The Potential of Tapak Dara (Catharanthus roseus) Leaves Endophytic Bacteria BETD5 as Antioxidant and Anticancer Against T47D Breast Cancer Cells

Lenni fitri; Fauziah  Fauziah; Fajar  Dini; Sinta Ayu  Mauludin; Sasmiati Farach  Dita

doi:10.22146/ijp.5667

Lenni fitri universitas syiah kuala
Fauziah Fauziah Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Jln. Teuku Nyak Arief, Darussalam, Banda Aceh, Aceh, 23111, Indonesia
Fajar Dini Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Jln. Teuku Nyak Arief, Darussalam, Banda Aceh, Aceh, 23111, Indonesia
Sinta Ayu Mauludin Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Jln. Teuku Nyak Arief, Darussalam, Banda Aceh, Aceh, 23111, Indonesia
Sasmiati Farach Dita YPPM Mandiri Pharmacy Academy, Jalan Laksamana Malahayati Desa Tibang,No.2 (Taman Kota), Kota Banda Aceh, Indonesia

Keywords: Antioxidant, endophytic bacteria, Cell line T47D, biochemical properties, cytotoxicity.

Abstract

This study aimed to measure antioxidant activity, to characterize biochemical properties and to measure cytotoxicity activity in BETD5 isolates. The method used in this research was experimental method. Parameters observed were antioxidant activity and cytotoxicity activity consisting of IC₅₀ (Inhibition Concentration) and biochemical properties. Isolate used was extract of endophytic bacteria of Tapak Dara (BETD5). antioxidant activity was conducted using various sample extract concentration, namely 5ppm, 10ppm, 25ppm, 50ppm and 100ppm. The biochemical tests carried out included Triple Sugar Iron Agar (TSIA) test, Simmons Citrate (SC) test, motility test, indole test, catalase test and Methyl Red (MR) test. Cytotoxicity test on cell line T47D was performed by MTT assay using concentration (%) of 3.125; 6.25; 12.5; 25 and 50. The results showed that antioxidant activity of BETD5 was strong because IC₅₀ value obtained was 78.485 ppm. Based on biochemical tests, BETD5 was suspected belong to the genus Staphylococcus. Cytotoxicity activity of BETD5 obtained an IC50 value of 15.58 ppm. This indicates that endophytic bacterial extract of Tapak Dara (BETD5) had moderate cytotoxicity against the T47D breast cancer cell line. GCMS test results showed that the endophytic bacterial isolate BETD5 had the highest secondary metabolite content, namely cis-Ocimene (24.31%).

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