DIETARY ADENINE ALLEVIATES FATTY LIVER INDUCED BY OROTIC ACID

https://doi.org/10.22146/ijc.21444

Yohanes Buang(1*)

(1) Laboratory of Applied Biochemistry, Department of Applied Biological Science, Saga University, Saga Shi, Honjo Machi-1, Saga
(*) Corresponding Author

Abstract


The effects of dietary adenine in fatty liver induced by orotic acid (OA) were studied. Rats were paired-fed 1% OA-supplemented diets with/or without 0.25% adenine or a diet without OA for 10 days. Serum lipid profiles were measured using enzyme assay kits. Lipids of liver tissues were extracted and liver lipid contents were determined. A peach of liver was prepared to determine the activities of fatty acid synthase (FAS) and fatty acid β-oxidation. The results showed that liver TG content of OA-fed rats increased markedly in comparison to basal group.  However, the addition of adenine to the diet reversed promotion of liver TG content to basal level. It was also found that FAS activities decreased. Furthermore, these diets reversed the inhibition of fatty acid β-oxidation to basal level and induced the serum lipid levels secretion. Therefore, the alleviation of fatty liver in OA-treated rats given dietary adenine is associated with the inhibition of FAS activities accompanied with the promotion of mitochondrial fatty acid β-oxidation and the promotion of serum lipid secretion from the hepatic tissue into the bloodstream.

Keywords


adenine; orotic acid; serum lipid; triglyceride; fatty liver; hepatic steatosis

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References

[1] Li, Z., Clark, J., and Diehl, A.M., 2002, Clin. Liver Dis., 6, 4, 867–877.

[2] Harrison, S.A., and Diehl, A.M., 2002, Semin. Gastrointest. Dis., 13, 1, 3–16.

[3] Website. http://www.patient.co.uk/health/Fatty-Liver-Disease.htm. Accessed July 10, 2009.

[4] Sherlock, S., and Dooley, J., 1997, Diseases of the liver and biliary system, Blackwell Science, Oxford.

[5] Raisonnier, A., Bouma, M.E., Salvat, C., and Ifante, R., 1981, Eur. J. Biochem., 118, 3, 565-569.

[6] Miyazawa, S., Furuta, S., and Hashimoto, T., 1982, Biochim. Biophys. Acta, 711, 3, 494–502.

[7] Cha, J.Y., Cho, Y.S., Kim, I., Anno, T., Rahman, S.M., and Yanagita, T., 2001, Plant Foods Hum. Nutr., 56, 4, 349–358.

[8] Lehninger, A.L., Nelson, D.L., and Cox, M.M., 1993, Principles of Biochemistry, 2nd ed., Worth Publishers, Inc. USA.

[9] Dixon, J.B., Bhathal, P.S., and O’Brien, P.E., 2001, Gastroenterology, 121, 1, 91–100.

[10] Schwimmer, J.B., Deutsch, R., Rouch, J.B., Behling, C., Newbury, R., and Lavine, J.J., 2003, J. Pediatr., 143, 4,. 500–505.

[11] Cotrim, H.P., Paraná, R., Braga, E., and Lyra, L., 2000, Am. J. Gastroenterol., 95, 10, 3018–3019.

[12] Rolo, A.P., Teodoro, J.S., Peralta, C., Rosello-Catafau, J., and Palmeira, C.M., 2009, Transpl. Int.., 22, 11, 1081–1090.

[13] Tilg, H., and Hotamisligil, G.S., 2006, Gastroenterology, 131, 3, 934–945.

[14] Pagano, C., Soardo, G., Pilon, C., Milocco, C., Basan, L., Milan, G., Donnini, D., Faggian, D., Mussap, M., Plebani, M., Avellini, C., Federspil, G., Sechi, L.A., and Vettor, R., 2006, J. Clin. Endocrinol. Metab., 91, 3,1081–1086.

[15] Tilg, H., and Moschen, A.R., 2008, Mol. Med., 14, 3-4, 222–231.

[16] Buang, Y., Wang, Y.M., Cha, J.Y., Nagao, K., and Yanagita, T., 2005, Nutrition, 21, 7-8, 867–873.

[17] Buang, Y., Cha, J.Y, Nagao, K., Wang, Y.M., Inoue, N., and Yanagita, T., 2004, J. Nutr. Sci. Vitaminol., 50, 4 272–276.

[18] Folch, J., Lees M., and Sloane-Starley, G.H., 1957, J. Biol. Chem., 226, 1, 497–509.

[19] Fletcher, M.J., 1968, Clin. Chim. Acta, 22, 3, 393–397.

[20] Barlett, G.R., 1959, J. Biol. Chem., 234, 466–468.

[21] Nagao, K., Inoue, N.,Wang, Y.M.,and Yanagita, T., 2005, J. Nutr., 135, 9–13.

[22] Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randal, R.J., 1951, J. Biol. Chem., 193, 1, 265–275.

[23] Duncan, D.B., 1955, Biometric, 11, 1–42.

[24] Jou, J., Choi, S.S., and Diehl, A.M., 2008, Semin. Liver Dis., 28, 4, 370–379.

[25] Rutledge, A.C., and Adeli K., 2007, Nutr. Rev., 65, S13–23.

[26] Ahmed, M.H., and Byrne, C.D., 2007, Drug Discov. Today, 12:740-747.

[27] Matsuzaka, T., Shimano, H., Yahagi, N., Amemiya-Kudo, M., Okazaki, H., Tamura, Y., Iizuka, Y., Ohashi, K., Tomita, S., Sekiya, M., Hasty, A., Nakagawa, Y., Sone, H., Toyoshima, H., Ishibashi, S., Osuga, J., and Yamada, N., 2004, Diabetes, 53, 3, 560–569.

[28] Horton, J.D., Goldstein, J.L., and Brown, M.S., 2002, J. Clin. Invest., 109, 9, 1125–1131.

[29] Hu, X.Q., Wang, Y-M., Wang, J-F., Xue, Y., Li, Z-J., Nagao, K., Yanagita, T., and Xue, C-H. , 2010, Lipids in Health and Disease, 9, 25, 1-9.

[30] Windmueller, H.G., 1964, J. Biol. Chem., 239, 2, 530–537.

[31] Stipanuk, M.H. 2006, Biochemical, Physiological, and Molecular Aspects of Human Nutrition, 2nd ed., Saunders-Elsevier, New York, 666–672.

[32] Pottenger, L.A., and Gets, G.S., 1971, J. lipid Res., 12, 450–459.

[33] Viollet, B., Foretz, M., Guigas, B., Horman, S., Dentin, R., Bertrand, L., Hue, L., and Andreelli, F., 2006, J. Physiol., 574, 1, 41–53.

[34] Yu, V.Y.H, 1998, HK J. Paediatr., 3,122-126.

[35] Karasawa Y., Takasaki, K., and Koh, K., 2002, J. Poult. Sci., 39, 4, 285–291.

[36] Ventura, F.V., Tavares, D.A.I., and Wanders R.J.A., 2007, Biochem. Biophys. Res. Commun., 352, 4, 873–878.

[37] den Boer, M.E.J., Wanders, R.J.A., Morris, A.A.M., IJlst, L., Heymans, H.S.A., and Wijburg, F.A., 2002, Pediatrics, 109, 1, 99–104.



DOI: https://doi.org/10.22146/ijc.21444

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