Modification of Cassava Starch With Combination of Steaming and Acid Hydrolysis and Use as Encapsulant in Nanoencapsulation of Cocoa Leaf Crude Extract (Theobroma cacao L)

Ratih Kumala Dewi(1*), Supriyanto Supiyanto(2), Yudi Pranoto(3), Agnes Murdiati(4)

(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(4) Universitas Gadjah Mada
(*) Corresponding Author


The utilization of native cassava starch in the food industry is limited. It needs a modified process to increase its utilization. This study aimed to evaluate the effect of a combination of steaming and acid hydrolysis and determine the best temperature and hydrolysis time that is suitable to apply as an encapsulant in the nanoencapsulation process of cocoa leaf crude extract. Modification of cassava starch used 50 ℃, 60 ℃, and 70 ℃ temperatures for 30, 60, and 90 minutes with HCl pH 1. The result showed that modified cassava starch produced from a combination of steaming and acid hydrolysis at 70℃ for 30 minutes has the best specifications with 60.48% solubility, 12.38% hygroscopicity, pasting profile (PV=48; BV=3; FV=66; SV=21). Then, it is combined with Arabic gum and used in the nanoencapsulation process using spray drying. This study showed that encapsulation can protect phenolic compounds of cocoa leaf crude extract resulting brownish-red color surrounded by a black circle. Nanocapsule powder has 13.56% moisture content, 350.3 nm particle size, 16.93 zeta potential, and 84.30% encapsulation efficiency. The combination of steaming and acid hydrolysis at 70 ℃ for 30 minutes produces modified cassava starch which is suitable for use as an encapsulant in the nanoencapsulation process of crude extract of cocoa leaves.


acid hydrolysis; cocoa leaf crude extract; modified starch; nanoencapsulation; steaming

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Akdinez, B., Sumnu and Sahin, G.S. 2017. The Effects of Maltodextrin and Gum Arabic on Encapsulation of Onion Skin Phenolic Compounds. Chemical Engineering Transactions 57.

Anonim. 2005. Official Methods of Analysis of the Association of Official Analytical Chemists. Chemist Inc. New York

Ali, D.Y, Darmadji, P., dan Pranoto, Y. 2014. Optimasi Nanoenkapsulasi Asap Cair Tempurung Kelapa Dengan Response Surface Methodology dan Karakterisasi Nanokapsul. Jurnal Teknologi dan Industri Pangan, 1(25).

Aruba, K.L., Lope, G.T., Shahab, S. and Dumonceaux, T. 2014 Pretreatment and fractionation of barley straw using Steam Explosion at low severity factor. Biomass and Bioenergy 66: 286-300.

Babu, A.S., Parimalavalli, R., and Jagannadham, A. 2014. Chemical and structural properties of sweet potato starch treated with an organic and inorganic acid. J Food Sci Technol 10 (1).

Budijanto, S., dan Yuliyanti. 2012. Studi persiapan tepung sorgum (Sorghum bicolor L. Moench) dan aplikasinya pada pembuaan beras analog. Jurnal Teknologi Pertanian, 13 (3): 177-186.

Caliskan, G dan Dirim, S.N. 2016. The effect of different drying processes and the amounts of maltodextrin addition on the powder properties of sumac extract powders. Powder Technology, 287:308–314

Carvajal, M., Diaz, B., Tores, L.S., Perez, J.J., Beltran, L.A., Aparicio, A.J. and Lopez, G.G. 2010. Nanoencapsulation: A New Trend in Food Engineering Processing. Food Eng Rev 2:39–50.

Chen, J., dan Jane, J. (1994) Preparation of Granular Cold Water-Soluble Starches by Alcoholic-Alkaline Treatment, Carbohydrates, 71(6), 618–622.

Cvitanovic, A. B., S. Radoslava., Verica., K., Drazenka., Iva, J., Viktor, N., dan Branko, B. 2011. Encapsulation of Polyphenolic Antioxidants from Medicinal Plant Extracts in Alginate Chitosan System Enhanced with Ascorbic Acid by Electrostatic Extrusion. Food Research International, (44): 1094-1101.

Febriyanti, I dan Setyowati, A. 2014. Sifat fisik instan temulawak (curcuma xanthorhia Roxb) dengan berbagai rasio penambahan gum arab dan maltodekstrin dari ekstrak hasil maserasi. Jurnal Agribisnis, 5(1):42-57.

Gharsallaoui, A., Roudaut, G., Chambin, O., Voilley, A., and Saurel, R., 2007. Applications of spray-drying in microencapsulation of food ingredients: An overview. Food Research International, 40: 1107–112.

Harris, R., Lecumberi, E., Aparicio, I.M., Mengibar, M. and Heras, A. 2010. Chitosan nanoparticles and microsphere for the encapsulation of natural antioxidants extracted from Ilex paraguariensis. Carbohyd Polym 84: 803-806.

Khazaei, K.M., Jafari, S.M., Ghorbani, M., dan Kakhki, A.H. 2014. The Effects of Maltodextrin and Gum Arabic on Encapsulation of Onion Skin Phenolic Compounds. Carbohydrate Polymers 105:57–62.

Liu, Y., Yan, S., Yan, L., Shaochun, X., Jiangsu, T., Juntao, D. and Yao, XX 2011 Preparation and Characterization of α-galactosidase- Loaded Chitosan Nanoparticles for Use in Foods. Carbohydrate Polymers 83: 1162-1168.

Mucha, J.W., Hasani, M. and Theliander, H. 2017 Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction. Bioresource Technology 241:120-126.

Osman, H., Nasarudin, R., and Lee, S.R. 2004 Extracts of cocoa (Theobroma cacao L) leave and their antioxidation potential, Food Chemistry 86 41-46.

Phisut, N., 2012. Spray drying Technique of Fruit Juice Powder: Some Factors Influencing The Properties of Product. Mini Review. International Food Research Journal, 19 (4): 1297–1306

Robert, P. dan Fredes, C. 2015. The Encapsulation of Anthocyanins from Berry-Type Fruits. Trends in Foods. Molecules, 20:5875-5888

Saloko, S., Darmadji, P., Setiaji, B. and Pranoto, Y. 2012. Structural analysis of spray-dried coconut shell liquid smoke powder. J Teknol dan Industri Pangan 23:173-179.

Singh, J., Kaur, L., and McCarthy, O.J. 2007 Factors influencing the physicochemical, morphological, thermal and rheological properties of some chemically modified starches for food application review. Food Hydrocolloids 21(1):1-22.

Soloway, S. and Wilen, S.H. 1952 Improved Ferric Chloride Test for Phenols. Analytical Chemistry 24 (6): 979–983.

Sun, J., Zhao, R., Zeng, J., Li, G., and Li, X. 2010. Characterization of Dextrins with Different Dextrose Equivalents. Molecules, 15:5162-5173.

Utami, K. 2015. Optimasi Rasio Gum Arab dan Maltodekstrin Sebagai Enkapsulan dan Kondisi Homogenisasi Pada Proses Nanoenkapsulasi Asap Cair Tempurung Kelapa. Tesis. Teknologi Hasil Perkebunan. Universitas Gadjah Mada.

Wang, Y, and Wang, L. 2010. Structures and Properties of Commercial Maltodextrins from Corn, Potato, and Rice Starches. Starch, 52 (8-9): 296-304.

Xing, J.J., Liu Y., Li, D., Wang and Adhikari, J.B. 2017 Heat-moisture treatment and acid hydrolysis of corn starch in different sequences. Food Science and Technology 79:11-20.

Yadav, B.S., Guleria, P., and Yadav, R.B. 2013 Hydrothermal modification of Indian water Chesnut starch: Influence of heat-moisture treatment and annealing on the physicochemical, gelatinization, and pasting characteristic. Food Science and Technology 53: 211-217.

Yang, X.R., Yuan, Y.W., Kai, K.L., Jing, L., Cheng, C.L., Xiang, G.S., Chun, H.K, Ping, C.L., Chuang, X.Y. and Sing, XH 2011 Cocoa tea (Camellia ptilophylla Chang), a natural decaffeinated species of tea – Recommendations on the proper way of preparation for consumption. Journal of Functional Foods 3: 305-312.

Zheng, M.Q., Jin, Z.Y. and Zhang, Z.P. 2007. Effect of Cross-Linking and Esterification on Hygroscopicity and Surface Activity of Cassava Maltodextrins. Food Chemistry 103:1375-1379.


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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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