ANTIBIOTIC COMPOUND FROM MARINE ACTINOMYCETES (Streptomyces sp A11): ISOLATION AND STRUCTURE ELUCIDATON

https://doi.org/10.22146/ijc.21464

Rofiq Sunaryanto(1*), Bambang Marwoto(2), Tun Tedja Irawadi(3), Zainal Alim Mas’ud(4), Liesbetini Hartoto(5)

(1) Center of Biotechnology BPPT PUSPIPTEK Serpong Tangerang Selatan Banten 15340; Graduate School of Agroindustry Technology, Bogor Agriculture University Kampus IPB Dermaga, Bogor Jawa Barat
(2) Center of Biotechnology BPPT PUSPIPTEK Serpong Tangerang Selatan Banten 15340
(3) Graduate School of Agroindustry Technology, Bogor Agriculture University Kampus IPB Dermaga, Bogor Jawa Barat
(4) Graduate School of Agroindustry Technology, Bogor Agriculture University Kampus IPB Dermaga, Bogor Jawa Barat
(5) Graduate School of Agroindustry Technology, Bogor Agriculture University Kampus IPB Dermaga, Bogor Jawa Barat
(*) Corresponding Author

Abstract


Purification and structure elucidation of antibiotic produced by marine actinomycetes (Streptomyces sp A11) was conducted. Production of antibiotic was carried out by liquid fermentation using yeast and peptone medium for 5 days fermentation. Purification of antibiotic was carried out by silica gel 60 (Merck, 0.063-0.200 mm) column chromatography and preparative HPLC. Structure elucidation was carried out using ESI-MS, 1H NMR, 13C NMR, DEPT 13C NMR, and FTIR. This antibiotic was identified as cyclo (tyrosyl-prolyl) / (C14H16N2O3). This antibiotic had biological activity to Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC 66923, Pseudomonas aeruginosa ATCC27853, and produced by extracellular secretion.


Keywords


antibiotic; actinomycetes; purification; structure elucidation

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DOI: https://doi.org/10.22146/ijc.21464

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