Association of Quantitative Characteristics with Growth Hormone Gene (GH Gene) in Kerinci Duck Using PCR-RFLP Method
Amalia Fildzah Ghassani(1), Depison Depison(2*), Helmi Ediyanto(3)
(1) Animal Science Study Program, Faculty of Animal Science, University of Jambi, Jambi, 36361
(2) Animal Science Study Program, Faculty of Animal Science, University of Jambi, Jambi, 36361
(3) Animal Science Study Program, Faculty of Animal Science, University of Jambi, Jambi, 36361
(*) Corresponding Author
Abstract
This study aims to determine the association of quantitative characteristics with growth hormone gene (GH gene) in kerinci duck using PCR-RFLP method. Samples total used was 96 Kerinci ducks consisting of 43 males and 53 females and 96 blood samples. DNA was extracted using the protocol Genomic DNA Purification Kit from Promega and then amplified by PCR using a pair of primers5'-CAA GGA ACA GAG GGT TTC CA-3' and Revers : 3'-GGG AGA TAG GGC AAA CAT CA-5', with a length of product 855 bp. The amplification product was cut using restriction enzyme AluI with the AG↓CT cutting site. Growth hormone/AluI fragments of Kerinci duck were electrophoresed using 1.5% agarose gel and visualized using doc gel. Data collected includes body weight, weight gain, body measurements, and blood of Kerinci ducks. The differences in body weight, body weight gain, and body measurements, as well as differences in body weight between genotypes were tested by T-test. The determinants of the size and shape of Kerinci duck were analyzed using PCA. This study showed that the body weight, weight gain, and body sizes of male Kerinci ducks were significantly different (P<0.05) than female Kerinci ducks. The Kerinci duck GH|AluI gene is polymorphic with three genotypes, i.e, +/+ of (49%), +/- of (39.6%), and -/- of (11.5%), and two alleles, namely (+) by 69% and (–) by 31%. Conclusion: body weight, weight gain, and body sizes of male Kerinci ducks were higher than female. The size identifier of male and female Kerinci ducks were sternum, shank lengh, and shank circumference, while shape identifier was wing length. GH Genes|AluI in Kerinci duck is polymorphic. GH gene |AluI of Kerinci ducks was associated with body weight, weight gain, and body measurements, and the best is the genotype (+/+).
Keywords
Full Text:
PDFReferences
Allendorf F, W. G. Luikart, and S. N. Aitken. 2013. Conservation and the genetics of populations. Blackwell Publishing. Oxford, UK.
Assefa H. and A. Melesse. 2018. Morphological and morphometric characterization of indigenous chicken populations in Sheka Zone. South Western Ethiopia. Poult. Fish. Wildl. Sci. 16: 1-9.
Asiamah, C. Y. Xue, L. L. Lu, K. Zou, Z. Zhao, and Y. Su. 2020. Evaluation of growth performance on family breeding of the Leizhou Black Duck: A preliminary study. Vet. Med. Sci. 6: 500-510.
Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32: 314–331.
Brahmantiyo, B., L. H., Prasetyo, A. R., Setioko, and R. H. Mulyono. 2003. Estimation of genetic distance and differentiating variables of duck lines (Alabio, Bali, Khaki Campbell, Mojosari and Pegagan) through morphometric analysis. JITV. 8:1-7.
Daud, M., M. A. Yaman, and A. Syahrien. 2019. The use of Leubiem fish waste (chanthidermis maculatus) in rations on the performance of laying ducks. In Proceedings of the USM National Seminar, 2: 410-419.
El-Deghadi, A. S., W. A. H. Ali, and M. G. Gharib. 2022. Study for some body weight and egg traits in Domyati and Khaki-Campbell Ducks. Open Journal of Agricultural Research, 2: 29-36.
Fachtiyah, E. L. A. Rumingtyas, S. Widyarti, and S. Rahayu. 2011. Molecular Biology. Erlangga, Jakarta.
Fatmarischa, N., S. Sutopo, and S. Johari. 2013. Goat body size in three districts of Central Java province. Animal Science: Journal of Animal Science Research 11: 106-112.
Gaspersz, V. 2006. Analysis Techniques in Experimental Research. Tarsito Publisher, Bandung.
Gultom, L. H. M., Gushairiyanto, and Depison. 2021. Correlation of Sentul chicken body weight at Doc age of 1, 2 and 3 months. Indonesian Journal of Animal Science 16: 273-276.
Hasan, N., S. Choudhary, N. Naaz, N. Sharma, and R. A. Laskar. 2021. Recen advancements in molecular marker assisted selection and applications in plant breeding programmes. J. Gen. Engin. Biotech. 19:1 26.
Hidayati, E. Saleh, and T. Aulawi. 2016. Identification of the genetic diversity of bmpr-1b (bone morphogenetic protein receptor IB) in Arabic chicken, Kampung chicken, and laying hens using PCR-RFLP. Anim. J. 13: 1–12.
Hikmawaty, A. Gunawan, R. R. Noor, and Jakaria. 2014. Identification of body size and body shape of bali cattle in several breeding centers through Principal Component Analysis approach. J. Anim. Prod. Sci. Tech. 2: 231-237.
Hosnedlova, B., K. Vernerova, R. Kizek, R. Bozzi, J. Kadlec, V. Curn, F. Kouba, C. Fernandez, V. Machander, and H. Horna. 2020. Associations between IGF1, IGFBP2 and TGFß3genes polymorphisms and growth performance of broiler chicken lines. Animals 10: 1-24.
Kamagi, D. D. 2017. Isolation and mtdna amplification of red jungle fowl and native chicken (Gallus gallus) of North Sulawesi. Journal of Science, Mathematics and Education 5: 162-167.
Kaps, M. and W. R. Lamberson. 2004. Biostatistics for Animal Science. CABI.
Kazemi, M. L. E. McBreairty, D. R. Chizen, R. A. Pierson, P. D. Chilibeck, and G. A. Zello. 2018. A comparison of a pulse-based diet and the therapeutic lifestyle changes diet in combination with exercise and health counselling on the cardio-metabolic risk profile in women with polycystic ovary syndrome: a randomized controlled trial. Nutrients 10:1-30
Mahmudi, M., R. Priyanto, and J. Jakaria. 2019. The morphometric characteristics of Aceh cattle, PO cattle and Bali cattle were based on principal component analysis (AKU). J. Anim. Prod. Sci. Tech. 7: 35-40.
Mardiah, T., Depison, and E. Adiyanto. 2021. Phenotype Diversity and Gene Myostatin (MSTN) of Bangkok Chicken using PCR-RFLP. Buletin Peternakan 45: 233-240.
Mazurowski, A., A. Frieske, D. Kokoszyński, S. Mroczkowski, Z. Bernacki, and A. Wilkanowska. 2015. Examination of growth hormone (GH) gene polymorphism and its association with body weight and selected body dimensions in ducks. Folia Biologica (Kraków) 63: 43-50.
Mulliadi, D. and J. Arifin. 2010. Estimation of population equilibrium and heterozygosity using blood albumin protein pattern in a thin tailed sheep population in the Indramayu Region (Prediction equilibrium of population used blood albumin pattern of thin tailed sheep pop. Journal of Animal Science Universitas Padjadjaran 10: 65-72.
Muzani, A., B., C. Brahmantiyo, Sumantri, and A. Tapyadi. 2005. Estimation of genetic distance in Cihateup, Cirebon and Mojosari ducks. Livestock Media 28:109-116.
Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.
Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York.
Nova, T. and Yurnalis. 2016. Genetic diversity of growth hormone gene (GH|MboI) in Sikumbang ducks using PCR-RFLP as a marker. Indonesian Animal Husbandry Journal 18: 44-52.
Pagala, M. A., A. Selamet, B. Rusli, and H. Has. 2018. Phenotypic and genotypic characteristics of the GH (growth hormone) gene in Tolaki chickens. Journal of Tropical Animal Husbandry Science and Technology 5: 1-4.
Pagala, M. A., A. S. Badaruddin, and R. Has. 2017. Phenotypic and genotypic characteristics of the GH (growth hormone) gene in Tolaki chickens. J. Trop. Anim. Sci. Tech. 5: 1–4.
Puja, I. K., I. N., Wandia, P. Suastika, and I. N. Sulabda. 2013. Asosiasi polimorfisme genetika lokus deoxynucleic acid (dna) mikrosatelit gen bovine lymphocyte antigen (BoLA) dengan kualitas semen pada sapi Bali. Jurnal Kedokteran Hewan-Indonesian Journal of Veterinary Sciences 7: 163-165.
Puteri, I. K., Gushairiyanto, and Depison. 2020. Growth patterns, body weight, and morphometric of KUB chicken, Sentul chicken and Arab chicken. Buletin Peternakan 44: 67-72.
Putra, A., Rukmiasih, and R. Afnan. 2015. Percentage and carcass quality of Cihateup-Alabio (CA) ducks at different slaughter ages. Department of Animal Production Science and Technology 3: 27-32.
Rahayu, F. F., Depison, and Gushairiyanto. 2021. Performance of kampung Super chicken and Bangkok chicken first generation (G1) until the age of 12 weeks. Livestock and Animal Research 19: 326-336.
Sari, M., Depison., Gushariyanto, and E. Wiyanto. 2021. The relationship between egg weight and hatching weight and hatching weight with body weight of Merawang g1 chickens until the age of 4 months. Animal Journal 18: 147-159.
Setiyono, E. and R. P. Bekti. 2019. Morphological characteristics and testicular development of alabio ducks (Anas platyrhynchos Borneo) grower period. Life Sci. 8: 170-180.
Sitanggang, E. N, Hasnudi, and Hamdan. 2016. The diversity of qualitative and morphometric traits between Kampung chickens, Bangkok chickens, Katai chickens, Burmese chickens, Bagon chickens and Magon chickens in Medan. Journal of Integrative Animal Husbandry 3: 167–189.
Soeparno. 2005. Meat Science and Technology. 4 th printing. Gadjah Mada University Press, Yogyakarta.
Suryana, R. R., P. S., L. H., Prasetyo, Hardjosworo, and M. Yasin. 2014. Morphological characterization of the alabio duck (Anas platyrhynchos Borneo) in the development center area of South Kalimantan. In Proceedings of the National Seminar “Innovation of Location-Specific Agricultural Technology”, Banjarbaru, 6-7.
Syaifudin, Rukmiasih, and R. Afnan. 2015. Performance of male and female Alabio ducks based on hatching weight grouping. J. Prod. Sci. Tech. Livest. Products 3: 83-88.
Tarigan, J. H. 2015. Identification of body weight and body measurements of Balinese ducks (the case in the herd of Bead Sari ducks in Lepang Hamlet, Takmung Village, Banjarangkan District, Klungkung Regency, Bali Province). Studens E-Jurnal 4: 1-7.
Wahyuni, Depison, and Gushairiyanto. 2022. Comparison of productivity of sentul and kampung chickens until the age of 3 months in the first generation selection population (G1). Bulletin of Animal Science 46: 23-30.
Wang, J. L. Raskin, D. C. Samuels, Y. Shyr, and Y. Guo. 2015. Genome measures used for quality control are dependent on gene function and ancestry. Bioinformatics 31: 318–323.
Yang, Y. T. Guo, J. Luo, R. Zhang, J. Zhao, M. L. Warburton, Y. Xiao, and J. Yan. 2022. Target-oriented prioritization: targeted selection strategy by integrating organismal and molecular traits through predictive analytics in breeding. Genome Biology: 1-19.
Yurnalis, Arnim, D. E. Putra, Z. Kamsa, and T. Afriani. 2019. Identification of gh gene polymorphisms and their association with body weight in Bayang Duck, Local Duck from West Sumatra, Indonesia. International Conference on Sustainable Agriculture, Food and Energy: 1-7.
DOI: https://doi.org/10.21059/buletinpeternak.v46i4.75810
Article Metrics
Abstract views : 1298 | views : 727Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Buletin Peternakan (Bulletin of Animal Science) Indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.