* Corresponding Author
Phylogenetic Study of Madura Cattle Based on Mitochondrial Cyt b and D-loop Sequences
https://doi.org/10.21059/buletinpeternak.v45i1.48557 Nirmala Fitria Firdhausi(1*), Achmad Farajallah(2), Dyah Perwitasari(3)
(1) Department of Biology, Faculty of Sains and Technology, Universitas Islam Negeri Surabaya, Surabaya, 60237, Indonesia
(2) Department of Biology, Faculty of Math and Science, Institut Pertanian Bogor, Bogor, 16680, Indonesia
(3) Department of Biology, Faculty of Math and Science, Institut Pertanian Bogor, Bogor, 16680, Indonesia
(*) Corresponding Author
Abstract
Madura Cattle is one breed of local cattle from Indonesia. Madura cattle are estimated to originate from a crossbreeding between Bos indicus and Bos javanicus. Another presumption is that Madura cattle are the result of a crossbreeding between B. indicus males and mixed B. javanicus or Bos taurus. Tracing the history of Madura cross and another cattle phylogenetic based on maternal lineage can be done by analyzing the variation of the mitochondrial genome (mtDNA). The purpose of this study was to determine the clarity of the origin of Madura cattle based on maternal lineage using mtDNA markers Cyt b and D-loop. This research is expected to provide genetic information and the origin of Madura cattle, so that it can be used to help improve the breeding and conservation program for Madura cattle. The results of the phylogeny tree reconstruction, using the Cyt b and D-loop genes showed that Madura cattle originated from Sampang region (Polagan, Golbung, and Komis) were grouped into two types of maternal origin. Madura cattle clade I are grouped with B. indicus and B. taurus, while Madura cattle clade II are grouped with B. javanicus. A crossbreeding between B. javanicus and B. indicus is estimated to have been carried out since the entry of Hindu culture brought by the India peoples to Indonesia around 1800 years ago. The crossing between B. javanicus and B. indicus was then more intensively carried out at the time of the government's promoting the development of Ongol cattles (B. indicus) in the days of the Dutch East Indies. The length segment of Cyt b that can be amplified is 230 bp and the D-loop segment of varying length, 577 bp for the Madura 41 and 29 samples, and 624 bp for sample 32.
Keywords
Full Text:
PDFReferences
Avise, J. C. 1994. Molecular Markers, Natural History and Evolution. Chapman and Hall, New York.
Dakay, I., D. Marton, S. Bene, B. Kiss, Z. Zsuppan, and F. Szabo. 2006. The Age at first calving and the longevity of beef cows in Hungary. Arch. Tierz. Dummertorf. 49: 417-425.
Dwiyanto. 2008. Pemanfaatan sumberdaya local dan inovasi tekhnologi dalam mendukung pengembangan sapi potong di Indonesia. Pengembangan Informasi Pertanian 1: 173-178.
Farajallah, A. 2005. Kajian pembentukan ruas berulang dalam daerah pengontrol genom mitokondria labi-labi [Laporan Penelitian]. Lembaga Penelitian dan Pemberdayaan Masyarakat, Institut Pertanian Bogor, Bogor.
Gemmel, N. J., P. S. Western, J. A. M Watson, and Graves. 1996. Evolution of the mammalian mitochondrial control region comparisons of control region sequences between monotreme and therian mammals. Mol. Biol. Evol. 13: 798-808.
Hiendleder, S., B. R. Kaupe, Wassmuth, and A. Janke. 2002. Molecular analysis of wild and domestic sheep questions current nomenclature and provides evidence for domestication from two different subspecies. Proc. R. Soc. Lond. 269: 893–904.
Kusdiantoro, M., M. Olsson, H. T. A. Tol, S. Mikko, B. H. Vlamings, G. Andersson, H. R. Martinez, B. Purwantara, Paling, B. Colender, and J. A. Lenstra. 2009. The Origin of Indonesian Cattle. PloS ONE 4: 1-5.
Maksum, K. 1993. Hasil-hasil penelitian sapi Madura di Sub Balai Penelitian Ternak Grati-Pasuruan. Di dalam: Hasil Penelitian dan Pengembangan Sapi Madura. Prosiding Pertemuan Ilmiah, Sumenep, 11-12 Oktober 1992. Sumenep.
Mannen, H., S. Tsuji, R. T. Loftus, and D. G. Bradley. 1998. Mitochondrial DNA variation and evolution of Japanese black cattle (Bos Taurus). Genetic 150: 1169-1175.
McHugh, D. E. 1997. Molecular biogeography and genetic structure of domestic cattle (Thesis). University of Dublin.
Namikawa, T. 1981. Geographic Distribution of Bovine hemoglobin-beta (hbb) alleles and phylogenetic analysis of the cattle in Eastern Asia. Zeitschift fur Tierzuchtung and Zuchtungsbiologie 98: 151-159.
Nijman, I. J., M. Otsen, E. L. C. Verkaar, C. D. Ruitjer, E. Hanekamp, J. W. Ochieng, S. Shamshad, J. E. O. Rege, O. Hannote, M. W. Barwegen, T. Sulawati, and J. A. Lenstra. 2003. Hibridization of Banteng (Bosjavanicus) and Zebu (Bosindicus) revealed by Mithocondrial DNA, satellite DNA, AFLP microsatellites. Heredity 90: 10-16.
Pessole, G., C. Gissi, A. D. Chirico, and C. Saccone. 1998. Nucleotide substitution rate of mammalian mitochondrial genomes. J. Mol. Evol. 48: 427-434.
Payne, W. J. A. and J. Hodges. 1997. Tropical Cattle: Origins, Breed, and Breeding Policies. Oxford: Blackwell Science Ltd.
Rollinson, D. H. L. 1984. Bali Cattle. In: Evolution of Domesticated Animals. Mason, i.l. (ed.). New York: Longman
Sambrook, J., E. F. Fritsch, and T. Miniatis. 1989. Molecular Cloning: A laboratory Manual. Ed ke-8. New York: Cold Spring Harbor Laboratory Press.
Surjoatmodjo, M. 1993. Asal usul sapi Madura ditinjau dari hasil pengukuran bagian-bagian tubuhnya. Di Dalam: Hasil penelitian dan pengembangan Sapi Madura. Prosiding Pertemuan Ilmiah, Sumenep, 11-12 Oktober 1992. Sumenep.
Simm, G. 2000. Genetic Improvement of Cattle and Sheep. United Kingdom, Farming Press.
Tamura, K., J. Dudley, M. Nei and S. Kumar. 2007. MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 10.1093/molbev/msm092.
Tapio, M. and I. Grigaliunaite. 2002. Is there a role for mitochondrial in sheep breeding? VeterinarijaIr Zootechnika 18: 108-111.
Tegelstrom, 1986. Mitochondrial DNA in natural population: an improved routine for screening of genetic variation based on sensitive silver staining. Electrophoresis 7: 226-229.
Uggla, C. M. 2008. Investigating genetic variability within specific indigenous Indonesia cattle breed. Disertasi. Swedish University of Agricultural Science.
Vietmeyer, N. D. 1983. Little Known Asian Animals With a Promising Economic Future. National Academy Press, Washington.
Williamson, G. and W. J. A. Payne. 1965. An Introduction to Animal Husbandry In The Trophic. Longman Group Limited, London.
DOI: https://doi.org/10.21059/buletinpeternak.v45i1.48557
Article Metrics
Abstract views : 1994
| 
views : 2227
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Buletin Peternakan (Bulletin of Animal Science) Indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.