Antibiofilm Efficiency of CaF2/TiO2 Strontium Borate Bioactive Glass Composites against Pseudomonas aeruginosa and Gamma Radiation Effect

https://doi.org/10.22146/ijc.84412

Eman Mohamed Abou Hussein(1), Noha Mohamed Abou Hussien(2), Sabrin Ragab Mohamed Ibrahim(3*), Mahmoud Abdelkhalek Elfaky(4), Tamer Dawod Abdelaziz(5)

(1) Radiation Chemistry Department, National Center for Radiation Research and Technology, Atomic Energy Authority, P.O. Box 8029, Nasr City, Cairo 11371, Egypt
(2) Department of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia 13829, Egypt
(3) Department of Chemistry, Preparatory Year Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
(4) Center for Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
(5) Department of Chemistry, Preparatory Year Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia
(*) Corresponding Author

Abstract


Microbial drug resistance has emerged as one of the most fundamental health threats. The current work aims to assess the antibacterial and antibiofilm potential of strontium borate bio-glasses (BBGs). Three CaF2/TiO2 strontium borate compositions have been prepared through melting annealing methods. The XRD pattern displays the amorphous nature of the glassy samples. The primary structural components of the borate, the trigonal BO3 and tetrahedral BO4 group, can be observed in FTIR spectra. Sharpness and shifting peaks to longer wavenumbers were evident after 40 kGy of gamma radiation. In contrast, density and molar volume (Vm) reveal an obvious change after irradiation. The agar diffusion technique was conducted as a preliminary screening of the antibacterial activity against Pseudomonas aeruginosa. The studied samples possessed no antimicrobial activity toward this strain; however, samples with 2% CaF2 strontium borate (T1) and 5% TiO2 strontium borate (T3) had higher biofilm inhibition potential (inhibition percentages of 75.17 and 65.77%, respectively). The gamma irradiation procedure had an unexpected detrimental effect on the bio-glass antibiofilm activity, making it unsuitable for use in sterilization procedures. Collectively, BBGs could be further investigated as possible antibacterial agents against biofilm-producing resistant strains.


Keywords


borate bioglasses; FTIR; antibacterial; Pseudomonas aeruginosa; biofilm inhibition



References

[1] Salehi, B., Abu-Darwish, M.S., Tarawneh, A.H., Cabral, C., Gadetskaya, A.V., Salgueiro, L., Hosseinabadi, T., Rajabi, S., Chanda, W., Sharifi-Rad, M., Mulaudzi, R.B., Ayatollahi, S.A., Kobarfard, F., Arserim-Uçar, D.K., Sharifi-Rad, J., Ata, A., Baghalpour, N., and Contreras, M.M., 2019, Thymus spp. plants - Food applications and phytopharmacy properties, Trends Food Sci. Technol., 85, 287–306.

[2] Impey, R.E., Hawkins, D.A., Sutton, J.M., and Soares da Costa, T.P., 2020, Overcoming intrinsic and acquired resistance mechanisms associated with the cell wall of Gram-negative bacteria, Antibiotics, 9 (9), 623.

[3] Ibargüen-Mondragón, E., Romero-Leiton, J.P., Esteva, L., Cerón Gómez, M., and Hidalgo-Bonilla, S.P., 2019, Stability and periodic solutions for a model of bacterial resistance to antibiotics caused by mutations and plasmids, Appl. Math. Modell., 76, 238–251.

[4] Matharu, R.K., Charani, Z., Ciric, L., Illangakoon, U.E., and Edirisinghe, M., 2018, Antimicrobial activity of tellurium-loaded polymeric fiber meshes, J. Appl. Polym. Sci., 135 (25), 46368.

[5] Ottomeyer, M., Mohammadkah, A., Day, D., and Westenberg, D., 2016, Broad-spectrum antibacterial characteristics of four novel borate-based bioactive glasses, Adv. Microbiol., 6 (10), 776–787.

[6] Zhou, P., Garcia, B.L., and Kotsakis, G.A., 2022, Comparison of antibacterial and antibiofilm activity of bioactive glass compounds S53P4 and 45S5, BMC Microbiol., 22 (1), 212.

[7] Fernandes, J.S., Gentile, P., Pires, R.A., Reis, R.L., and Hatton, P.V., 2017, Multifunctional bioactive glass and glass-ceramic biomaterials with antibacterial properties for repair and regeneration of bone tissue, Acta Biomater., 59, 2–11.

[8] Drago, L., Toscano, M., and Bottagisio, M., 2018, Recent evidence on bioactive glass antimicrobial and antibiofilm activity: A minireview, Materials, 11 (2), 326.

[9] El-Tablawy, S., Abd-Allah, W., and Araby, E., 2018, Efficacy of irradiated bioactive glass 45S5 on attenuation of microbial growth and eradication of biofilm from AISI 316 L discs: In-vitro study, Silicon, 10 (3), 931–942.

[10] El-Batal, H., El-Kheshen, A.A., El-Bassyouni, G.T., and Abd El Aty, A.A., 2018, In vitro bioactivity behavior of some borate glasses and their glass-ceramic derivatives containing Zn2+, Ag+ or Cu2+ by immersion in phosphate solution and their anti-microbial activity, Silicon, 10 (3), 943–957.

[11] Moghanian, A., Ghorbanoghli, A., Kazem-Rostami, M., Pazhouheshgar, A., Salari, E., Saghafi Yazdi, M., Alimardani, T., Jahani, H., Sharifian Jazi, F., and Tahriri, M., 2020, Novel antibacterial Cu/Mg-substituted 58S-bioglass: Synthesis, characterization, and investigation of in vitro bioactivity, Int. J. Appl. Glass Sci., 11 (4), 685–698.

[12] Valappil, S.P., and Higham, S.M., 2014, Antibacterial effect of gallium and silver on Pseudomonas aeruginosa treated with gallium–silver–phosphate-based glasses, Bio-Med. Mater. Eng., 24 (3), 1589–1594.

[13] Esfahanizadeh, N., Nourani, M.R., Bahador, A., Akhondi, N., and Montazeri, M., 2018, The anti-biofilm activity of nanometric zinc doped bioactive glass against putative periodontal pathogens: An in vitro study, Biomed. Glasses, 4 (1), 95–107.

[14] Abou Neel, E.A., Hossain, K.M.Z., Abuelenain, D., Abuhaimed, T., Ahmed, I., Valappil, S.P., and Knowles, J.C., 2021, Antibacterial effect of titanium dioxide-doped phosphate glass microspheres filled total-etch dental adhesive on S. mutans biofilm, Int. J. Adhes. Adhes., 108, 102886.

[15] Bari, A., Bloise, N., Fiorilli, S., Novajra, G., Vallet-Regí, M., Bruni, G., Torres-Pardo, A., González-Calbet, J.M., Visai, L., and Vitale-Brovarone, C., 2017, Copper-containing mesoporous bioactive glass nanoparticles as multifunctional agent for bone regeneration, Acta Biomater., 55, 493–504.

[16] Zhou, J., Wang, H., Zhao, S., Zhou, N., Li, L., Huang, W., Wang D., and Zhang, C., 2016, In vivo and in vitro studies of borate-based glass micro-fibers for dermal repairing, Mater. Sci. Eng., C, 60, 437–445.‏

[17] Maany, D.A., Alrashidy, Z.M., Abdel Ghany, N.A., and Abdel-Fattah, W.I., 2019, Comparative antibacterial study between bioactive glasses and vancomycin hydrochloride against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, Egypt Pharm. J., 18 (4), 304–310.

[18] Han, T., Stone-Weiss, N., Huang, J., Goel, A., and Kumar, A., 2020, Machine learning as a tool to design glasses with controlled dissolution for healthcare applications, Acta Biomater., 107, 286–298.‏

[19] Abd-Allah, W.M., and Fathy, R.M., 2022, Gamma irradiation effectuality on the antibacterial and bioactivity behavior of multicomponent borate glasses against methicillin-resistant Staphylococcus aureus (MRSA), JBIC, J. Biol. Inorg. Chem., 27 (1), 155–173.

[20] Hall, C.W., and Mah, T.F., 2017, Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria, FEMS Microbiol. Rev., 41 (3), 276–301.

[21] Ciofu, O., and Tolker-Nielsen, T., 2019, Tolerance and resistance of Pseudomonas aeruginosa biofilms to antimicrobial agents—How P. aeruginosa can escape antibiotics, Front. Microbiol., 10, 913.

[22] İdil, Ö., Şahal, H., Canpolat, E., and Özkan, M., 2023, Synthesis, characterization, antimicrobial and time killing activities of new sulfa-derived Schiff bases coordinated with Cu(II), Indones. J. Chem., 23 (3), 831–842.

[23] da Silva Aquino, K.A., 2012, “Sterilization by gamma irradiation” in Gamma Radiation, Eds. Adrovic, F., IntechOpen, Rijeka, Croatia, 172–202.

[24] Chanshetti, U.B., Shelke, V.A., Jadhav, S.M., Shankarwar, S.G., Chondhekar, T.K., Shankarwar, A.G., Sudarsan, V., and Jogad, M.S., 2011, Density and molar volume studies of phosphate glasses, FU Phys. Chem. Technol., 9 (1), 29–36.

[25] Clinical Laboratory Standard Institute, 2006, Zone diameter Interpretive Standards and corresponding minimal inhibitory concentration (MIC) interpretive break point, Supplement M44-S1, Clinical and Laboratory Standard Institute, Wayne, PA.

[26] Madhu, A., Eraiah, B., and Srinatha, N., 2020, Gamma irradiation effects on the structural, thermal, and optical properties of samarium doped lanthanum–lead-boro-tellurite glasses, J. Lumin., 221, 117080.

[27] Fernandes, J.S., Gentile, P., Moorehead, R., Crawford, A., Miller, C.A., Pires, R.A., Hatton, P.V., and Reis, R.L., 2016, Design and properties of novel substituted borosilicate bioactive glasses and their glass-ceramic derivatives, Cryst. Growth Des., 16 (7), 3731–3740.‏

[28] Swansbury, L.A., 2017, A Structural Investigation of Chlorine-Containing and Fluorine-Containing Oxide Glasses Using Molecular Dynamics, Neutron Diffraction, and X-ray Absorption Spectroscopy Dissertation, University of Kent, UK.‏

[29] Almuqrin, A.H., Kumar, A., Prabhu, N.S., Jecong, J.F.M., Kamath, S.D., and Abu Al-Sayyed, M.I., 2022, Mechanical and gamma-ray shielding examinations of Bi2O3–PbO–CdO–B2O3 glass system, Open Chem., 20 (1), 808–815.

[30] Abou Hussein, E.M., Madbouly, A.M., Ezz Eldin, F.M., and ElAlaily. N.A., 2021, Evaluation of physical and radiation shielding properties of Bi2O3–B2O3 glass doped transition metals ions, Mater. Chem. Phys., 261, 124212.

[31] Abou Hussein, E.M., 2019, Characterization of some chemical and physical properties of lithium borate glasses doped with CuO and/or TeO2, J. Chem. Soc. Pak., 41, 52–61.

[32] Mohan, S., Kaur, S., Singh, D.P., and Kaur, P., 2017, Structural and luminescence properties of samarium doped lead alumino borate glasses, Opt. Mater., 73, 223–233.

[33] Abou Hussein, E.M., El-Agawany, F.I., and Rammah, Y.S., 2022, CuO reinforced lithium-borate glasses: Fabrication, structure, physical properties, and ionizing radiation shielding competence, J. Aust. Ceram. Soc., 58 (1), 157–169.

[34] Abou Hussein, E.M., 2019, Vitrified municipal waste for the immobilization of radioactive waste: preparation and characterization of borosilicate glasses modified with metal oxides, Silicon, 11 (6), 2675–2688.

[35] Abou Hussein, E.M., and Barakat, M.A.Y., 2022, Structural, physical and ultrasonic studies on bismuth borate glasses modified with Fe2O3 as promising radiation shielding materials, Mater. Chem. Phys., 290, 126606.

[36] El Batal, H.A., Abou Hussein, E.M., El Alaily, N.A., and EzzEldin, F.M., 2020, Effect of different 3d transition metal oxides on some physical properties of γ-Irradiated Bi2O3- B2O3 glasses: A comparative study, J. Non-Cryst. Solids, 528, 119733.

[37] Ahmad, Z., Ali, S., Ahmad, H., Hayat, K., Iqbal, Y., Zulfiqar, S., Zaman, F., Rooh, G., and Kaewkhao, J., 2020, Radio-optical response of cerium-doped lithium gadolinium bismuth borate glasses, J. Lumin., 224, 117341.

[38] Abd El-Rehim, A.F., Shaaban, K.S., Zahran, H.Y., Yahia, I.S., Ali, A.M., Abou Halaka, M.M., Makhlouf, S.A., Abdel Wahab, E.A., and Shaaban, E.R., 2021, Structural and mechanical properties of lithium bismuth borate glasses containing molybdenum (LBBM) together with their glass–ceramics, J. Inorg. Organomet. Polym. Mater., 31 (3), 1057–1065.‏

[39] Halimah, M.K., Chiew, W.H., Sidek, H.A.A., Daud, W.M., Wahab, Z.A., Khamirul, A.M., and Iskandar, S.M., 2014, Optical properties of lithium borate glass (Li2O)x(B2O3), Sains Malays., 43 (6), 899–902.‏

[40] Abou Hussein, E.M., 2023, The impact of electron beam irradiation on some novel borate glasses doped V2O5; Optical, physical and spectral investigation, Inorg. Chem. Commun., 147, 110232.

[41] El‑Alaily, N.A., Abou Hussein, E.M., and Ezz Eldin, F.M., 2018, Gamma irradiation and heat treatment effects on barium borosilicate glasses doped titanium oxide, J. Inorg. Organomet. Polym. Mater., 28, 2662–2676.

[42] Bhogi, A., Vijaya Kumar, R., and Kistaiah, P., 2015, Effect of alkaline earths on spectroscopic and structural properties of Cu2+ ions-doped lithium borate glasses, J. Non-Cryst. Solids, 426, 47–54.

[43] Paramesh, G., and Varma, K.B.R., 2013, Structure‐property correlation in BaO‐TiO2‐B2O3 glasses: Glass stability, optical, hydrophobic, and dielectric properties, Int. J. Appl. Glass Sci., 4 (3), 248–255.

[44] Christensen, G.D., Simpson, W.A., Bisno, A.L., and Beachey, E.H., 1982, Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces, Infect. Immun., 37 (1), 318–326.

[45] Farag, M.M., Abd-Allah, W.M., and Ahmed, H.Y.A., 2017, Study of the dual effect of gamma irradiation and strontium substitution on bioactivity, cytotoxicity, and antimicrobial properties of 45S5 bioglass, J. Biomed. Mater. Res., Part A, 105 (6), 1646–1655.

[46] Wilkinson, H.N., Iveson, S., Catherall, P., and Hardman. M.J., 2018, A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model, Front. Microbiol., 9, 1450.

[47] Fayad, A.M., Fathi, A.M., El-Beih, A.A., Taha, M.A., and Abdel-Hameed, S.A.M., 2019, Correlation between antimicrobial activity and bioactivity of Na-mica and Na-mica/fluorapatite glass and glass-ceramics and their corrosion protection of titanium in simulated body fluid, J. Mater. Eng. Perform., 28 (9), 5661–5673.‏

[48] Abdelghany, A.M., and Kamal, H., 2014, Spectroscopic investigation of synergetic bioactivity behavior of some ternary borate glasses containing fluoride anions, Ceram. Int., 40 (6), 8003–8011.‏

[49] Elbatal, H.A., Azooz, M.A., Saad, E.A., EzzELDin, F.M., and Amin, M.S., 2018, Corrosion behavior mechanism of borosilicate glasses towards different leaching solutions evaluated by the grain method and FTIR spectral analysis before and after gamma irradiation, Silicon, 10 (3), 1139–1149.



DOI: https://doi.org/10.22146/ijc.84412

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