Development of a Rapid and Sensitive Probe for Colorimetric Detection of Ni2+ Ion in Water Sample by β-Cyclodextrin Stabilized Silver Nanoparticles

Farrah Nurkhaliza(1), Ahmad Fathoni(2), Muhammad Eka Prastya(3), Zetryana Puteri Tachrim(4), Abdul Aji(5), Agustina Sus Andreani(6*)

(1) Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Building 452, Serpong, Banten 15314, Indonesia; Department of Chemistry, Syarif Hidayatullah State Islamic University, Jl. Ir. H. Djuanda No. 95, Ciputat, Banten 15412, Indonesia
(2) Department of Chemistry, Syarif Hidayatullah State Islamic University, Jl. Ir. H. Djuanda No. 95, Ciputat, Banten 15412, Indonesia
(3) Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Building 466, Serpong, Banten 15314, Indonesia
(4) Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Serpong, Banten 15314, Indonesia
(5) Department of Chemistry, Institut Teknologi Sumatera, Jl. Terusan Ryacudu, Jati Agung, Lampung 35365, Indonesia
(6) Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Kawasan Puspiptek, Building 452, Serpong, Banten 15314, Indonesia
(*) Corresponding Author


A rapid and selective colorimetric detection of Ni2+ was developed using silver nanoparticles (AgNPs) with β-cyclodextrin (β-CDs) as reducing and stabilizing agents. Characterization was assessed by spectrophotometer UV-vis, Fourier transform infra-red (FTIR), transmission electron microscopy (TEM), and particle size analyzer (PSA). The AgNPs-β-CDs were relatively stable after being stored for 5 months. The addition of Ni2+ to the AgNPs-β-CDs shifted the surface plasmon resonance (SPR) band at 409 nm. Synthesized AgNPs-β-CDs had a spherical shape and an average size of 25.07 ± 0.66 nm (analyzed by TEM) and 33.63 ± 0.25 nm, as confirmed by PSA. AgNPs-β-CDs as colorimetric sensors for Ni2+ ions had a good linear calibration curve at 409 nm with the R2 value of 0.9993. The limit of detection (LoD) was found to be 33.30 ppb, while the limit of quantification (LoQ) was 111.0 ppb. This sensor had been applied to a seawater sample from Ancol Beach, North Jakarta, Indonesia and it exhibited good precision and accuracy. In this work, β-CDs-synthesized AgNPs were able to detect Ni2+ ions and were beneficial as an alternative method for Ni2+ screening in environmental samples.


AgNPs; β-cyclodextrin; colorimetric sensor; Ni2+

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[1] Tokay, F., and Bağdat, S., 2018, Preconcentration and determination of metal ions using fluorescein-modified silica gel and inductively coupled plasma-optical emission spectrometry, Anal. Lett., 51 (1-2), 119–132.

[2] Akcin, N., Koyuncu, I., and Akcin, G., 2011, Determination of zinc, nickel and cadmium in natural water samples by flame atomic absorption spectrometry after preconcentration with ion exchange and flotation techniques, Rev. Anal. Chem., 30 (2), 65–71.

[3] Tu, L.N., Van Tan, L., and Chien, N.X., 2013, Simultaneous spectrophotometric determination of Ni(II) and Zn(II) in waste water by H-‎point addition standard method using 5-bromosalicylaldehyde thiosemicarbazone, Eur. Chem. Bull., 2 (6), 311–315.

[4] Rossi, A., Zannotti, M., Cuccioloni, M., Minicucci, M., Petetta, L., Angeletti, M., and Giovannetti, R., 2021, Silver nanoparticle-based sensor for the selective detection of nickel ions, Nanomaterials, 11 (7), 1733.

[5] Azimi, H., Ahmadi, S.H., Manafi, M.R., Husain, S., Mousavi, H., and Najafi, M., 2021, Development a simple and sensitive method for determination low trace of nickel by localized surface plasmon resonance of citrate capped silver nanoparticles, J. Optoelectron. Nanostruct., 6 (2), 23–40.

[6] Milczarek, G., Rebis, T., and Fabianska, J., 2013, One-step synthesis of lignosulfonate-stabilized silver nanoparticles, Colloids Surf., B, 105, 335–341.

[7] Bindhu, M.R., and Umadevi, M., 2014, Spectroscopy Silver and gold nanoparticles for sensor and antibacterial applications, Spectrochim. Acta, Part A, 128, 37–45.

[8] Chouhan, S., and Guleria, S., 2020, Green synthesis of AgNPs using Cannabis sativa leaf extract: Characterization, antibacterial, anti-yeast and α-amylase inhibitory activity, Mater. Sci. Energy Technol., 3, 536–544.

[9] Daoudi, K., Ramachandran, K., Alawadhi, H., Boukherroub, R., Dogheche, E., Khakani, M.A.E., and Gaidi, M., 2021, Ultra-sensitive and fast optical detection of the spike protein of the SARS-CoV-2 using AgNPs/SiNWs nanohybrid based sensors, Surf. Interfaces, 27, 101454.

[10] Bogireddy, N.K.R., Kiran Kumar, H.A., and Mandal, B.K., 2016, Biofabricated silver nanoparticles as green catalyst in the degradation of different textile dyes, J. Environ. Chem. Eng., 4 (1), 56–64.

[11] Nguyen, T.D., Dang, C.H., and Mai, D.T., 2018, Biosynthesized AgNP capped on novel nanocomposite 2-hydroxypropyl-β-cyclodextrin/alginate as a catalyst for degradation of pollutants, Carbohydr. Polym. 197, 29–37.

[12] Mochi, F., Burratti, L., Fratoddi, I., Venditti, I., Battocchio, C., Carlini, L., Iucci, G., Casalboni, M., De Matteis, F., Casciardi, S., Nappini, S., Pis, I., and Prosposito, P., 2018, Plasmonic sensor based on interaction between silver nanoparticles and Ni2+ or Co2+ in water, Nanomaterials, 8 (7), 488.

[13] Oluwafemi, O.S., Anyik, J.L., Zikalala, N.E., and Sakho, E.H.M., 2019, Biosynthesis of silver nanoparticles from water hyacinth plant leaves extract for colourimetric sensing of heavy metals, Nano-Struct. Nano-Objects, 20, 100387.

[14] Pethakamsetty, L., Kothapenta, K., Nammi, H.R., Ruddaraju, L.K., Kollu, P., Yoon, S.G., and Pammi, S.V.N., 2016, Green synthesis, characterization and antimicrobial activity of silver nanoparticles using methanolic root extracts of Diospyros sylvatica, J. Environ. Sci., 55, 157–163.

[15] Andreani, A.S., Kunarti, E.S., Hashimoto, T., Hayashita, T., and Santosa, S.J., 2021, Fast and selective colorimetric detection of Fe3+ based on gold nanoparticles capped with ortho-hydroxybenzoic acid, J. Environ. Chem. Eng., 9 (5), 105962.

[16] Veglia, A.V., and Bracamonte, A.G., 2019, β-Cyclodextrin grafted gold nanoparticles with short molecular spacers applied for nanosensors based on plasmonic effects, Microchem. J., 148, 277–284.

[17] Ilanchelian, M., Retna Raj, C., and Ramaraj, R., 2000, Spectral studies on the Cyclodextrin inclusion complexes of toluidine blue O and Meldola’s blue in aqueous solution, J. Inclusion Phenom. Macrocyclic Chem., 36 (1), 9–20.

[18] Premkumar, T., and Geckeler, K.E., 2014, Facile synthesis of silver nanoparticles using unmodified cyclodextrin and their surface-enhanced Raman scattering activity, New J. Chem., 38 (7), 2847–2855.

[19] Rajamanikandan, R., and Ilanchelian, M., 2018, β-Cyclodextrin functionalised silver nanoparticles as a duel colorimetric probe for ultrasensitive detection of Hg2+ and S2− ions in environmental water samples, Mater. Today Commun., 15, 61–69.

[20] Ma, Q., Song, J., Zhang, S., Wang, M., Guo, Y., and Dong, C., 2016, Colorimetric detection of riboflavin by silver nanoparticles capped with β-cyclodextrin-grafted citrate, Colloids Surf., B, 148, 66–72.

[21] Qiu, X., Gu, J., Yang, T., Ma, C., Li, L., Wu, Y., Zhu, C., Gao, H., Yang, Z., Wang, Z., Li, X., Hu, A., Xu, J., Zhong, L., Shen, J., Huang, A., and Chen, G., 2022, Sensitive determination of Norfloxacin in milk based on β-cyclodextrin functionalized silver nanoparticles SERS substrate, Spectrochim. Acta, Part A, 276, 121212.

[22] Suárez-Cerda, J., Nuñez, G. A., Espinoza-Gómez, H., and Flores-López, L. Z., 2014, A comparative study of the effect of α-, β-, and γ-cyclodextrins as stabilizing agents in the synthesis of silver nanoparticles using a green chemistry method, Mater. Sci. Eng. C, 43, 21–26.

[23] Nurkhaliza, F., Fathoni, A., Yati, I., Prastya, M.E., Jenie, S.N.A., and Andreani, A.S., 2023, UV-Vis study on β-cyclodextrin as dual function for synthesis AgNPs and antibacterial application, Macromol. Symp., 409 (1), 2200182.

[24] Yu, Y., Wang, Q., Yuan, J., Fan, X., and Wang, P., 2016, A novel approach for grafting of β-cyclodextrin onto wool via laccase/TEMPO oxidation, Carbohydr. Polym., 153, 463–470.

[25] Ndikau, M., Noah, N.M., Andala, D.M., and Masika, E., 2017, Green synthesis and characterization of silver nanoparticles using Citrullus lanatus fruit rind extract, Int. J. Anal. Chem., 2017, 8108504.

[26] Dodero, A., Schlatter, G., Hébraud, A., Vicini, S., and Castellano, M., 2021, Polymer-free cyclodextrin and natural polymer-cyclodextrin electrospun nanofibers: A comprehensive review on current applications and future perspectives, Carbohydr. Polym., 264, 118042.

[27] Aji, A., Santosa, S.J., and Kunarti, E.S., 2020, Effect of reaction time and stability properties of gold nanoparticles synthesized by p-aminobenzoic acid and p-aminosalicylic acid, Indones. J. Chem., 20 (2), 413–421.

[28] He, J., Li, Y., Wang, C., Zhang, K., Lin, D., Kong, L., and Liu, J., 2017, Rapid adsorption of Pb, Cu and Cd from aqueous solutions by β-cyclodextrin polymers, Appl. Surf. Sci., 426, 29–39.

[29] Ngamchuea, K., Batchelor-McAuley, C., Sokolov, S.V., and Compton, R.G., 2017, Dynamics of silver nanoparticles in aqueous solution in the presence of metal ions, Anal. Chem., 89 (19), 10208–10215.

[30] Sulistiawaty, L., Sugiarti, S., and Darmawan, N., 2015, Detection of Hg2+ metal ions using silver nanoparticles stabilized by gelatin and tween-20, Indones. J. Chem., 15 (1), 1–8.

[31] Kabbur, S.M., Waghmare, S.D., Ghodake, U.R., and. Suryavanshi, S.S, 2018, Synthesis, morphology and electrical properties of Co2+ substituted NiCuZn ferrites for MLCI applications, AIP Conf. Proc., 1942 (1), 130002.

[32] Almaquer, F.E.P., Ricacho, J.S.Y., and Ronquillo, R.L.G., 2019, Simple and rapid colorimetric sensing of Ni(II) ions in tap water based on aggregation of citrate-stabilized silver nanoparticles, Sustain. Environ. Res., 29 (1), 23.

[33] Samanta, S., Das, S., and Biswas, P., 2014, Synthesis of 3,6-di(pyridin-2-yl)-1,2,4,5-tetrazine (pytz) capped silver nanoparticles using 3,6-di(pyridin-2-yl)-1,4-dihydro-1,2,4,5-tetrazine as reducing agent: Application in naked eye sensing of Cu2+, Ni2+ and Ag+ ions in aqueous solution and paper platform, Sens. Actuators, B, 202, 23–30.

[34] Feng, J., Jin, W., Huang, P., and Wu, F., 2017, Highly selective colorimetric detection of Ni2+ using silver nanoparticles cofunctionalized with adenosine monophosphate and sodium dodecyl sulfonate, J. Nanoparticle Res., 19 (9), 306.

[35] González, A.G., Herrador, M.Á., and Asuero, A.G., 2010, Intra-laboratory assessment of method accuracy (trueness and precision) by using validation standards, Talanta, 82 (5), 1995–1998.


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