ADSORPTION OF AFLATOXIN B1 IN CORN ON NATURAL ZEOLITE AND BENTONITE

https://doi.org/10.22146/ijc.21343

Nuryono Nuryono(1*), Ali Agus(2), Sri Wedhastri(3), Y.M.S. Maryudhani(4), Deni Pranowo(5), Yunianto Yunianto(6), Ebrahim Razzazi-Fazeli(7)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(2) Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
(6) Animal Husbandry Academy Brahma Putra, Yogyakarta
(7) Proteomic Unit, VetCore Facility for Research, University of Veterinary Medicine, Vienna
(*) Corresponding Author

Abstract


A study on adsorption of AFB1 in corn (kernel and grained) on natural zeolite and bentonite has been investigated. The first work was adsorption in a batch system of standard AFB1 solution on adsorbents. Some factors such as contact time, concentration of AFB1 and particle size of adsorbent were evaluated. The amount of AFB1 adsorbed was calculated based on the difference of AFB1 concentration before and after adsorption determined by high performance liquid chromatography (HPLC) method. Adsorption of AFB1 in corn sample was emphasized by mixing aqueous suspension of sample with adsorbent. Concentration of AFB1 in suspension was analyzed by enzyme-linked immuno-sorbent assay (ELISA) method. Result shows that adsorption of AFB1 on adsorbents of natural zeolite and bentonite is very fast. Within 15 min 99% of AFB1 (200 ng/mL) has been adsorbed by 25 mg of bentonite and 96% by zeolite. The particle size higher than 200 mesh did not give significant effect on the AFB1 adsorption capability. Effectiveness of zeolite in adsorbing AFB1 is lower than that of bentonite. Capability in reducing AFB1 contamination in corn samples (kernel and meal) for both adsorbents is lower than that in standard solution.

Keywords


aflatoxin; corn; adsorption; bentonite; zeolite

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References

[1] Abdel-Wahhab, M.A., Nada, S.A., and Amra, H.A., 1999, J. Appl. Toxicol., 19, 3, 199–204.

[2] Abdel-Wahhab, M.A., and Aly, S.E., 2003, J. Agric. Food Chem. 51, 8, 2409–2414.

[3] Park, J.W., Kim, E.K., Shon, D.H., and Kim, Y.B., 2002, Food Addit. Contam., 19, 11, 1073–1080.

[4] Ali, N., Sardjono, Yamashita, A., and Yoshizawa, T., 1998, Food. Addit. Contam., 15, 4, 377–384.

[5] Noviandi, C.T., Razzazi, E., Agus, A., Böhm, J., Hulan, H.W., Wedhastri, S., Maryudhani, Y.B., Nuryono, Sardjono, and Leibetseder, J., 2001, Mycotoxin Res., 17A, 2, 174–177.

[6] Bahri, S., Ohim, and Maryam, R., 1995, Residu aflatoksin M1 pada susu sapi dan hubungannya dengan keberadaan afaltoksin B1 pada pakan sapi, Kumpulan Makalah Lengkap Kongres Nasional Perhimpunan Mikologi Kedokteran Manusia dan Hewan Indonesia I dan Temu Ilmiah, Bogor, 21-24 Juli 1994, 269–275

[7] Nuryono, Agus, A., Wedhastri, S., Maryudani, Y.B., Sigit Setyabudi, F.M.C., Böhm, J., and Razzazi-Fazeli, E., 2009, Food Control, 20, 8, 721–724.

[8] Maryam, R., Bahri, S., and Zahari, P., 1994, Deteksi aflatoksin B1, M1 dan Aflatoksikol dalam Telur dengan Kromatografi Cair Kinerja Tinggi, Prosiding Teknologi Veteriner untuk Kesehatan Hewan dan Keamanan Pangan, Bogor, 22-24 Maret 1994.

[9] Maryam, R., 1996, Residu Aflatoksin dan Metabolitnya dalam daging dan Hati Ayam, Prosiding Temu Ilmiah Nasional Bidang Veteriner, Bogor, 12-13 Maret 1996, 236–339.

[10] Hall, J.A., and Wild, P.C., “Epidemiology of aflatoxins related disease”, in The toxicology of aflatoxins, L.D. Eaton and J.D. Groopman ed., London: Academic Press, Inc., 1994, 233–258.

[11] Sudjadi, S., Machmud, M., Damardjati, D.S., Hidayat, A., Widowati, S., and Widiati, A. 1999. Aflatoxin research in Indonesia. Elimination of Aflatoxin Contamination in Peanut, Australian Centre for International Agricultural Research, Canberra, 23–25.

[12] Anderson, R.A., “Detoxification of aflatoxin contaminated corn” in Aflatoxin and Aspergillus flavus in Corn Southern Cooperative Series Bulletin 279, Diener, U., Asquith, R., Dickens, J. ed., Auburn University, Auburn, AL, 1983, 87–90.

[13] Bintvihok, A., 2002, Feed Tech., 6, 1, 28–29.

[14] Phillips, T.D., Kubena, L.F., Harvey, R.B., Taylor, D.R., and Heidelbaugh, N.D., 1988, Poult. Sci., 67, 2, 243–247.

[15] Abdel-Wahhab, M.A., Nada, S.A., and Khalil, F.A., 2002, Animal Feed Sci. Technol., 97, 3, 209–219.

[16] Aly, S.E., Abdel-Galil, M.M., and Abdel-Wahhab, M.A., 2004, Food Chem. Toxicol., 42, 11, 1825–1831.

[17] Desheng, Q., Fan, L., Yanhu, Y., and Niya, Z., 2005, Poult. Sci., 84, 6, 959–961.

[18] Thieu N.Q., and Pettersson, H., 2008, Mycotoxin Res., 24, 3, 124-129.

[19] Gallo, A., and Masoero, F., 2010, Ital. J. Anim. Sci., 9, 1, 109–116.

[20] Ramesh, A., Hasegawa, H., Sugimoto, W., Maki, T., and Ueda, K., 2008, Bioresour. Technol., 99, 9, 3801–3809.

[21] Daković, A., Tomasević-Canović, M., Dondur, V., Rottinghaus, G.E., Medaković, V., and Zarić, S., 2005, Colloids Surf., B, 46, 1, 20–25.

[22] Arvide, M.G.T., Mulder, I., Velazquez, A.L.B., and Dixon, J.B., 2008, Clays Clay Miner., 56, 5, 571–578.



DOI: https://doi.org/10.22146/ijc.21343

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