Toxicity Test of Nanoemulsions of Nutmeg Fruits and Leaves Essential Oil against  Artemia salina  Leach and Its Cytotoxicity Test against Breast Cancer Cells T47D

Undri Rastuti; Hartiwi Diastuti; Senny Widyaningsih; Moch Chasani; Cindi Sheiliyani; Anisa Rahmasari; Bunga Sita Roihanul Fajriyah; Puspa Rahma Mesayu; Ranti Kamila Habibie

doi:10.22146/ijc.91077

Toxicity Test of Nanoemulsions of Nutmeg Fruits and Leaves Essential Oil against Artemia salina Leach and Its Cytotoxicity Test against Breast Cancer Cells T47D

https://doi.org/10.22146/ijc.91077

Undri Rastuti^(1*), Hartiwi Diastuti⁽²⁾, Senny Widyaningsih⁽³⁾, Moch Chasani⁽⁴⁾, Cindi Sheiliyani⁽⁵⁾, Anisa Rahmasari⁽⁶⁾, Bunga Sita Roihanul Fajriyah⁽⁷⁾, Puspa Rahma Mesayu⁽⁸⁾, Ranti Kamila Habibie⁽⁹⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(6) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(7) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(8) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 61, Karangwangkal, Purwokerto 53123, Indonesia
(9) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract

Nutmeg (Myristica fragrans Houtt) is a widely known spice plant, which has been reported to offer several benefits. Therefore, this study aims to develop and analyze nanoemulsions of nutmeg leaves and fruit essential oil, as well as determine their toxicity and cytotoxicity. Nanoemulsions were formulated with varying concentrations of essential oil, including 0, 1, 2, 4, and 6%. Characterization included organoleptic assessment, pH measurement, type examination, viscosity testing, transmittance analysis, particle size distribution measurement, centrifugation, and freeze-thaw cycle test. Toxicity testing results using the brine shrimp lethality test (BSLT) showed that nanoemulsions were toxic except NF F4 with high toxicity. Cytotoxicity testing on T47D breast cancer cells showed moderate activity for NF F4 nanoemulsions (IC₅₀: 34.363 ppm), while NL nanoemulsions were deemed inactive (IC₅₀: 33576.430 ppm). In addition, the organoleptic characteristics of all nanoemulsions were stable, and most parameters met the desired standards. Based on the results, further studies exploring nanoemulsions with natural products must be carried out to determine their advantages, specifically in the development of sciences.

Keywords

nanoemulsion; essential oil of nutmeg fruits and leaves; toxicity; breast cancer cells T47D; Artemia salina Leach

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References

[1] Ferlay, J., Ervik, M., Lam, F., Laversanne, M., Colombet, M., Mery, L., Piñeros, M., Znaor, A., Soerjomataram, I., Bray, F., 2020, Global Cancer Observatory: Cancer Today, International Agency for Research on Cancer, WHO, Lyon, France, https://gco.iarc.who.int/media/globocan/factsheets/populations/360-indonesia-fact-sheet.pdf, accessed on 29 August 2023.

[2] Siegel, R.L., Miller, K.D., Wagle, N.S., and Jemal, A., 2023, Cancer statistics, CA-Cancer J. Clin., 73 (1), 17–48.

[3] Tewari, D., Rawat, P., and Singh, P.K., 2018, Adverse drug reactions of anticancer drugs derived from natural sources, Food Chem. Toxicol., 123, 522–535.

[4] Seneme, E.F., dos Santos, D.C., Silva, E.M.R., Franco., E.M., and Longato, G.B., 2021, Pharmacological and therapeutic potential of myristicin: A literature review, Molecules, 26 (19), 5914.

[5] Purba, H.B., Yusufi, E.S., and Hestina, J., 2021, Performance and competitiveness of nutmeg in export market, E3S Web Conf., 232, 02018.

[6] Rastuti, U., Chasani, M., Widyaningsih, S., Habibie, R.K., Elly, V.F.R., and Zia, S.K., 2023, Formulation, characterization, and sunscreen potential evaluation of nutmeg leaf essential oil nanoemulsions (Myristica fragrans Houtt.), Molekul, 18 (3), 351–359.

[7] Cimino, C., Maurel, O.M., Musumeci, T., Bonaccorso, A., Drago, F., Souto, E.M., Pignatello, R., and Carbone, C., 2021, Essential oils: Pharmaceutical applications and encapsulation strategies into lipid-based delivery systems, Pharmaceutics, 13 (3), 327.

[8] Chikte, A.A., Umekar, M.J., Minal, N., Borkhade, and Raut, N.S., 2018, Effect of trans-esterification of oil on nanoemulsion gel for topical drug delivery, Int. J. Pharm. Sci. Res., 9 (2), 589–598.

[9] Shaker, D.S., Ishak, R.A.H., Ghoneim, A., and Elhuoni, M.A., 2019, Nanoemulsion: A review on mechanisms for the transdermal delivery of hydrophobic and hydrophilic drugs, Sci. Pharm., 87 (3), 17.

[10] Jaiswal, M., Dudhe, R., and Sharma, P.K., 2015, Nanoemulsion: An advanced mode of drug delivery system, 3 Biotech, 5 (2), 123–127.

[11] Sheiliyani, C., Hidayat, A.N., Rastuti, U., Chasani, M., and Diastuti, H, 2023, Formulation and nanoemulsion of nutmeg essential oil (Myristica fragrans Houtt.) and sunscreen activity test using UV-vis spectrophotometry, SPIN-Jurnal Kimia & Pendidikan Kimia, 5 (2), 220–230.

[12] Bakri, D., Muhtadi, M., and Wikantyasning, E., 2023, Formulation and characterization of nanoemulsion from traditional extracts for antidiabetic, Indonesia. J. Global Health Res., 5 (3), 441–448.

[13] Gurpreet, K., and Singh, S.K., 2018, Review of nanoemulsion formulation and characterization techniques, Indian J. Pharm. Sci., 80 (5), 781–789.

[14] Ernoviya, E., and Nurpermatasari, A., 2020, Formulasi dan evaluasi nanoemulsion ketokonazole, Jurnal Dunia Farmasi, 4 (3), 138–148.

[15] Saberi, A.H., Fang, Y., and McClements, D.J., 2013, Fabrication of vitamin E-enriched nanoemulsions: Factors Affecting Particle Size Using Spontaneous Emulsification, J. Colloid Interface Sci., 391, 95–102.

[16] Kumar, M., Bishnoi, R.S., Shukla, A.K., and Jain, C.P., 2019, Techniques for the formulation of nanoemulsion drug delivery system: A review, Prev. Nutr. Food Sci., 24 (3), 225–234.

[17] Aini, N.N., Wijayatri, R., and Pribadi, P, 2022, Nanoemulsion characteristics of ethanol extract from leaves of various plants: A literature review, J. Farm. Sains Praktis, 8 (3), 215–226.

[18] Stetefeld, J., McKenna, S.A., and Patel, T.R., 2016, Dynamic light scattering: A practical guide and applications in biomedical sciences, Biophys. Rev., 8 (4), 409-427.

[19] Centeri, C., Jakab, G., Szabó, S., Farsang A., Barta, K., Szalai, Z., and Bíró, Z, Comparison of particle-size analyzing laboratory methods, Environ. Eng. Manage. J., 14 (5), 1125-1135.

[20] Kerlin, M., Balboni, E., and Kim, K., 2022, Characterization, Chemistry, and Particle Size Distribution of Fallout Particles Isolated from Filter Samples, Technical Report, Lawrence Livermore National Laboratory, Livermore, CA, US.

[21] Andini, A., Prayekti, E., Wulandari, D.D., and Nidianti, E., 2020, Cytotoxicity assay using brine shrimp lethality test on collagen-chitosan wound dressing sterilized by ultraviolet light, Indones. J. Med. Lab. Sci. Technol., 2 (1), 21–26.

[22] Ashokkumar, K., Simal-Gandara, J., Murugan, M., Dhaya, K.M., and Pandian, A., 2022, Nutmeg (Myristica fragrans Houtt.) essential oil: A review on its composition, biological, and pharmacological activities, Phytother. Res., 36 (7), 2839–2851.

[23] Behzad, S., Pirani, A., and Mosaddegh, M., 2014, Cytotoxic activity of some medicinal plants from Hamedan district of Iran, Iran. J. Pharm. Res., 13, 199–205.

[24] Widiandani, T., Tandian, T., Zufar, B.D., Suryadi, A., Purwanto, B.T., Hardjono, S., and Siswandono, S., 2023, In vitro study of pinostrobin propionate and pinostrobin butyrate: cytotoxic activity against breast cancer cell T47D and its selectivity index, J Public Health Afr., 14 (Suppl. 1), 2516.

DOI: https://doi.org/10.22146/ijc.91077