants obtained from soybean oil as substrate) which then is called chlo-biospasoy. The chlo-biospasoy was identified as rhamnolipids which had oil in water (o/w) emulsion type, had the CMC of 860 mg/L and could reduced the surface tension of the water from 72 mN/m to 52 mN/m. The chlo-biospasoy could be used as an emulsifier to form emulsion between water and hydrocarbon such as palm oil, benzene, premium or toluene with various stability. The results indicated that chlo-biospasoy could be used as an emulsifying and emulsion-stabilizing agent.

Keywords

Biosurfactants; P. aeruginosa; Soybean Oil; Emulsifier

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References

[1] Mulligan, C.N., Yong, T.N., Gibbs, B.F., 2001, Environmental Application for Biosurfactants. Environ. Poll., 133 (2), 183.

[2] Desai, J.D. and Banat, I.M., 1997, Microbial Production of Surfactants and Their Commercial Potential, Microbiol. Mol. Rev., 61, 47-64.

[3] Fiechter, A., 1992, Biosurfactans: Moving Toward Industrial Application, Trends in Biotech., 10, 208-217.

[4] Ghazali, R. and Ahmad, S., 1997, Biosurfactans-A Review, Elaeis, 9 (1),34-54.

[5] Sasidharan, V., Thambirajah, J.J., Ho, C.C., and Hasyim, M.A., 1993, Microbial Production of Biosurfactans From Palm Oil by Candida bombicola, The 16'^hMalaysian Microbiology Symposium, 62-64.

[6] Ferraz, C., De Araujo, A.A., and Postore, G.M., 2002, The Influence of Vegetable Oils on Biosurfactant Production by Serratia marcescens, Appl. Biochem. Biotechnol., 98-100:841-847.

[7] Schroepfer, J., 1965, Enzymatic Stereospecificity in The Conversion of Oleic Acid to 10-Hydoxystearic Acid, J. Am. Oil Chem. Soc., 87, 1411-1412.

[8] Schroepfer, J., 1966, Stereospecific Conversion of Oleic Acid to 10-Hydoxystearic Acid, J. Biol. Chem., 241, 5441-5447

[9] Wallen, L.L., Benedict, R.G., and Jackson, R.W., 1962, The Microbiological Production of 10-ydroxystearic Acid from Oleic Acid, Arch. Biochem. Biophy, 99, 249-253

[10] Mercade, E., Robert, M., Espuny, M.J., Bosch, M.P., Manresa, M.A., Parra, J.L., and Uinea, J., 1988, New Surfactant Isolated from Pseudomonas 42A2, J. Am. Oil. Chem. Soc., 65, 1915-1916

[11] Hou, C.T., Bagby, M.O., Plattner, R.D., and Koritala, S., 1991, A Novel Compound, 7,10-Dihydroxy-8-(E)-Octadecenoic Acid from Oleic Acid by Bioconversion, J.Am.Oil Chem.Soc., 68, 99-101.

[12] Hou, C.T. and Bagby, M.O., 1991, Microbial Production of A Novel Compound, 7,10-Dihydroxy-8-(E)-Octadecenoic Acid, PCT/US91/ 01806.

[13] Solomons, T.W.G., 1992, Organic Chemistry, John Wiley & Sons, Inc., USA

[14] Tuleva, B.J., Ivanov, G.R., and Christova, N.E., 2002, Biosurfactant Production by a New Pseudomonas putida Strain, Z. Naturforsch, 57c, 356-360.

[15] Bodour, A.A., Barajas, C.G., Jiorle, B.V., Malcomson, M.E., and Paull, A.K., 2004, Structure and Characterization of Flavolipids, a Novel Class of Biosurfactants Produced by Flavobacterium sp. Strain MTN11, Appl. Environ. Microbiol., 70 (1): 114-120.

[16] Bodour, A.A., and Miller-Maier, R.M., 1998, Application of a modified drop-collapse technique for surfactant quantification and screening of biosurfactant-producing microorganisms, J. Microbiol. Methods 32: 273-280.

[17] Zhang, Y. and Miller, R.M., 1992, Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant)., Appl. Environ. Microbiol. 58, 3276-3282.

[18] Kim, J.S., M. Powalla, S., Lang, F., Wagner, H., Lunsdorf, L., and Wray, V., 1990, Microbiol glycolipid production under nitrogen limitation and resting cell conditions., J. Biotechnol., 13, 257-266.

[19] Rapp, P., Bock, H., Wray, V., and Wagner, F., 1979, Formation, Isolation and Characterization of Trehalose Dimycolates from Rhodococcus erythropolis Grown on n-alkales., J. Gen. Microbiol., 115, 491-503.

[20] Kim, S.H., Lim, E.J., Lee, S.O., Lee, J.D., and Lee, T.H., 2000, Purification and Characterization of Biosurfactants form Nocardia sp. L-417, Biotech. Appl. Biochem., 31: 249-253.

[21] Lee, S.C., Jung, Y.J., Yoo, J.S., Cho, Y.S., Cha, I.H., and Choi, Y.L., 2002, Characteristic of Biosurfactants Produced by Bacillus sp. LSC11, Kor. J. LifeSci. 12: 745-751.

[22] Suk, W.S., Son, H.J., Lee, G., and Lee, S.J., 1999, Purification and Characterization of Biosurfactants Produced by Pseudomonas sp. SW1, J. Microbiol. Biotechnol., 9: 56-61.

[23] Parkinson, M., 1985, Biosurfactants, Biotechnol. Adv., 3, 65-83

DOI: https://doi.org/10.22146/ijc.21570