Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

Widya Pintaka Bayu Putra; Saiful Anwar; Syahruddin Said; Romanos Albert Adhitya Indarto; Putri Wulandari

doi:10.21059/buletinpeternak.v43i1.38227

Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

https://doi.org/10.21059/buletinpeternak.v43i1.38227

Widya Pintaka Bayu Putra^(1*), Saiful Anwar⁽²⁾, Syahruddin Said⁽³⁾, Romanos Albert Adhitya Indarto⁽⁴⁾, Putri Wulandari⁽⁵⁾

(1) Research Center for Biotechnology - Indonesian Institute of Sciences, West Java, 16911, Indonesia
(2) Research Center for Biotechnology - Indonesian Institute of Sciences, West Java, 16911, Indonesia
(3) Research Center for Biotechnology - Indonesian Institute of Sciences, West Java, 16911, Indonesia
(4) Department of Biology, Faculty of Life Science, Surya University, Banten, 15810, Indonesia
(5) Department of Biology, Faculty of Science and Technology, Jambi University, Jambi, 36361, Indonesia
(*) Corresponding Author

Abstract

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (n_e) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

Keywords

Leptin; Pasundan cattle; PCR-RFLP; Thyroglobulin

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References

Almeida, S. E. M., E. A. Almeida, J. F. C. Moraes, and T. A. Weimer. 2003. Molecular markers in LEP gene and reproductive performance of beef cattle. J. Anim. Breed. Genet. 120: 106-113.

Anonymous. 2014. Penetapan rumpun sapi Pasundan. http://www. http://pustaka.ditjenpkh.pertanian.go.id/berita/komoditas/sapi%20pasundan.pdf. Accessed 15 August 2018.

Anton, I., K. Katalin, H. Gabriella, Valeria, S. Ference, E. Istvant, R. Jozsef, Z. Attila and B. Klaus-Peter. 2012. Effect of DGAT1, leptin and TG gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary. Arch. Tierz. 55: 307-314.

Anwar, S., C. P. Aditya, A. S. Wulandari, P. P. Agung, W. P. B. Putra, and S. Said. 2016. Genetic polymorphism analysis of 5'untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different region of Indonesia. Indonesian J. Trop. Anim. Agric. 42: 175-184.

Arifin, J., S. B. Komar, E. Y. Setyowati, U. Yunasaf, A. Anang, Indrijani, and Sulasmi. 2015. Sebaran gen, keseimbangan populasi dan ukuran populasi efektif sapi Pasundan pasca migrasi di Majalengka. Jurnal Ilmu Ternak. 15: 1-7.

Baas, F., G. J. van Ommen, H. Bikker, A. C. Arnberg, and J. J. de Vijlder. 1986. The human thyroglobulin gene is over 300 kb long and contains introns of up to 64 kb. Nucleic. Acids. Res. 14: 5171-5186.

Barendse, W. J. 1999. Assessing lipid metabolism. International Patent Application WO9923248 US6383751 (PCT/AU98/00882). http://www.ep.espacenet.com. Accessed 15 August 2018.

Barendse, W. J., R. J. Bunch, M. Thomas, S. Armitage, S. Baud, and N. Donaldson. 2004. The TG5 thyroglobulin gene test for a marbling quantitative trait loci evaluated in feedlot cattle. Aust. J. Exp. Agric. 44: 669-674.

Berezovsky, O. V. 2014. Evaluation of gene polymorphism thyroglobulin in different breeds of cattle for dairy and meat cattle performance. http://www.journals.nubip.edu.ua/index.php/Teknologiya/article/viewFile/1966/1915. Accessed 15 August 2018.

Bonilla, C. A., M. S. Rubio, A. M. Sifuentes, G. M. Parra-Bracamonte, V. W. Arellano, M. R. D. Mendez, J. M. Berruecos, R. Ortiz. 2010. Association of CAPN1 316, CAPN1 4751 and TG5 markers with bovine meat quality traits in Mexico. Genet. Mol. Res. 9: 2395-2405.

Carvalho, T. D., F. Siqueira, R. A. A. T. Junior, S. R. Medeiros, G. L. D. Feijo, M. D. S. Junior, I. M. Z. Bleca, and C. O. Soares. 2012. Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle. R. Bras. Zootec. 41: 2162-2168.

Casas, E., S. N. White, D. G. Riley, T. P. Smith, R. A. Brenneman, T. A. Olson, D. D. Johnson, S. W. Coleman, G. L. Bennett, and J. J. Jr Chase. 2005. Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. J. Anim. Sci. 83: 13-19.

Chesnokov, Y. V. and A. M. Artemyeva. 2015. Evaluation of the measure of polymorphism information of genetic diversity. Agric. Biol. 50: 571-578.

Darimont, C., D. Gaillard, A. Gerard, and N. Raymond. 1993. Terminal differentiation of mouse preadipocyte cells: adipogenic and antimitogenic role of triiodothyronine. Mol. Cell. Endocrinol. 98: 67-73.

Falconer, D. S. and T. F. C. Mackay. 1996. Introduction of Quantitative Genetics. 4^thed. Department of Genetics. North Canada State University, Prince George.

Ferchichi, M. A., J. Bayrem, A. Sihem, B. G. Abderrahmane, and R. Boulbaba. 2018. Effect of leptin genetic polymorphism on lameness prevalence in Tunisian Holstein cows. Arch. Anim. Breed. 61: 305-310.

Fernandez, M. E., E. G. Daniel, J. P. Alberto, P. G. Pilar, B. Andreas, G. Guillermo, and P.L. Juan. 2014. Assessing the association of single nucleotide polymorphisms in thyroglobulin gene with age of puberty in bulls. J. Anim. Sci. Tech. 56: 1-4.

Fortes, M. R. S., R. A. Curi, L. A. L. Chardulo, A. C. Silveira, M. E. O. D. Assumpção, J. A.Visintin, and H. N. de Oliveira, 2009. Bovine gene polymorphisms related to fat deposition and meat tenderness. Genet. Mol. Biol. 32: 75-82.

Frunhbeck, G., S. A. Jebb, and A. M. Prentice. 1998. Leptin: physiol pathophysiol. J. Clin. Physiol. 18: 399-419.

Gan, Q. F., L. P. Zhang, J. Y. Li, G. Y. Hou, H. D. Li, X. Gao, H. Y. Ren, J. B. Chen, and S. Z. Xu. 2008. Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. J. Appl. Genet. 49: 251-255.

Hussain, D. A., H. A. Zainab, and A. A. Tabarek. 2017. Genetic structure analysis of leptin gene / Sau3AI and its relationship with body weight in Iraqi and Holstein-Friesian cows population. IOSR-JPBS. 12: 10-13.

Javanmard, A., M. R. Mohammadabadi, G. E. Zarrigabayi, A. A. Gharahedaghi, M. R. Nassiry, A. Javadmansh, and N. Asadzadeh. 2008. Polymorphism within the intron region of the bovine leptin gene in Iranian Serabi cattle (Iranian Bos indicus). Russ. J. Genet. 44: 495-497.

Khatib, H., I. Zaitoun, Y. M. Chang, C. Maltecca, and P. Boettche. 2007. Evaluation of association between polymorphism within the thyroglobulin gene and milk productiontraits in dairy cattle. J. Anim. Breed. Genet.124:26-28.

Kiyici, J. M., K. Arslan, B. Akyuz, M. Kaliber, E. G. Aksel, and M. U. Cinar. 2018. Relationships between polymorphism of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. Int. J. Dairy Tech. 70: 1-7.

Liefers, S. C., M. F. W. Pas, R. F. Veerkamp, and T. Lende. 2002. Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake and fertility in Holstein heifers. J. Dairy Sci. 85: 1633-1638.

Mappanganro, R., D. P. Rahardja, and H. Sonjaya. 2014. Hubungan antara gen leptin dengan skor kondisi tubuh induk sapi Bali dan persilangannya. Jurnal Sains dan Teknologi. 14: 232-240.

Mears, G. J., P. S. Mir, D. R. C. Bailey, and S. D. M. Jones. 2001. Effect of Wagyu genetics on marbling, backfat and circulating hormones in cattle. Can. J. Anim. Sci. 81: 65-73.

Moore, S. S., C. Li, J. Basarab, W. M. Snelling, J. Kneeland, B. Murdoch, C. Hansen, and B. Benkel. 2003. Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus. J. Anim. Sci. 81: 1919-1925.

Moravcikova, N., A. Trakovicka, H. Eva, B. Jozef, and K. Radovan. 2012. Associations between polymorphisms in the leptin gene and milk production traits in Pinzgau and Slovak Spotted cattle. Act. Agric. Slov. 3: 259-263.

Moussavi, A. H., M. Ahouei, M. R. Nassiry, and A. Javadmanesh. 2006. Association of leptin polymorphism with production, reproduction and plasma glucose level in Iranian Holstein cattle. Asian-Aust. J. Anim. Sci. 19: 627-631.

Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.

Nobari, K., G. Shokoufe, R. N. Mohammdad, T. Mojtaba, and J. Eisa. 2010. Relationship between leptin gene polymorphism with economical traits in Iranian Sistani and Brown Swiss cows. J. Anim. Vet. Adv. 9: 2807-2810.

Oner, Y., Y. Onur, O. Hayrettin, A. Nezih, Y. M. Gulnaz, and K. Abdulkadir. 2017. Associations between GH, PRL, STAT5A, OPN, Pit-1, LEP and FGF2 genes polymorphisms and fertility in Holstein-Friesian heifers. Kafkas. Univ. Vet. Fak. Derg. 23: 527-534

Pannier, L., A. M. Mullen, R. M. Hamill, P. C. Stapleton, and T. Sweeney. 2010. Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat. Sci. 85: 515-518.

Pfister-Genskow, M., H. Hayes, A. Enggen, and M. D. Bishop. 1996. Chromosomal localization of the bovine obesity (OBS) gene. Mamm. Genome. 7: 398-399.

Rasor, C. C., M. G. Thomas, R. M. E. Pas, H. C. Salazar, H. M. Zhang, G. L. Williams, R. L. S. Pas, R. D. Randel, and J. Rios. 2002. Allelic and and genotypic frequencies of the leptin gene Sau3AI - restriction fragment length polymorphism and evaluation of its association with age at puberty in cattle in the southwestern United States and Northern Mexico. The Proff. Anim. Sci. 18: 141-146.

Rincker, C. B., N. A. Pyatt, L. L. Berger, and D. B. Faulkner. 2006. Relationship among GeneSTAR marbling marker, intramuscular fat deposition and expected progeny differences in early weaned Simmental steers. J. Anim. Sci. 84: 686-693.

Said, S., W. P. B. Putra, S. Anwar, P. P. Agung, and H. Yuani. 2017. Phenotypic, morphometric characterization and population structure of Pasundan cattle at West Java, Indonesia. Biodiversitas.18: 1638-1645.

Sedykh, T. A.L. A. Kalashnikova, I. V. Gusev, I. Y. Pavlova, R. S. Gizatullin, and I. Y. Dolmatova. 2016. Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves. Iraqi J. Vet. Sci. 30: 41-48.

Sharifzadeh, A. and A. Doosti. 2012. Investigation of leptin gene polymorphism in Iranian native cattle. Bulgarian J. Vet. Med. 15: 86-92.

Savasci, M. and A. Fatih. 2016. The investigation of calpastatin and thyroglobulin gene polymorphisms in some native cattle breeds. Ankara. Univ. Vet. Fac. Derg. 63: 53-59.

Shin, S. C., and E. R. Chung. 2007. Association of SNP marker in the thyroglobulin gene with carcass and meat quality traits in Korean Cattle. Asian-Aust. J. Anim. Sci. 20: 172-177.

Smas, C. M., and H. S. Sul. 1995. Control of adipocyte differentiation. Biochem. J. 309: 697-710.

Smith, T., M. G. Thomas, T. D. Bidner, J. C. Pascha, and D. E. Franke. 2009. Single nucleotide polymorphisms in Brahman steers and their association with carcass and tenderness traits. Genet. Mol. Res. 8: 39-46.

Trakovicka, A., M. Nina, and K. Radovan. 2013. Genetic polymorphism of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Act. Biochim. Pol. 60: 783-787.

Yardibi, H., E. G. Feraye, A. Atila, A. Iraz, T. H. Gulhan, and O. Kemal. 2013. BTN1A1, FABP3 and TG genes polymorphism in East Anatolian Red cattle breed and South Anatolian cattle breed. Afr. J. Biotechnol. 12: 2802-2807.

Zhang, L. P., Q. F. Gan, G. Y. Hou, H. J. Gao,J. Y. Li, and S. Z. Xu. 2015. Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers. Genet. Mol. Res. 14: 5320-5326.

DOI: https://doi.org/10.21059/buletinpeternak.v43i1.38227