Indonesian Purple Rice Ferulic Acid as a Candidate for Anti-aging through the Inhibition of Collagenase and Tyrosinase Activities

Ernanin Dyah Wijayanti(1), Anna Safitri(2), Dian Siswanto(3), Fatchiyah Fatchiyah(4*)

(1) Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia; Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia; Health Polytechnique of Putra Indonesia Malang, Jl. Barito 5, Malang 65123, East Java, Indonesia
(2) Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
(3) Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
(4) Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia; Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
(*) Corresponding Author


Skin aging is associated with decreased skin firmness and excessive pigmentation, which is caused by the activity of aging enzymes. This process can be prevented with powerful antioxidants from nature, such as ferulic acid which is abundant in rice. This study examines the nutritional content and phytochemicals of Indonesian purple rice and evaluates the bioactivity of ferulic acid as an anti-aging agent. Indonesian purple rice has less fat than black and white rice, more amino acids involved in aging regulation, and a similar phytochemical profile to black and white rice. Indonesian purple rice has a lower concentration of ferulic acid (4.114 ± 0.013 mg/L) than black rice but shows strong reducing power (IC50 9.35 ± 1.95 µg/mL), high anti-tyrosinase (IC50 59.57 ± 3.60 µg/mL), and moderate anti-collagenase activities (IC50 74.18 ± 3.11 µg/mL). This study supports the use of Indonesian purple rice as a promising active ingredient in natural anti-aging cosmetics.


anti-aging; collagenase; ferulic acid; purple rice; tyrosinase

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[1] Girsang, E., Ginting, C.N., Lister, I.N.E., Widowati, W., Wibowo, S.H.B., Perdana, F.S., and Rizal, R., 2019, In silico analysis of phytochemical compound found in snake fruit (Salacca zalacca) peel as anti-aging agent, Thai J. Pharm. Sci., 43 (2), 105–109.

[2] Kim, M., and Park, H.J., 2016, "Molecular Mechanisms of Skin Aging and Rejuvenation" in Molecular Mechanisms of the Aging Process and Rejuvenation, Eds. Shiomi, N., IntechOpen, Rijeka, 57–76.

[3] Campa, M., and Baron, E., 2018, Anti-aging effects of select botanicals: Scientific evidence and current trends, Cosmetics, 5 (3), 54.

[4] Mukherjee, P.K., Maity, N., Nema, N.K., and Sarkar, B.K., 2011, Bioactive compounds from natural resources against skin aging, Phytomedicine, 19 (1), 64–73.

[5] Shin, S.Y., Ko, J.Y., Kim, M.J., Song, N., and Park, K.M., 2021, Morin induces melanogenesis via activation of MAPK signaling pathways in B16F10 mouse melanoma cells, Molecules, 26 (8), 2150.

[6] Apraj, V.D., and Pandita, N.S., 2016, Evaluation of skin anti-aging potential of Citrus reticulata Blanco peel, Pharmacogn. Res., 8 (3), 160–168.

[7] El-Nashar, H.A.S., El-labbad, E.M., Al-Azzawi, M.A., and Ashmawy, N.S., 2022, A new xanthone glycoside from Mangifera incida L.: Physicochemical properties and in vitro anti-skin aging activities, Molecules, 27 (9), 2609.

[8] Hashemi, S.M., and Emami, S., 2015, Kojic acid-derived tyrosinase inhibitors: Synthesis and bioactivity, Pharm. Biomed. Res., 1 (1), 1–17.

[9] Tu, P.T.B., and Tawata, S., 2015, Anti-oxidant, anti-aging, and anti-melanogenic properties of the essential oils from two varieties of Alpinia zerumbet, Molecules, 20 (9), 16723–16740.

[10] Ganceviciene, R., Liakou, A.I., Theodoridis, A., Makrantonaki, E., and Zouboulis, C.C., 2012, Skin anti-aging strategies, Derm.-Endocrinol., 4 (3), 308–319.

[11] Vijayakumar, R., Abd Gani, S.S., and Mokhtar, N.F., 2017, Anti-elastase, anti-collagenase and antimicrobial activities of the underutilized red pitaya peel: An in vitro study for anti-aging applications, Asian J. Pharm. Clin. Res., 10 (8), 251–255.

[12] Das, S., and Wong, A.B.H., 2020, Stabilization of ferulic acid in topical gel formulation via nanoencapsulation and pH optimization, Sci. Rep., 10 (1), 12288.

[13] de Paiva, L.B., Goldbeck, R., dos Santos, W.D., and Squina, F.M., 2013, Ferulic acid and derivatives: Molecules with potential application in the pharmaceutical field, Braz. J. Pharm. Sci., 49 (3), 395–411.

[14] Kumar, N., and Pruthi, V., 2014, Potential applications of ferulic acid from natural sources, Biotechnol. Rep., 4, 86–93.

[15] Alam, M.A., 2019, Anti-hypertensive effect of cereal antioxidant ferulic acid and its mechanism of action, Front. Nutr., 6, 121.

[16] Saha, S., 2016, Black rice: The new age super food (An extensive review), AIJRFANS, 16 (1), 51–55.

[17] Wijayanti, E.D., Safitri, A., Siswanto, D., and Fatchiyah, F., 2022, Genomic profile of OsCOMT in Indonesian purple rice, Biotropika, 10 (3), 185–190.

[18] Sing, S.X., Lee, H.H., Wong, S.C., Bong, C.F.J., and Yiu, P.H., 2015, Ferulic acid, gamma oryzanol and GABA content in whole grain rice and their variation with bran colour, Emir. J. Food Agric., 27 (9), 706–711.

[19] Zhang, H., Shao, Y., Bao, J., and Beta, T., 2015, Phenolic compounds and antioxidant properties of breeding lines between the white and black rice, Food Chem., 172, 630–639.

[20] Wijayanti, E.D., Safitri, A., Siswanto, D., Triprisila, L.F., and Fatchiyah, F., 2021, Antimicrobial activity of ferulic acid in Indonesian purple rice through toll-like receptor signaling, Makara J. Sci., 25 (4), 247–257.

[21] Wijayanti, E.D., Safitri, A., Siswanto, D., and Fatchiyah, F., 2022, Virtual prediction of purple rice ferulic acid as anti-inflammatory of TNF-α signaling, Berkala Penelitian Hayati, 27 (2), 59–66.

[22] Fatchiyah, F., Sari, D.R.T., Safitri, A., and Cairns, J.R.K., 2020, Phytochemical compound and nutritional value in black rice from Java Island, Indonesia, Syst. Rev. Pharm., 11 (7), 414–421.

[23] Godghate, A., Sawant, R., and Sutar, A., 2012, Phytochemical analysis of ethanolic extract of roots of Carrisa carandus Linn, Rasayan J. Chem., 5 (4), 456–459.

[24] Maimulyanti, A., Prihadi, A.R., and Safrudin, I., 2016, Chemical composition, phytochemical screening and antioxidant activity of Acmella uliginosa (Sw.) Cass leaves, Indones. J. Chem., 16 (2), 162–174.

[25] Stavova, E., Porizka, J., Stursa, V., Enev, V., and Divis, P., 2017, Extraction of ferulic acid from wheat bran through alkaline hydrolysis, MendelNet, 24, 574–579.

[26] Baba, S.A., and Malik, S.A., 2015, Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume, J. Taibah Univ. Sci., 9 (4), 449–454.

[27] Hapsari, A.M., Masfria, M., and Dalimunthe, A., 2018, Pengujian kandungan total fenol ekstrak etanol tempuyung (Shoncus arvensis L.), Talenta Conf. Ser.: Trop. Med., 1 (1), 284–290.

[28] Agustin, A.T., Safitri, A., and Fatchiyah, F., 2021, Java red rice (Oryza sativa L.) nutritional value and anthocyanin profiles and its potential role as antioxidant and anti-diabetic, Indones. J. Chem., 21 (4), 968–978.

[29] Widowati, W., Fauziah, N., Herdiman, H., Afni, M., Afifah, E., Kusuma, H.S.W., Nufus, H., Arumwardana, S., and Rihibiha, D.D., 2016, Antioxidant and anti aging assays of Oryza sativa extracts, vanillin and coumaric acid, J. Nat. Rem., 16 (3), 88–99.

[30] Kim, K.Y., Lee, E.J., Whang, W.K., and Park, C.H., 2019, In vitro and in vivo anti-aging effects of compounds isolated from Artemisia iwayomogi, J. Anal. Sci. Technol., 10 (1), 35.

[31] Girsang, E., Lister, I.N.E., Ginting, C.N., Bethasari, M., Amalia, A., and Widowati, W., 2020, Comparison of antiaging and antioxidant activities of protocatechuic and ferulic acids, MCBS, 4 (2), 68–75.

[32] Dwiwibangga, Y., Safitri, A., and Fatchiyah, F., 2022, Profiling of phytochemical compounds of East Java red rice bran has the high-value biological activities as antioxidant and antidiabetic, Indones. J. Chem., 22 (5), 1304–1320.

[33] Spencer, S.J., D’Angelo, H., Soch, A., Watkins, L.R., Maier, S.F., and Barrientos, R.M., 2017, High-fat diet and aging interact to produce neuroinflammation and impair hippocampal- and amygdalar-dependent memory, Neurobiol. Aging, 58, 88–101.

[34] Tucker, L.A., 2019, Milk fat intake and telomere length in U.S. women and men: The role of the milk fat fraction, Oxid. Med. Cell. Longevity, 2019, 1574021.

[35] Yin, Z., Raj, D.D., Schaafsma, W., van der Heijden, R.A., Kooistra, S.M., Reijne, A.C., Zhang, X., Moser, J., Brouwer, N., Heeringa, P., Yi, C.X., van Dijk, G., Laman, J.D., Boddeke, E.W.G.M., and Eggen, B.J.L., 2018, Low-fat diet with caloric restriction reduces white matter microglia activation during aging, Front. Mol. Neurosci., 11, 65.

[36] Bojarska, J., 2020, Amino acids and short peptides as anti-aging "superfood", Int. J. Nutr. Sci., 5 (1), 1039.

[37] Jana, B.R., and Idris, M., 2018, Anti-aging amino acids in Euryale ferox (Salisb.): A review, Adv. Plants Agric. Res., 8 (1), 39–43.

[38] Park, J., Jung, H., Jang, B., Song, H., Han, I., and Oh, E., 2020, D-tyrosine adds an anti-melanogenic effect to cosmetic peptides, Sci. Rep., 10 (1), 262.

[39] Sibhatu, H.K., Jabasingh, S.A., Yimam, A., and Ahmed, S., 2021, Ferulic acid production from brewery spent grains, an agro-industrial waste, LWT-Food Sci. Technol., 135, 110009.

[40] Adeyemo, O.A., Osibote, E., Adedugba, A., Bhadmus, O.A., Adeoshun, A.A., and Allison, M.O., 2018, Antioxidant activity, total phenolic contents and functional group identification of leaf extracts among lemongrass (Cymbopogon citratus) accessions, NISEB J., 18 (2), 83–91.

[41] Chen, J., Yang, J., Ma, L., Li, J., Shahzad, N., and Kim, C.K., 2020, Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids, Sci. Rep., 10 (1), 2611.

[42] Goufo, P., and Trindade, H., 2014, Rice antioxidants: Phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid, Food Sci. Nutr., 2 (2), 75–104.

[43] Doncea, S.M., Stoica, R., Ion, M.R., Trandafir, I., and Pavel, V., 2010, An HPLC method for identification and separation of some phenolic acids in the coffee, Petroleum - Gas University of Ploiesti Bulletin, Technical Series, 62 (3A), 143–148.

[44] Jabri-Karoui, I., Bettaieb, I., Msaada, K., Hammami, M., and Marzouk, B., 2012, Research on the phenolic compounds and antioxidant activities of Tunisian Thymus capitatus, J. Funct. Foods, 4 (3), 661–669.

[45] Jin, C.Y., Liu, H., Xu, D., Zeng, F.K., Zhao, Y.C., Zhang, H., and Liu, G., 2018, Glycoalkaloids and phenolic compounds in three commercial potato cultivars grown in Hebei, China, Food Sci. Hum. Wellness, 7 (2), 156–162.

[46] Weidner, S., Król, A., Karamać, M., and Amarowicz, R., 2018, Phenolic compounds and the antioxidant properties in seeds of green- and yellow-podded bean (Phaseolus vulgaris L.) varieties, CyTA-J. Food, 16 (1), 373–380.

[47] Sadeer, N.B., Montesano, D., Albrizio, S., Zengin, G., and Mahomoodally, M.F., 2020, The versatility of antioxidant assays in food science and safety-Chemistry, applications, strengths, and limitations, Antioxidants, 9 (8), 709.

[48] Kose, L.P., Bingol, Z., Kaya, R., Goren, A.C., Akincioglu, H., Durmaz, L., Koksal, E., Alwasel, S.H., and Gülçin, I., 2020, Anticholinergic and antioxidant activities of avocado (Folium perseae) leaves – phytochemical content by LC-MS/MS analysis, Int. J. Food Prop., 23 (1), 878–893.

[49] Xiao, F., Xu, T., Lu, B., and Liu, R., 2020, Guidelines for antioxidant assays for food components, Food Front., 1 (1), 60–69.

[50] Rahman, M., Islam, B., Biswas, M., and Khurshid Alam, A.H.M., 2015, In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh, BMC Res. Notes, 8 (1), 621.

[51] Syakri, S., Syahrana, N.A., Ismail, A., Tahir, K.A., and Masri, A., 2021, A review: Testing antioxidant activity on kawista plants (Limonia acidissima L.) in Indonesia, Open Access Maced. J. Med. Sci., 9 (F), 281–287.

[52] Bilska, K., Wojciechowska, N., Alipour, S., and Kalemba, E.M., 2019, Ascorbic acid: The little-known antioxidant in woody plants, Antioxidants, 8 (12), 645.

[53] de Oliveira Silva, E., and Batista, R., 2017, Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: A review on their structures, occurrence, and potential health benefits, Compr. Rev. Food Sci. Food Saf., 16 (4), 580–616.

[54] Zayova, E., Stancheva, I., Geneva, M., Petrova, M., and Dimitrova, L., 2013, Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins, Turk. J. Biol., 37 (1), 106–113.

[55] Zduńska, K., Dana, A., Kolodziejczak, A., and Rotsztejn, H., 2018, Antioxidant properties of ferulic acid and its possible application, Skin Pharmacol. Physiol., 31 (6), 332–336.

[56] Zolghadri, S., Bahrami, A., Hassan Khan, M.T., Munoz-Munoz, J., Garcia-Molina, F., Garcia-Canovas, F., and Saboury, A.A., 2019, A comprehensive review on tyrosinase inhibitors, J. Enzyme Inhib. Med. Chem., 34 (1), 279–309.

[57] Strickertsson, J.A.B., Desler, C., and Rasmussen, L.J., 2014, Impact of bacterial infections on aging and cancer: Impairment of DNA repair and mitochondrial function of host cells, Exp. Gerontol., 56, 164–174.

[58] Pokharel, S.M., Chiok, K., Shil, N.K., Mohanty, I., and Bose, S., 2021, Tumor necrosis factor-alpha utilizes MAPK/NFκB pathways to induce cholesterol-25 hydroxylase for amplifying pro-inflammatory response via 25-hydroxycholesterol-integrin-FAK pathway, PLoS One, 16 (9), e0257576.

[59] Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J., Tan, L., Gao, L., Kang, L., Cao, K., Zhou, J., Xiao, R., Chen, J., and Huang, J., 2017, MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway, Mol. Med. Rep., 15 (6), 3977–3982.

[60] Metsämuuronen, S., and Sirén, H., 2019, Bioactive phenolic compounds, metabolism, and properties: A review on valuable chemical compounds in Scots pine and Norway spruce, Phytochem. Rev., 18 (3), 623–664.


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