Isolation and Evaluation of the Antioxidant Capacity of Compounds from  Ehretia asperula  Zoll. & Moritzi

Chong Kim Thien Duc; Tran Chi Linh; Nguyen Quoc Chau Thanh; Pham Quoc Nhien; Dai Thi Xuan Trang; Luu Thai Danh; Nguyen Trong Tuan

doi:10.22146/ijc.94762

Isolation and Evaluation of the Antioxidant Capacity of Compounds from Ehretia asperula Zoll. & Moritzi

https://doi.org/10.22146/ijc.94762

Chong Kim Thien Duc⁽¹⁾, Tran Chi Linh⁽²⁾, Nguyen Quoc Chau Thanh⁽³⁾, Pham Quoc Nhien⁽⁴⁾, Dai Thi Xuan Trang⁽⁵⁾, Luu Thai Danh⁽⁶⁾, Nguyen Trong Tuan^(7*)

(1) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(2) Department of Biology, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(3) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(4) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(5) Department of Biology, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(6) Department of Agriculture Genetics and Breeding, College of Agriculture, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(7) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(*) Corresponding Author

Abstract

Ehretia asperula Zoll. & Moritzi was a common medicinal herb found in several Asian nations. This herb was found in many provinces in northern Vietnam, especially Hoa Binh province. Leaves of E. asperula cultivated in Tay Ninh Province were used to isolate compounds and evaluate their potential as antioxidants by five methods, including TAC, RP, FRAP, DPPH, and ABTS^•+. Seven compounds were isolated and elucidated from the ethyl acetate fraction, including kaempferol (1), astragalin (2), nicotiflorin (3), rutin (4), caffeic acid (5), (-)-loliolide (6), and daucosterol (7), where compounds 1, 4, 6, and 7 were found from this species for the first time. All isolated compounds from E. asperula leaves exhibited antioxidant activity, consisting of 5.70 ± 0.01 to 299.13 ± 10.19 μg/mL for EC₅₀ values. Especially compound 5 had a very strong antioxidant effect based on five methods TAC (EC₅₀ = 8.43 ± 0.15 μg/mL), RP (EC₅₀ = 6.79 ± 003 μg/mL), FRAP (EC₅₀ = 12.72 ± 006 μg/mL), DPPH (EC₅₀ = 5.57 ± 0.02 µg/mL), and ABTS^•+ (EC₅₀ = 5.70 ± 0.01 μg/mL). From our results, Tay Ninh Province's E. asperula leaves are abundant in naturally occurring antioxidants, indicating their potential use as therapeutic materials.

Keywords

antioxidant; Ehretia asperula; kaempferol; (-)-loliolide; rutin

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DOI: https://doi.org/10.22146/ijc.94762