Ameliorative Synergistic Effect of Honey and MO on Lead-Induced Alteration of Biochemical and Haematological Indices in Clarias gariepinus

https://doi.org/10.22146/ifnp.77925

Tolulope Ayandiran(1), Adebola Akintola(2), Busuyi David Kehinde(3*), Ogundola Adijat(4), Fawole Olatunde(5), Akwu Bala Peter(6), Akitunde Akinniyi(7), Folorunso Kolade(8)

(1) Department of Biology, Ladoke Akintola University of Technology
(2) Department of Science Laboratory Technology, Ladoke Akintola University of Technology
(3) Ladoke Akintola University of Technology
(4) Department of Biology, Ladoke Akintola University of Technology
(5) Department of Biology, Ladoke Akintola University of Technology
(6) Department of Anatomy, Kogi State University
(7) Department of Biochemistry, Ladoke Akintola University of Technology
(8) Department of Anatomy, Ladoke Akintola University of Technology, Nigeria
(*) Corresponding Author

Abstract


Lead confers deleterious damage to the cells and it is necessary to explore further and develop a more effective way to ameliorate lead toxicity. This study aims to investigate how honey and Moringa oleifera (MO) can synergistically provide a more effective way to ameliorate lead toxicity. Groups of ten fish (Clarias gariepinus) were given Pb (0.30 g) and supplemented feed (T1) containing both honey (5 g) and MO (5 g). Others received pb (0.30 g) and supplemented feed (T2) containing either honey (10 g) or (T3) containing MO (10 g). Finally, hematological and biochemical analysis were conducted and a decline in the hematological parameters was observed. Also, the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea, creatinine, and malondialdehyde (MDA) significantly increased while glutathione (GSH) and superoxide dismutase (SOD) reduced in the group given lead only. However, these biochemical indices and hematological parameters are greatly restored in the group that received both honey and MO compared to the group that received either honey or MO. The findings of this study reveal that co-administration of both honey and MO synergistically damages caused by lead-induced toxicity better.


Keywords


Synergistic; Biochemical; Haematological; Honey; Moringa oleifera(MO); Clarias gariepinus

Full Text:

PDF


References

Abdel-Mohsien, H. S. and Mahmoud, M. A. M. (2015). Accumulation of Some Heavy Metals in <i>Oreochromis niloticus</i> from the Nile in Egypt: Potential Hazards to Fish and Consumers’, Journal of Environmental Protection. https://doi.org/10.4236/jep.2015.69089.

Acuram, L. K. and Chichioco Hernandez, C. L. (2019) Anti-hypertensive effect of Moringa oleifera Lam, Cogent Biology, 5(1). https://doi.org/10.1080/23312025.2019.1596526.

Adedapo, A. A., Mogbojuri, O. and Emikpe, B. (2009). Safety evaluations of the aqueous extract of the leaves of Moringa oleifera. Journal of Medicinal Plants Research, 3(8), 586-591.

Adekola, F. A. and Eletta, O. A. A. (2007). A study of heavy metal pollution of Asa River, Ilorin. Nigeria; trace metal monitoring and geochemistry. Environmental Monitoring and Assessment, 125(1-3), 157-63 https://doi.org/10.1007/s10661-006-9248-z.

Ademuyiwa, O. et al. (2010). Effects of sub-chronic low-level lead exposure on the homeostasis of copper and zinc in rat tissues, Journal of Trace Elements in Medicine and Biology. https://doi.org/10.1016/j.jtemb.2010.01.002.

Aebi, H.E. (1983) Catalase. In: Bergmeyer, H.U., Ed., Methods of Enzymatic Analysis, Verlag Chemie, Weinhem.

Bandyopadhyay, D. et al. (2014). Lead induced oxidative stress: a health issue of global concern. Journal of Pharmacy Research, 88, 1198-1207.

Bijoor, A. R., Sudha, S. and Venkatesh, T. (2012) Neurochemical and neurobehavioral effects of low lead exposure on the developing brain, Indian Journal of Clinical Biochemistry. https://doi.org/10.1007/s12291-012-0190-2.

Corallo, A., Lazoi, M. and Lezzi, M. (2020). Cybersecurity in the context of industry 4.0: A structured classification of critical assets and business impacts. Computers in Industry, 114, 103-165. https://doi.org/10.1016/j.compind.2019.103165.

Dacie, J.V and Lewis S.M. (1975) Pratical Haematology. Heinemann. pp 277-284

El-Guendouz, S. et al. (2017). Antioxidant and diuretic activity of co-administration of Capparis spinosa honey and propolis in comparison to furosemide, Asian Pacific Journal of Tropical Medicine, 10(10), 974–980. https://doi.org/10.1016/j.apjtm.2017.09.009.

ElKoshairy, N., Hassan, R. and Halawa, A. (2014). The effect of lead toxicity on albino rats’ filiform and fungiform papillae and the possible protective role of honey and black seed. Journal of Environmental and Occupational Science, 3(2), 87. https://doi.org/10.5455/jeos.20140328072527.

Fakurazi, S., Hairuszah, I. and Nanthini, U. (2008) Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level, Food and Chemical Toxicology, 46(8), 2611–2615. https://doi.org/10.1016/j.fct.2008.04.018.

Fihri, A. F. et al. (2016). Protective Effect of Morocco Carob Honey Against Lead-Induced Anemia and Hepato-Renal Toxicity. Cellular Physiology and Biochemistry, 39(1), 115–122. https://doi.org/10.1159/000445610.

Gupta, R. et al. (2005). Therapeutic effects of Moringa oleifera on arsenic-induced toxicity in rats, Environmental Toxicology and Pharmacology, 20(3), 456–464. https://doi.org/10.1016/j.etap.2005.05.005.

Hissin, P. J., & Hilf, R. (1976). A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analytical biochemistry, 74(1), 214–226.

Idrus, R. B. H. et al. (2020). Cardioprotective effects of honey and its constituent: An evidence-based review of laboratory studies and clinical trials, International Journal of Environmental Research and Public Health, 17(10), 3613. https://doi.org/10.3390/ijerph17103613.

James, A. and Zikankuba, V. (2017). Moringa oleifera a potential tree for nutrition security in sub-Sahara Africa, American Journal of Research Communication, 5(4), 1-14

Jo, O. et al. (2012). Effects of ethanolic extract of Moringa oleifera laves on lead acetate induced liver damage in adult wistar, International Journal of Biotechnology and Biomedical Research. 2.

Jo, O. (2012) Healing and Prophylactic Effects of Moringa oleifera Leaf Extract on Lead Induced Damage to Haematological and Bone Marrow Elements in Adult Wistar Rat Model. Journal of Aquaculture Research & Development, 01(08), 1–5. https://doi.org/10.4172/scientificreports.386.

Mahmoud, U., Ebied, A.- and Mohamed, S. (2013). Effect of lead on some haematological and biochemical characteristics of Clarias gariepinus dietary supplemented with lycopene and vitamin E. Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, 5(1), 67-89 https://doi.org/10.21608/eajbsc.2013.16112.

Maina, I. W., Obuseng, V. and Nareetsile, F. (2016) ‘Use of Moringa oleifera (Moringa) Seed Pods and Sclerocarya birrea (Morula) Nut Shells fo1. Maina, I. W., Obuseng, V. & Nareetsile, F. Use of Moringa oleifera (Moringa) Seed Pods and Sclerocarya birrea (Morula) Nut Shells for Removal of Heavy Metals from ’, Journal of Chemistry. https://doi.org/10.1155/2016/9312952.

Meyer, P. A. et al. (2003). Surveillance for elevated blood lead levels among children--United States, 1997-2001. MMWR. Surveillance summaries : Morbidity and mortality weekly report. Surveillance summaries/CDC, 5(10), 1-21.

Misra, H. P., & Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. The Journal of biological chemistry, 247(10), 3170–3175.

Ochei, J. and Kolhatkar, A. (2008). Medical Laboratory Science, Theory and Practices. Tata McGraw-Hill.

Okareh, O. and Adeolu, A. (2015) Removal of lead ion from industrial effluent using plantain (Musa paradisiaca) wastes. British Journal of Applied Science & Technology, 8(3), 267–276 https://doi.org/10.9734/bjast/2015/16989.

Othman, N. (2012) Does honey have the characteristics of natural cancer vaccine?, Journal of Traditional and Complementary Medicine, 2(4), 276–283, https://doi.org/10.1016/S2225-4110(16)30113-4.

Rauch, E., Linder, C. and Dallasega, P. (2020). Anthropocentric perspective of production before and within Industry 4.0. Computers and Industrial Engineering, 139, 105-644, https://doi.org/10.1016/j.cie.2019.01.018.

Reddy, D. H. K. et al. (2011). Biosorption of Ni(II) from aqueous phase by Moringa oleifera bark, a low cost biosorbent. Desalination, 268, 150-157 https://doi.org/10.1016/j.desal.2010.10.011.

Reddy, D. H. K. et al. (2012). Optimization of Cd(II), Cu(II) and Ni(II) biosorption by chemically modified Moringa oleifera leaves powder. Carbohydrate Polymers, 88, 1077-1086, https://doi.org/10.1016/j.carbpol.2012.01.073.

Sharayu, R. and Asmita, M. (2017). Beneficial effect of Moringa oleifera on Lead induced Oxidative stress. International J. of Life Sciences, 5(1), 63–72.

Tandon, S. K. and Singh, S. (1994). Protection of lead-induced toxicity by honey in rats. Pharmaceutical Biology, 32(2), 149–153. https://doi.org/10.3109/13880209409082986.

Tietz, N.W., Burtis, C.A., Duncan, P., Ervin, K., Petitclerc, C.J., Rinker, A.D., Shuey, D. and Zygowicz, E.R. (1983) A Reference Method for Measurement of Alkaline Phosphatase Activity in Human Serum. Clinical Chemistry, 29, 751-761.

Tort, L., Torres, P., Hidalgo, J., 1988. The effects of sublethal concentrations of cadmium on haematological parameters in the dogfish Scyliorhinus canicula. J. Fish Biol. 32, 277–282.

Varshney, R., & Kale, R. K. (1990). Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. International journal of radiation biology, 58(5), 733–743. https://doi.org/10.1080/09553009014552121

Waykar, B. B. and Alqadhi, Y. A. (2018) ‘Administration of honey and royal jelly ameliorate cisplatin induced changes in liver and kidney function in rat’, Biomedical and Pharmacology Journal, 11(4), pp. 2191–2199. https://doi.org/10.13005/bpj/1601.

Yadav, G. and Desai, T. N. (2016). Lean Six Sigma: a categorized review of the literature, International Journal of Lean Six Sigma, 7(1), 2–24. https://doi.org/10.1108/IJLSS-05-2015-0015.



DOI: https://doi.org/10.22146/ifnp.77925

Article Metrics

Abstract views : 835 | views : 572

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

Journal of Indonesian Food and Nutrition Progress have been indexed by: 

   

 

This works is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.