Detection of Fish Freshness Using Immobilized ADP-ase and 5'-Nucleotidase on Polyacrylamide Gel
https://doi.org/10.22146/jifnp.54
Djagal W Marseno
(1*), Retno Indrati
(2), Sudarmanto Sudarmanto
(3)
(1) Gadjah Mada University
(2) Gadjah Mada University
(3) Gadjah Mada University
(*) Corresponding Author
Abstract
The presence of ADP and IMP in the muscle of fish could be used as an indicator of its freshness. These metabolites could be detected enzymaticaly using ADPase and 51-nucleotidase. The aim of this research was to determine the presence of ADP and IMP in the muscle of fish qualitatively, using immobilized ADPase and 5'-nucleotidase on polyacrylamide gel. The results showed that in the non-immobilized form, ADPase has an optimum pH of 6 and stable at pH 5.5-10, while 5'-nucleotidase has two optima pH of 6.5 and 9 and it was stable at pH 7-10. Optimum temperature of ADPase and 5'-nucleotidase was 45 and 50°C, respectively. In the immobilized form, the activity of ADPase was optimum at pH 6 and it was still stable at pH 5.5 - 7.0 after storage at -20°Cfor 90 days, while 5'-nucleotidase was still stable at pH Z5-10 after storage at -20°C for 90 days. Both enzymes were more stable in frozen storage than that of chilled storage. Sensitivity of both enzymes to detect the fish freshness during storage was affected by the presence of free inorganic phosphate derived from other phosphate-containing metabolites.
![](/public/site/images/admin/icons/icon-doi.png)
DOI:
https://doi.org/10.22146/jifnp.54
Article Metrics
![](/public/site/images/admin/icons/icon-graph.png)
Abstract views : 1044
|
![](/public/site/images/admin/icons/icon-pdf.png)
views : 478
Refbacks
- There are currently no refbacks.
![Creative Commons License](http://i.creativecommons.org/l/by-sa/4.0/88x31.png)
This work is licensed under a
Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.
Journal of Indonesian Food and Nutrition Progress have been indexed by:
![](/public/site/images/anisaquentina99/sinta_logo_resize1.png)
This works is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
![](/public/site/images/anisaquentina99/CC_BY-SA_icon.svg_resize_1.png)