Beetroot Extracts as Haematopoietic Agents on Rats

Ni Putu Ermi  Hikmawanti; Lusi Putri Dwita; Dinitia Az  Zahra

doi:10.22146/ijp.1132

Ni Putu Ermi Hikmawanti Department of Pharmaceutical Biology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, 13460, East Jakarta, Indonesia
Lusi Putri Dwita Department of Pharmacology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, 13460, East Jakarta, Indonesia
Dinitia Az Zahra Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, 13460, East Jakarta, Indonesia

Keywords: Beetroots, Extract, Haematopoietic

Abstract

Introduction: Beetroot (Beta vulgaris L.) contains flavonoid compounds that play a role in the haematopoietic process. It is known that methanol extract of beetroot has benefits in the process of haematopoiesis in normal white rats. Aims: To evaluate the beetroots extracts as hematopoietic agents on male rats. Methods: Beetroots dried powder was divided into two parts. One part was macerated separately with dichloromethane and 70% ethanol, while the other part is added with citric acid and washed with water to remove alkaloids and then extracted with 70% ethanol. The study used 24 rats which were divided into four groups. Each group consisted of 6 rats, namely the normal group, dichloromethane extract group, ethanolic extract group, and free alkaloids-ethanolic extract group. Each extract was given at a dose of 200 mg.Kg^-1 for 21 days. Analyzed blood parameters are erythrocytes, haemoglobin, MCV, MCH, MCHC, leukocytes, and platelets. The data obtained consisted of the number of cells analyzed using one-way ANOVA then obtained by the Tukey test. Results: This study showed a significant increase in the number of erythrocytes, haemoglobin, MCV, MCH, MCHC, leukocytes, and platelets in rats that were given each extract compared to the normal group (p <0.05). The ethanolic extract of beetroot can increase erythrocytes, haemoglobin, MCV, MCH, MCHC, leukocytes, and platelets by 41.49%, 24.95%, 14.92%, 33.54%, 27.19%, 59.40%, and 35.37%, respectively. Conclusions: The ethanolic extract of beetroot has the potential as a good natural haematopoietic agent.

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