Apium graveolens Herbs Ethanolic-Extract Improve Acetic Acid–Induced Colitis Condition in Rats
Keywords:
Apium graveolens, ethanolic-extract, colitis, Enterobacteriaceae, Inflammation
Abstract
Colitis is a growing gastrointestinal inflammation condition that affect individual all around the globe. No specific treatment for colitis condition is available at the moment and this disease can develop into colorectal cancer. Phytochemical in Apium graveolens (AG) are potential as anti-inflammation and antioxidant. This study aimed to investigate the potency of AG ethanolic-extract (EES) in improving acetic acid (AA) – induced colitis in rats.
Rats with colitis induced by acetic acid were treated with 5-ASA and several concentrations (100, 300, and 900 mg/kg) of EES. Colitis severity was determined by scoring of macroscopic condition including body weight, colon, and faeces condition. In addition, colon tissues conditions were observed with haematoxylin-eosin staining. Colon permeability is determined by measuring urine glucose level. The observation of the change in Enterobacteriaceae population is investigated using PCR.
Apium graveolens ethanolic-extract shows highest improvement of colitis condition at concentration of 900 mg/kg, based on macroscopic observation and disease severity index. At this dose, no blood existed on the faeces and the faeces consistency is solid. Group receiving lower dose of EES still shows blood existence and less solid faeces. The EES dose dependently return the population of Enterobacteriaceae which increase upon AA treatment. Apium graveolens is potential to be use as preventive or treatment for colitis.
References
Alatab, S., Sepanlou, S. G., Ikuta, K., Vahedi, H., Bisignano, C., Safiri, S., Sadeghi, A., Nixon, M. R., Abdoli, A., Abolhassani, H., Alipour, V., Almadi, M. A. H., Almasi-Hashiani, A., Anushiravani, A., Arabloo, J., Atique, S., Awasthi, A., Badawi, A., Baig, A. A. A., … Naghavi, M. (2020). The global, regional, and national burden of inflammatory bowel disease in 195 countries and territories, 1990–2017: A systematic analysis for the Global Burden of Disease Study 2017. The Lancet Gastroenterology & Hepatology, 5(1), 17–30. https://doi.org/10.1016/S2468-1253(19)30333-4
Baldelli, V., Scaldaferri, F., Putignani, L., & Del Chierico, F. (2021). The Role of Enterobacteriaceae in Gut Microbiota Dysbiosis in Inflammatory Bowel Diseases. Microorganisms, 9(4), 697. https://doi.org/10.3390/microorganisms9040697
Butter, M., Weiler, S., Biedermann, L., Scharl, M., Rogler, G., Bischoff-Ferrari, H. A., & Misselwitz, B. (2018). Clinical manifestations, pathophysiology, treatment and outcome of inflammatory bowel diseases in older people. Maturitas, 110, 71–78. https://doi.org/10.1016/j.maturitas.2018.01.015
Chang, C.-C., Yang, M.-H., Wen, H.-M., & Chern, J.-C. (2020). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3). https://doi.org/10.38212/2224-6614.2748
Chang, M., Chang, L., Chang, H. M., & Chang, F. (2018). Intestinal and Extraintestinal Cancers Associated With Inflammatory Bowel Disease. Clinical Colorectal Cancer, 17(1), e29–e37. https://doi.org/10.1016/j.clcc.2017.06.009
Chapman, J., Goyal, A., & Azevedo, A. M. (2021). Splenomegaly. In StatPearls. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK430907/
Chemat, F., Rombaut, N., Sicaire, A.-G., Meullemiestre, A., Fabiano-Tixier, A.-S., & Abert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34, 540–560. https://doi.org/10.1016/j.ultsonch.2016.06.035
Chua, L. S. (2013). A review on plant-based rutin extraction methods and its pharmacological activities. Journal of Ethnopharmacology, 150(3), 805–817. https://doi.org/10.1016/j.jep.2013.10.036
Derouich, M., Bouhlali, E. D. T., Hmidani, A., Bammou, M., Bourkhis, B., Sellam, K., & Alem, C. (2020). Assessment of total polyphenols, flavonoids and anti-inflammatory potential of three Apiaceae species grown in the Southeast of Morocco. Scientific African, 9, e00507. https://doi.org/10.1016/j.sciaf.2020.e00507
Fatani, A. J., Alrojayee, F. S., Parmar, M. Y., Abuohashish, H. M., Ahmed, M. M., & Al-Rejaie, S. S. (2016). Myrrh attenuates oxidative and inflammatory processes in acetic acid-induced ulcerative colitis. Experimental and Therapeutic Medicine, 12(2), 730–738. https://doi.org/10.3892/etm.2016.3398
Fazzeli, H., Arabestani, M. R., Esfahani, B. N., Khorvash, F., Pourshafie, M. R., Moghim, S., Safaei, H. G., Faghri, J., & Narimani, T. (2012). Development of PCR-based method for detection of Enterobacteriaceae in septicemia. Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences, 17(7), 671–675.
Gentile, D., Fornai, M., Colucci, R., Pellegrini, C., Tirotta, E., Benvenuti, L., Segnani, C., Ippolito, C., Duranti, E., Virdis, A., Carpi, S., Nieri, P., Németh, Z. H., Pistelli, L., Bernardini, N., Blandizzi, C., & Antonioli, L. (2018). The flavonoid compound apigenin prevents colonic inflammation and motor dysfunctions associated with high fat diet-induced obesity. PLOS ONE, 13(4), e0195502. https://doi.org/10.1371/journal.pone.0195502
Ghattamaneni, N. K. R. (2019). An improved rat model for chronic inflammatory bowel disease. Pharmacological Reports, 7.
Hanci, V., Gülle, K., Karakaya, K., Yurtlu, S., Akpolat, M., Yüce, M. F., Yüce, F. Z., & Turan, I. Ö. (2015). Dexmedetomidina retal em ratos: Avaliação dos efeitos sedativos e sobre a mucosa. Brazilian Journal of Anesthesiology, 65(1), 1–6. https://doi.org/10.1016/j.bjan.2013.09.004
Jazayeri, O., Daghighi, S. M., & Rezaee, F. (2017). Lifestyle alters GUT-bacteria function: Linking immune response and host. Best Practice & Research Clinical Gastroenterology, 31(6), 625–635. https://doi.org/10.1016/j.bpg.2017.09.009
Jeengar, M. K., Thummuri, D., Magnusson, M., Naidu, V. G. M., & Uppugunduri, S. (2017). Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice. Scientific Reports, 7(1), 3924. https://doi.org/10.1038/s41598-017-04041-9
Kahai, P., Mandiga, P., Wehrle, C. J., & Lobo, S. (2021). Anatomy, Abdomen and Pelvis, Large Intestine. In StatPearls. StatPearls Publishing. http://www.ncbi.nlm.nih.gov/books/NBK470577/
Kawashima, K., Onizawa, M., Fujiwara, T., Gunji, N., Imamura, H., Katakura, K., & Ohira, H. (2022). Evaluation of the relationship between the spleen volume and the disease activity in ulcerative colitis and Crohn disease. Medicine, 101(1), e28515. https://doi.org/10.1097/MD.0000000000028515
Kooti, W., Ali-Akbari, S., Asadi-Samani, M., Ghadery, H., & Ashtary-Larky, D. (2014). A review on medicinal plant of Apium graveolens.
Li, S., Li, L., Yan, H., Jiang, X., Hu, W., Han, N., & Wang, D. (2019). Anti‑gouty arthritis and anti‑hyperuricemia properties of celery seed extracts in rodent models. Molecular Medicine Reports. https://doi.org/10.3892/mmr.2019.10708
Liu, D.-K., Xu, C.-C., Zhang, L., Ma, H., Chen, X.-J., Sui, Y.-C., & Zhang, H.-Z. (2020). Evaluation of bioactive components and antioxidant capacity of four celery ( Apium graveolens L.) leaves and petioles. International Journal of Food Properties, 23(1), 1097–1109. https://doi.org/10.1080/10942912.2020.1778027
Magro, F., Gionchetti, P., Eliakim, R., Ardizzone, S., Armuzzi, A., Barreiro-de Acosta, M., Burisch, J., Gecse, K. B., Hart, A. L., Hindryckx, P., Langner, C., Limdi, J. K., Pellino, G., Zagórowicz, E., Raine, T., Harbord, M., Rieder, F., & for the European Crohn’s and Colitis Organisation [ECCO]. (2017). Third European Evidence-based Consensus on Diagnosis and Management of Ulcerative Colitis. Part 1: Definitions, Diagnosis, Extra-intestinal Manifestations, Pregnancy, Cancer Surveillance, Surgery, and Ileo-anal Pouch Disorders. Journal of Crohn’s and Colitis, 11(6), 649–670. https://doi.org/10.1093/ecco-jcc/jjx008
Minaiyan, M., Asghari, G., Taheri, D., Saeidi, M., & Nasr-Esfahani, S. (2014). Anti-inflammatory effect of Moringa oleifera Lam. Seeds on acetic acid-induced acute colitis in rats. Avicenna Journal of Phytomedicine, 4(2), 127–136.
Minaiyan, M., Ghanadian, S.-M., & Hossaini, M. (2021). Protective Effect of Apium graveolens L. (Celery) Seeds Extracts and Luteolin on Acetic Acid-Induced Colitis in Rats. International Journal of Preventive Medicine, 12, 100. https://doi.org/10.4103/ijpvm.IJPVM_651_20
Nascimento, R. de P. do, Machado, A. P. da F., Galvez, J., Cazarin, C. B. B., & Maróstica Junior, M. R. (2020). Ulcerative colitis: Gut microbiota, immunopathogenesis and application of natural products in animal models. Life Sciences, 258, 118129. https://doi.org/10.1016/j.lfs.2020.118129
Radulovic, K., Normand, S., Rehman, A., Delanoye-Crespin, A., Chatagnon, J., Delacre, M., Waldschmitt, N., Poulin, L. F., Iovanna, J., Ryffel, B., Rosenstiel, P., & Chamaillard, M. (2018). A dietary flavone confers communicable protection against colitis through NLRP6 signaling independently of inflammasome activation. Mucosal Immunology, 11(3), 811–819. https://doi.org/10.1038/mi.2017.87
Soliman, M. M., Nassan, M. A., Aldhahrani, A., Althobaiti, F., & Mohamed, W. A. (2020). Molecular and Histopathological Study on the Ameliorative Impacts of Petroselinum Crispum and Apium Graveolens against Experimental Hyperuricemia. Scientific Reports, 10(1), 9512. https://doi.org/10.1038/s41598-020-66205-4
Sovran, B., Planchais, J., Jegou, S., Straube, M., Lamas, B., Natividad, J. M., Agus, A., Dupraz, L., Glodt, J., Da Costa, G., Michel, M.-L., Langella, P., Richard, M. L., & Sokol, H. (2018). Enterobacteriaceae are essential for the modulation of colitis severity by fungi. Microbiome, 6(1), 152. https://doi.org/10.1186/s40168-018-0538-9
Sukketsiri, W., Chonpathompikunlert, P., Tanasawet, S., Choosri, N., & Wongtawatchai, T. (2016). Effects of Apium graveolens Extract on the Oxidative Stress in the Liver of Adjuvant-Induced Arthritic Rats. Preventive Nutrition and Food Science, 21(2), 79–84. https://doi.org/10.3746/pnf.2016.21.2.79
Tian, Z., Liu, J., Liao, M., Li, W., Zou, J., Han, X., Kuang, M., Shen, W., & Li, H. (2016). Beneficial Effects of Fecal Microbiota Transplantation on Ulcerative Colitis in Mice. Digestive Diseases and Sciences, 61(8), 2262–2271. https://doi.org/10.1007/s10620-016-4060-2
Uranga, J. A., López-Miranda, V., Lombó, F., & Abalo, R. (2016). Food, nutrients and nutraceuticals affecting the course of inflammatory bowel disease. Pharmacological Reports, 68(4), 816–826. https://doi.org/10.1016/j.pharep.2016.05.002
Wei, M., & Yang, L. (2017). Determination of orientin in Trollius chinensis using ultrasound-assisted extraction and high performance liquid chromatography: Several often-overlooked sample preparation parameters in an ultrasonic bath. Journal of Chromatography A, 1530, 68–79. https://doi.org/10.1016/j.chroma.2017.11.034
Baldelli, V., Scaldaferri, F., Putignani, L., & Del Chierico, F. (2021). The Role of Enterobacteriaceae in Gut Microbiota Dysbiosis in Inflammatory Bowel Diseases. Microorganisms, 9(4), 697. https://doi.org/10.3390/microorganisms9040697
Butter, M., Weiler, S., Biedermann, L., Scharl, M., Rogler, G., Bischoff-Ferrari, H. A., & Misselwitz, B. (2018). Clinical manifestations, pathophysiology, treatment and outcome of inflammatory bowel diseases in older people. Maturitas, 110, 71–78. https://doi.org/10.1016/j.maturitas.2018.01.015
Chang, C.-C., Yang, M.-H., Wen, H.-M., & Chern, J.-C. (2020). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3). https://doi.org/10.38212/2224-6614.2748
Chang, M., Chang, L., Chang, H. M., & Chang, F. (2018). Intestinal and Extraintestinal Cancers Associated With Inflammatory Bowel Disease. Clinical Colorectal Cancer, 17(1), e29–e37. https://doi.org/10.1016/j.clcc.2017.06.009
Chapman, J., Goyal, A., & Azevedo, A. M. (2021). Splenomegaly. In StatPearls. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK430907/
Chemat, F., Rombaut, N., Sicaire, A.-G., Meullemiestre, A., Fabiano-Tixier, A.-S., & Abert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34, 540–560. https://doi.org/10.1016/j.ultsonch.2016.06.035
Chua, L. S. (2013). A review on plant-based rutin extraction methods and its pharmacological activities. Journal of Ethnopharmacology, 150(3), 805–817. https://doi.org/10.1016/j.jep.2013.10.036
Derouich, M., Bouhlali, E. D. T., Hmidani, A., Bammou, M., Bourkhis, B., Sellam, K., & Alem, C. (2020). Assessment of total polyphenols, flavonoids and anti-inflammatory potential of three Apiaceae species grown in the Southeast of Morocco. Scientific African, 9, e00507. https://doi.org/10.1016/j.sciaf.2020.e00507
Fatani, A. J., Alrojayee, F. S., Parmar, M. Y., Abuohashish, H. M., Ahmed, M. M., & Al-Rejaie, S. S. (2016). Myrrh attenuates oxidative and inflammatory processes in acetic acid-induced ulcerative colitis. Experimental and Therapeutic Medicine, 12(2), 730–738. https://doi.org/10.3892/etm.2016.3398
Fazzeli, H., Arabestani, M. R., Esfahani, B. N., Khorvash, F., Pourshafie, M. R., Moghim, S., Safaei, H. G., Faghri, J., & Narimani, T. (2012). Development of PCR-based method for detection of Enterobacteriaceae in septicemia. Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences, 17(7), 671–675.
Gentile, D., Fornai, M., Colucci, R., Pellegrini, C., Tirotta, E., Benvenuti, L., Segnani, C., Ippolito, C., Duranti, E., Virdis, A., Carpi, S., Nieri, P., Németh, Z. H., Pistelli, L., Bernardini, N., Blandizzi, C., & Antonioli, L. (2018). The flavonoid compound apigenin prevents colonic inflammation and motor dysfunctions associated with high fat diet-induced obesity. PLOS ONE, 13(4), e0195502. https://doi.org/10.1371/journal.pone.0195502
Ghattamaneni, N. K. R. (2019). An improved rat model for chronic inflammatory bowel disease. Pharmacological Reports, 7.
Hanci, V., Gülle, K., Karakaya, K., Yurtlu, S., Akpolat, M., Yüce, M. F., Yüce, F. Z., & Turan, I. Ö. (2015). Dexmedetomidina retal em ratos: Avaliação dos efeitos sedativos e sobre a mucosa. Brazilian Journal of Anesthesiology, 65(1), 1–6. https://doi.org/10.1016/j.bjan.2013.09.004
Jazayeri, O., Daghighi, S. M., & Rezaee, F. (2017). Lifestyle alters GUT-bacteria function: Linking immune response and host. Best Practice & Research Clinical Gastroenterology, 31(6), 625–635. https://doi.org/10.1016/j.bpg.2017.09.009
Jeengar, M. K., Thummuri, D., Magnusson, M., Naidu, V. G. M., & Uppugunduri, S. (2017). Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice. Scientific Reports, 7(1), 3924. https://doi.org/10.1038/s41598-017-04041-9
Kahai, P., Mandiga, P., Wehrle, C. J., & Lobo, S. (2021). Anatomy, Abdomen and Pelvis, Large Intestine. In StatPearls. StatPearls Publishing. http://www.ncbi.nlm.nih.gov/books/NBK470577/
Kawashima, K., Onizawa, M., Fujiwara, T., Gunji, N., Imamura, H., Katakura, K., & Ohira, H. (2022). Evaluation of the relationship between the spleen volume and the disease activity in ulcerative colitis and Crohn disease. Medicine, 101(1), e28515. https://doi.org/10.1097/MD.0000000000028515
Kooti, W., Ali-Akbari, S., Asadi-Samani, M., Ghadery, H., & Ashtary-Larky, D. (2014). A review on medicinal plant of Apium graveolens.
Li, S., Li, L., Yan, H., Jiang, X., Hu, W., Han, N., & Wang, D. (2019). Anti‑gouty arthritis and anti‑hyperuricemia properties of celery seed extracts in rodent models. Molecular Medicine Reports. https://doi.org/10.3892/mmr.2019.10708
Liu, D.-K., Xu, C.-C., Zhang, L., Ma, H., Chen, X.-J., Sui, Y.-C., & Zhang, H.-Z. (2020). Evaluation of bioactive components and antioxidant capacity of four celery ( Apium graveolens L.) leaves and petioles. International Journal of Food Properties, 23(1), 1097–1109. https://doi.org/10.1080/10942912.2020.1778027
Magro, F., Gionchetti, P., Eliakim, R., Ardizzone, S., Armuzzi, A., Barreiro-de Acosta, M., Burisch, J., Gecse, K. B., Hart, A. L., Hindryckx, P., Langner, C., Limdi, J. K., Pellino, G., Zagórowicz, E., Raine, T., Harbord, M., Rieder, F., & for the European Crohn’s and Colitis Organisation [ECCO]. (2017). Third European Evidence-based Consensus on Diagnosis and Management of Ulcerative Colitis. Part 1: Definitions, Diagnosis, Extra-intestinal Manifestations, Pregnancy, Cancer Surveillance, Surgery, and Ileo-anal Pouch Disorders. Journal of Crohn’s and Colitis, 11(6), 649–670. https://doi.org/10.1093/ecco-jcc/jjx008
Minaiyan, M., Asghari, G., Taheri, D., Saeidi, M., & Nasr-Esfahani, S. (2014). Anti-inflammatory effect of Moringa oleifera Lam. Seeds on acetic acid-induced acute colitis in rats. Avicenna Journal of Phytomedicine, 4(2), 127–136.
Minaiyan, M., Ghanadian, S.-M., & Hossaini, M. (2021). Protective Effect of Apium graveolens L. (Celery) Seeds Extracts and Luteolin on Acetic Acid-Induced Colitis in Rats. International Journal of Preventive Medicine, 12, 100. https://doi.org/10.4103/ijpvm.IJPVM_651_20
Nascimento, R. de P. do, Machado, A. P. da F., Galvez, J., Cazarin, C. B. B., & Maróstica Junior, M. R. (2020). Ulcerative colitis: Gut microbiota, immunopathogenesis and application of natural products in animal models. Life Sciences, 258, 118129. https://doi.org/10.1016/j.lfs.2020.118129
Radulovic, K., Normand, S., Rehman, A., Delanoye-Crespin, A., Chatagnon, J., Delacre, M., Waldschmitt, N., Poulin, L. F., Iovanna, J., Ryffel, B., Rosenstiel, P., & Chamaillard, M. (2018). A dietary flavone confers communicable protection against colitis through NLRP6 signaling independently of inflammasome activation. Mucosal Immunology, 11(3), 811–819. https://doi.org/10.1038/mi.2017.87
Soliman, M. M., Nassan, M. A., Aldhahrani, A., Althobaiti, F., & Mohamed, W. A. (2020). Molecular and Histopathological Study on the Ameliorative Impacts of Petroselinum Crispum and Apium Graveolens against Experimental Hyperuricemia. Scientific Reports, 10(1), 9512. https://doi.org/10.1038/s41598-020-66205-4
Sovran, B., Planchais, J., Jegou, S., Straube, M., Lamas, B., Natividad, J. M., Agus, A., Dupraz, L., Glodt, J., Da Costa, G., Michel, M.-L., Langella, P., Richard, M. L., & Sokol, H. (2018). Enterobacteriaceae are essential for the modulation of colitis severity by fungi. Microbiome, 6(1), 152. https://doi.org/10.1186/s40168-018-0538-9
Sukketsiri, W., Chonpathompikunlert, P., Tanasawet, S., Choosri, N., & Wongtawatchai, T. (2016). Effects of Apium graveolens Extract on the Oxidative Stress in the Liver of Adjuvant-Induced Arthritic Rats. Preventive Nutrition and Food Science, 21(2), 79–84. https://doi.org/10.3746/pnf.2016.21.2.79
Tian, Z., Liu, J., Liao, M., Li, W., Zou, J., Han, X., Kuang, M., Shen, W., & Li, H. (2016). Beneficial Effects of Fecal Microbiota Transplantation on Ulcerative Colitis in Mice. Digestive Diseases and Sciences, 61(8), 2262–2271. https://doi.org/10.1007/s10620-016-4060-2
Uranga, J. A., López-Miranda, V., Lombó, F., & Abalo, R. (2016). Food, nutrients and nutraceuticals affecting the course of inflammatory bowel disease. Pharmacological Reports, 68(4), 816–826. https://doi.org/10.1016/j.pharep.2016.05.002
Wei, M., & Yang, L. (2017). Determination of orientin in Trollius chinensis using ultrasound-assisted extraction and high performance liquid chromatography: Several often-overlooked sample preparation parameters in an ultrasonic bath. Journal of Chromatography A, 1530, 68–79. https://doi.org/10.1016/j.chroma.2017.11.034
Published
2024-06-10
How to Cite
Sahid, M. N. A., Dewangga, A., Saputra, C., & Gani, A. P. (2024). Apium graveolens Herbs Ethanolic-Extract Improve Acetic Acid–Induced Colitis Condition in Rats. Indonesian Journal of Pharmacy, 35(2), 239-249. https://doi.org/10.22146/ijp.7158
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Research Article
