Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide

Sri wahdaningsih; Subagus Wahyuono; Sugeng Riyanto; Retno Murwanti

doi:10.22146/ijp.2331

Sri wahdaningsih Departement of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak, Kota Pontianak, Indonesia
Subagus Wahyuono Department of Pharmaceutical Biology Faculty of Pharmacy, Gadjahmada University, Skip Utara Yogyakarta Indonesia, 55281
Sugeng Riyanto Department of Pharmaceutical Chemistry Faculty of Pharmacy, Gadjahmada University, Skip Utara Yogyakarta Indonesia, 55281
Retno Murwanti Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy, Gadjahmada University, Skip Utara Yogyakarta Indonesia, 55281

Keywords: H. polyrhizus, β-sitosterol, macrophage, nitrit oxide

Abstract

Hylocerius polyrhizus has relatively big potency as natural antioxidant. The compound considered as antioxidant also has immunomodulatory activity. This study showed isolation for identifying the active compounds in H. polyrhizus peels that are able to increase immune system of human body. The methanol extracts were partitioned and fractionated. The active compounds of petroleum ether fraction were partitioned and purified using Preparative Thin Layer Chromatography (PTLC). The identification of active compound structures was done with spectroscopy: UV, FT-IR, ¹³CNMR, ¹HNMR, DEPT and HSQC. The immunomodulatory activity was also tested. Based on the spectroscopic data, the identified isolate was β-sitosterol. The statistical analysis of macrophage cell activity and nitric oxide showed that isolates at the highest concentrations of 100 µg/mL were able to activate macrophage cells and enhance the production of nitric oxide.

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