Development and Characterization of Pectin-Based Colon Targeted Pellets Containing Lactobacillus Plantarum FNCC-0461
Keywords:
Lactobacillus plantarum FNCC-0461, colon targeted delivery system, pellets, pectin, extrusion-spheronization
Abstract
Lactobacillus plantarum FNCC-0461 is a lactic acid bacteria isolated from "dadih" a traditional Indonesian food that has potential as a probiotic. Probiotics can show health benefits if they can maintain cell viability of at least 7 log CFU in the distal ileum and colon. However, most probiotics are not resistant to the extreme conditions of the gastrointestinal tract. Probiotic encapsulation in the form of pectin-based colon targeted pellets is a promising delivery system to overcome probiotic viability problems due to the gastrointestinal tract extreme conditions and can assist release to specific target site in colon. Pellets was produced by extrusion-spheronization method using microcrystalline cellulose (MCC), lactose and pectin. Optimization of spheronization process was carried out by varying the spheronization speed and time while the optimization of pellets formula was carried out by varying the concentration of total pectin and the type of coating polymer (cellulose acetate phthalate (CAP) or shellac). The morphology, particle size, moisture content, micromeritic properties, process yield and viability of pellets were evaluated. Release of probiotics on coated and uncoated pellets were examined at pH 1.2, 6.8 and 7.4 under simulated colon fluid for 24 hours. The formula containing MCC, lactose and pectin (5:4:1) with spheronization speed at 1500 rpm for 15 minutes showed the best pellets characteristic and cell viability. The pellets obtained were spherical, with particle size distribution of 913.57±8.28 μm, process yield of 88.71±1.04 % and viability of 7.50 x 107 cfu/g. Pellets coated with CAP showed the highest cell release in simulated colon fluid of 1.38 x 107 CFU/g at 24 hours. This research proved that CAP coated pellet formulation has promising potential for colon targeted delivery of L. plantarum as well as to protect the viability of probiotics for colon-targeted delivery.
References
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Singh, A., Mandal, U. K., & Narang, R. K. (2021). Development and characterization of enteric coated pectin pellets containing mesalamine and Saccharomyces boulardii for specific inflamed colon: In vitro and in vivo evaluation. Journal of Drug Delivery Science and Technology, 62(January), 102393. https://doi.org/10.1016/j.jddst.2021.102393
Sinha, V. R., Agrawal, M. K., Kumria, R., & Bhinge, J. R. (2007). Influence of operational variables on properties of piroxicam pellets prepared by extrusion-spheronization: A technical note. AAPS PharmSciTech, 8(1), E137–E141. https://doi.org/10.1208/pt0801020
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Zheng, H., Gao, M., Ren, Y., Lou, R., Xie, H., Yu, W., Liu, X., & Ma, X. (2017). An improved pH-responsive carrier based on EDTA-Ca-alginate for oral delivery of Lactobacillus rhamnosus ATCC 53103. Carbohydrate Polymers, 155, 329–335. https://doi.org/10.1016/j.carbpol.2016.08.096
Bron, P. A., Van Baarlen, P., & Kleerebezem, M. (2012). Emerging molecular insights into the interaction between probiotics and the host intestinal mucosa. Nature Reviews Microbiology, 10(1), 66–78. https://doi.org/10.1038/nrmicro2690
Chopra, S., Venkatesan, N., & Betageri, G. V. (2013). Formulation of lipid bearing pellets as a delivery system for poorly soluble drugs. International Journal of Pharmaceutics, 446(1–2), 136–144. https://doi.org/10.1016/j.ijpharm.2013.02.029
Darmastuti, A., Hasan, P. N., Wikandari, R., Utami, T., Rahayu, E. S., & Suroto, D. A. (2021). Adhesion properties of lactobacillus plantarum dad-13 and lactobacillus plantarum mut-7 on sprague dawley rat intestine. Microorganisms, 9(11). https://doi.org/10.3390/microorganisms9112336
Fu, B., Huang, X., Ma, J., Chen, Q., Zhang, Q., & Yu, P. (2022). Characterization of an inositol-producing Lactobacillus plantarum strain and the assessment of its probiotic potential and antibacterial activity. Lwt, 153(September 2021), 112553. https://doi.org/10.1016/j.lwt.2021.112553
Gately, N. M., & Kennedy, J. E. (2017). The development of a melt-extruded shellac carrier for the targeted delivery of probiotics to the colon. Pharmaceutics, 9(4), 1–12. https://doi.org/10.3390/pharmaceutics9040038
Jubie, S., Jawahar, N., Arigo, A., Prabha, T., & Anjali, P. B. (2020). Stability enhancement and formulation development of L-Carnitine fast-dissolving pellets through pro-drug strategy. Journal of Drug Delivery Science and Technology, 55. https://doi.org/10.1016/j.jddst.2019.101474
Kaur, V., Goyal, A. K., Ghosh, G., Chandra Si, S., & Rath, G. (2020). Development and characterization of pellets for targeted delivery of 5-fluorouracil and phytic acid for treatment of colon cancer in Wistar rat. Heliyon, 6(1). https://doi.org/10.1016/j.heliyon.2019.e03125
Kumar, A., Gulati, M., Singh, S. K., Gowthamarajan, K., Prashar, R., Mankotia, D., Gupta, J. P., Banerjee, M., Sinha, S., Awasthi, A., Corrie, L., Kumar, R., Patni, P., Kumar, B., Pandey, N. K., Sadotra, M., Kumar, P., Kumar, R., Wadhwa, S., & Khursheed, R. (2020). Effect of co-administration of probiotics with guar gum, pectin and eudragit S100 based colon targeted mini tablets containing 5-Fluorouracil for site specific release. Journal of Drug Delivery Science and Technology, 60(April), 102004. https://doi.org/10.1016/j.jddst.2020.102004
Kurakula, M., Gorityala, S., & Moharir, K. (2021). Recent trends in design and evaluation of chitosan-based colon targeted drug delivery systems: Update 2020. Journal of Drug Delivery Science and Technology, 64(December 2020), 102579. https://doi.org/10.1016/j.jddst.2021.102579
Liwan, S. Y., Utami, T., Murdiati, A., Triwitono, P., & Rahayu, E. S. (2020). Dietary patterns and effect of consumption of probiotic powder containing indigenous bacteria Lactobacillus plantarum Dad-13 on Streptococcus, Enterococcus, Escherichia coli and Klebsiella pneumoniae in the gut of students at Junior High School Pangururan. International Food Research Journal, 27(5), 790–797.
Maghfirotin Marta, B., Tyas, U., Muhammad Nur, C., Jaka, W., & Endang Sutriswati, R. (2019). Effects of Consumption of Probiotic Powder Containing Lactobacillus Plantarum Dad-13 on Fecal Bacterial Population in School-Age Children in Indonesia . International Journal of Probiotics and Prebiotics, 14(1), 1–8. https://doi.org/10.37290/ijpp2641-7197.14:1-8
Noreen, A., Nazli, Z. i. H., Akram, J., Rasul, I., Mansha, A., Yaqoob, N., Iqbal, R., Tabasum, S., Zuber, M., & Zia, K. M. (2017). Pectins functionalized biomaterials; a new viable approach for biomedical applications: A review. International Journal of Biological Macromolecules, 101, 254–272. https://doi.org/10.1016/j.ijbiomac.2017.03.029
Plaza-Diaz, J., Ruiz-Ojeda, F. J., Gil-Campos, M., & Gil, A. (2019). Mechanisms of Action of Probiotics. Advances in Nutrition, 10, S49–S66. https://doi.org/10.1093/advances/nmy063
Prudhviraj, G., Vaidya, Y., Singh, S. K., Yadav, A. K., Kaur, P., Gulati, M., & Gowthamarajan, K. (2015). Effect of co-administration of probiotics with polysaccharide based colon targeted delivery systems to optimize site specific drug release. European Journal of Pharmaceutics and Biopharmaceutics, 97, 164–172. https://doi.org/10.1016/j.ejpb.2015.09.012
Qin, X. S., Luo, Z. G., & Li, X. L. (2021). An enhanced pH-sensitive carrier based on alginate-Ca-EDTA in a set-type W1/O/W2 double emulsion model stabilized with WPI-EGCG covalent conjugates for probiotics colon-targeted release. Food Hydrocolloids, 113(June 2020), 106460. https://doi.org/10.1016/j.foodhyd.2020.106460
Raise, A., Dupont, S., Iaconelli, C., Caliri, C., Charriau, A., Gervais, P., Chambin, O., & Beney, L. (2020). Comparison of two encapsulation processes to protect the commensal gut probiotic bacterium Faecalibacterium prausnitzii from the digestive tract. Journal of Drug Delivery Science and Technology, 56(October 2019), 101608. https://doi.org/10.1016/j.jddst.2020.101608
Sampath Udeni Gunathilake, T. M., Ching, Y. C., Chuah, C. H., Rahman, N. A., & Liou, N. S. (2020). Recent advances in celluloses and their hybrids for stimuli-responsive drug delivery. International Journal of Biological Macromolecules, 158, 670–688. https://doi.org/10.1016/j.ijbiomac.2020.05.010
Shahdadi Sardo, H., Saremnejad, F., Bagheri, S., Akhgari, A., Afrasiabi Garekani, H., & Sadeghi, F. (2019). A review on 5-aminosalicylic acid colon-targeted oral drug delivery systems. In International Journal of Pharmaceutics (Vol. 558, pp. 367–379). Elsevier B.V. https://doi.org/10.1016/j.ijpharm.2019.01.022
Singh, A., Mandal, U. K., & Narang, R. K. (2021). Development and characterization of enteric coated pectin pellets containing mesalamine and Saccharomyces boulardii for specific inflamed colon: In vitro and in vivo evaluation. Journal of Drug Delivery Science and Technology, 62(January), 102393. https://doi.org/10.1016/j.jddst.2021.102393
Sinha, V. R., Agrawal, M. K., Kumria, R., & Bhinge, J. R. (2007). Influence of operational variables on properties of piroxicam pellets prepared by extrusion-spheronization: A technical note. AAPS PharmSciTech, 8(1), E137–E141. https://doi.org/10.1208/pt0801020
Sun, Q., & Wicker, L. (2021). Hydrogel encapsulation of lactobacillus casei by block charge modified pectin and improved gastric and storage stability. Foods, 10(6), 1–10. https://doi.org/10.3390/foods10061337
Torp, A. M., Bahl, M. I., Boisen, A., & Licht, T. R. (2022). Optimizing oral delivery of next generation probiotics. Trends in Food Science & Technology, 119(December 2021), 101–109. https://doi.org/10.1016/j.tifs.2021.11.034
Utami, T., Wardani, S. K., Cahyanto, M. N., & Rahayu, E. S. (2017). The effect of inoculum size and incubation temperature on cell growth , acid production and curd formation during milk fermentation by Lactobacillus plantarum Dad 13. International Food Research Journal, 24(June), 921–926. http://www.ifrj.upm.edu.my
Wang, M., Hao, W., Zhang, L., Zhu, Y., Chen, K., Ma, S., Cheng, X., & Zhao, J. (2021). Lipid-polymer nano core-shell type hybrid system for colon specific drug delivery. Journal of Drug Delivery Science and Technology, 63(January), 102540. https://doi.org/10.1016/j.jddst.2021.102540
Zheng, H., Gao, M., Ren, Y., Lou, R., Xie, H., Yu, W., Liu, X., & Ma, X. (2017). An improved pH-responsive carrier based on EDTA-Ca-alginate for oral delivery of Lactobacillus rhamnosus ATCC 53103. Carbohydrate Polymers, 155, 329–335. https://doi.org/10.1016/j.carbpol.2016.08.096
Published
2024-03-25
How to Cite
I Dewa Gede, A. A., Iswandana, R., & Hartrianti, P. (2024). Development and Characterization of Pectin-Based Colon Targeted Pellets Containing Lactobacillus Plantarum FNCC-0461. Indonesian Journal of Pharmacy, 35(1), 93-104. https://doi.org/10.22146/ijp.8180
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Section
Research Article
