Antibiofilm Efficiency of CaF2/TiO2 Strontium Borate Bioactive Glass Composites against Pseudomonas aeruginosa and Gamma Radiation Effect

https://doi.org/10.22146/ijc.84412

Eman Mohamed Abou Hussein(1), Noha Mohamed Abou Hussien(2), Sabrin Ragab Mohamed Ibrahim(3*), Mahmoud Abdelkhalek Elfaky(4), Tamer Dawod Abdelaziz(5)

(1) Radiation Chemistry Department, National Center for Radiation Research and Technology, Atomic Energy Authority, P.O. Box 8029, Nasr City, Cairo 11371, Egypt
(2) Department of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia 13829, Egypt
(3) Department of Chemistry, Preparatory Year Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
(4) Center for Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
(5) Department of Chemistry, Preparatory Year Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia
(*) Corresponding Author

Abstract


Microbial drug resistance has emerged as one of the most fundamental health threats. The current work aims to assess the antibacterial and antibiofilm potential of strontium borate bio-glasses (BBGs). Three CaF2/TiO2 strontium borate compositions have been prepared through melting annealing methods. The XRD pattern displays the amorphous nature of the glassy samples. The primary structural components of the borate, the trigonal BO3 and tetrahedral BO4 group, can be observed in FTIR spectra. Sharpness and shifting peaks to longer wavenumbers were evident after 40 kGy of gamma radiation. In contrast, density and molar volume (Vm) reveal an obvious change after irradiation. The agar diffusion technique was conducted as a preliminary screening of the antibacterial activity against Pseudomonas aeruginosa. The studied samples possessed no antimicrobial activity toward this strain; however, samples with 2% CaF2 strontium borate (T1) and 5% TiO2 strontium borate (T3) had higher biofilm inhibition potential (inhibition percentages of 75.17 and 65.77%, respectively). The gamma irradiation procedure had an unexpected detrimental effect on the bio-glass antibiofilm activity, making it unsuitable for use in sterilization procedures. Collectively, BBGs could be further investigated as possible antibacterial agents against biofilm-producing resistant strains.


Keywords


borate bioglasses; FTIR; antibacterial; Pseudomonas aeruginosa; biofilm inhibition



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DOI: https://doi.org/10.22146/ijc.84412

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