Mangosteen Garcinia mangostana L. Simplicia Effect on Bone Structure and Behaviour of Wader Fish Rasbora lateristriata (Bleeker, 1854) Embryo
Keywords:
Bone structure, Mangosteen peel extract, Rasbora lateristriata, Toxicity
Abstract
Mangosteen (Garcinia mangostana L.) is a fruit originating from the tropics which belongs to the Clusiaceae family and has become a commodity with many enthusiasts from various countries. Previous research has shown that mangosteen peel extract has tremendous potential to be developed as an ingredient in herbal medicine. Mangosteen fruit contains anti-inflammatory, antibacterial, antifungal, antihistamine, antidiabetic, anticancer, and so on. The results of the phytochemical screening test of 95% ethanol extract on mangosteen rind showed that it contained flavonoid compounds, saponins, alkaloids, triterpenoids, tannins and polyphenols. There is no data on the effect of exposure to mangosteen pericarip simplicia, therefore it is necessary to study the effect of exposure to mangosteen peel decoction on animal models of Wader Pari Fish (Rasbora lateristriata), with parameters such as morphology and bone structure in larvae wader fish (Rasbora lateristriata). Alcian Blue Alizarin Red staining were used to study bone structure in wader fish (Rasbora lateristriata). Data were statistical analysis with SPSS ver. 25 and the significance was determined using one-way analysis of variance (ANOVA). The morphological examination results revealed that the embryo and larvae had no morphological abnormalities. The cranial cartilage examination also revealed that the larvae had no abnormalities, the length and the angle of the cranial cartilage did not change between the control and mangosteen pericarp simplicia treatment.
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David, N.B., Saint-Etienne, L., Tsang, M., Schilling, T.F. and Rosa, F.M. 2002. Requirement for endoderm and FGF3 in ventral head skeleton formation. Development, 129: 4457-4468.
Fazry, S., M.A.M. Noordin, S. Sanusi, M.M. Noor, W.M. Aizat, A.M. Lazim, H.R.W. Dyari, N.H. Jamar, J. Remali, B. A. Othman, D. Law, N.M. Sidik, Y.H. Cheah, & Y.C. Lim. 2018. Cytotoxicity and toxicity evaluation of xanthone crude extract on hypoxic human hepatocellular carcinoma and zebrafish (Danio rario) embryos. Toxics, 6(60): 1-10.
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George, P. 2011. Concerns regarding the safety and toxicity of medical plants – An overview. Journal of Applied Pharmaceutical Science, 01(06): 40-44.
Good, C., Davidson, J., Kinman, C., Kenney, P.B., Baevaerfjord, G. and Summerfelt, S. 2014. Observations on side-swimming rainbow trout in water recirculation aquaculture system. Journal of Aquatic Animal Health, 26: 219-224.
Gutierrez-Orozco, F., C. Chitchumroonchokchai, G.B. Lesinski, S. Suksamrarn, & M.L. Failla. 2013. α-Mangostin: anti-inflamatory activity and metabolism by human cells. Journal of Agricultural and Food Chemistry, 61(16): 3891-3900.
Gutzman, J.H., Graeden, E.G., Lowery, L.A., Holley, H.S. and Sive, H. 2008. Formation of the zebrafish midbrain-hindbrain boundary constriction requires laminin-dependent basal constriction. Mechanism of Develelopment, 125(11-12): 974-983.
Hamid, M.A., Sarmidi, M.R. and Park, C.S. 2012. Mangosteen leaf extract increases melanogenesis in B16F1 melanoma cells by stimulating tyrosinase activity in vitro and by up-regulating tyrosinase gene expression. International Journal of Molecular Medicine, 29: 209-217.
Hoyberghs, J., C. Bars, C. Pype, K. Foubert, M.A. Hernando, C.V. Ginneke, J. Ball, & s. v. Cruchten. 2020. Refinement of the zebrafish embryo developmental toxicity assay. MethodsX, 7: 1-14.
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Hultman, K.A., Bahary, N., Zon, L.I. and Johnson, S.L. Gene duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand α. PLOS Genetics, 3(1): 89-102.
Kitipaspallop, W., S. Sillapaprayoon, P. Taepavarapruk, C. Chanchao, & W. Pimtong. 2021. Evaluation of developmental and transcriptional effects of α-mangostin on zebrafish embryos. Toxicological Environmental Chemistry, 103(3): 254-268.
Knight, R.D. and Schilling, T.F. 2006. Cranial neural crest and development of the head skeleton. Advance in Experimental Medicine and Biology, 589: 120-133.
Kumar, v., P.C. Bhatt, G. Kaithwas, M. Rashid, F.A. Al-Abbasi, J.A.J. Khan, F. Anwar, W. Kittipaspallop, T. Ponnarin, C. Chanchao and W. Pimtong. 2018. Acute toxicity and teratogenicity of α-mangostinin in zebrafish embryos. Exp Biol Med (Maywood), 243 (15-16): 1212-1219.
Mustapa, M.A., T.S. Tuloli and A. Mooduto. 2018. Uji toksisitas akut yang diukur dengan penetuan Ld50 ekstrak etanol bunga cengkeh (Syzygium aromaticum L.) terhadap mencit (Mus musculus) menggunakan metode Thompson-Weil. Frontiers: Jurnal Sains dan Teknologi, 1 (1): 105-117.
Nelli, G.B., K.A.S & E.K. Kilari. 2013. Antidiabetic effect of α-mangostin and its protective role in sexual dysfunction of streptozotocin induced diabetic male rats. Syst Biol Reprod Med 59 (6): 319-328.
Nugroho, A.E. 2009. Manggis (Garcinia mangostana L.): dari Kulit Buah yang Terbuang hingga Menjadi Kandidat Suatu Obat. Laboratorium Farmakologi dan Toksikologi, Bagian Farmakologi dan Farmasi Klinik, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.
Pasaribu, F., P. Sitorus, and S. Bahri. 2012. Uji ekstrak etanol kulit buah manggis (Garcinia mangostana L.) terhadap penurunan kadar glukosa darah. Journal of Pharmaceutics and Pharmacology, 1 (1): 1-8.
Purnomo, A.F. 2015. Pengaruh ekstrak kulit manggis terhadap daya tahan tubuh, laju penetasan dan kecacatan embrio ikan zebra (Danio rerio). Tugas Akhir. Universitas Brawijaya.
Rahajeng, A., & B. Retnoaji. 2021. The effect of dioscorea alata extract on the early development of zebrafish embryo (Danio rerio) and Rasbora lateristriata. Advances in Social Science, Education and Humanities Research, 536: 601-611.
Retnoaji, B., R. Akiyama, T. Matta, Y. Bessho, & T. Matsui. 2014. Retinoic acid controls proper head-to-trunk linkage in zebrafish by regulating and anteroposterior somitogenetic rate difference. Development, 141: 158-165.
Retrieved [March, 13, 2022], from the Integrated Taxonomic Information System (ITIS) on-line databese, www.itis.gov, CC0.
Sandi, F.P., B. Retnoaji, & I.D. Ana. 2022. Effects of carbonate hydroxyapatite (CHA) on the development of heart and cranium cartilage of zebrafish (Danio rerio Hamilton, 1882) larvae. Chiang Mai University Journal of Natural Sciences, 21(3): 1-16.
Singh, A.P. and Nusslein-Volhard, C. 2015. Zebrafish stripes as a model for vertebrate colour pattern formation. Current Biology, 25: R81-R92.
Suwignyo, A. 2014. Uji toksisitas ekstrak etanol kulit manggis (Garcinia mangostana) pada embrio zebrafish (Danio rerio). Sarjana thesis, Universitas Brawijaya.Uji, 2007.
Yelick, P.C. and Schilling, T.F. 2002. Molecular dissection of craniofacial development using zebrafish. Critical Reviews in Oral Biology and Medicine, 13(4): 308-322.
Zakeyudin, M-S., M.M. Isa, C-S. M. Rawi, A-S.M. Shah, & A-H. Ahmad. 2012. Assessment of suitability of Kerian River tributaries using length-weight relationship and relative condition factor of six freshwater fish species. Journal of Environmental and Earth Science, 2(3): 52-60.
Published
2023-04-24
How to Cite
Retnoaji, B., Paramita, P., & Khasanah, L. U. (2023). Mangosteen Garcinia mangostana L. Simplicia Effect on Bone Structure and Behaviour of Wader Fish Rasbora lateristriata (Bleeker, 1854) Embryo. Indonesian Journal of Pharmacy, 34(2), 182-192. https://doi.org/10.22146/ijp.6348
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Section
Research Article
